Chemiluminescence Molecular Detection of Sequence-Specific HBV-DNA Using Magnetic Nanoparticles

Wang, Fang; Ma, Chao; Zeng, Xin; Li, Chuanyan; Deng, Yan; He, Nongyue

doi:10.1166/jbn.2012.1446

Cited by 47 publications

(34 citation statements)

References 4 publications

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“…3, upon continuous-wave excitation at 980 nm, nanocrystals prepared by solvothermal and hydrothermal methods exhibited similar UCL spectra with an excellent 800 nm near-infrared (NIR) UCL and an weak emission band at 700 nm. The emission band peaked at 700 nm was caused by 3 [22,24]. The UCL intensities of the prepared samples were measured at the same concentration of 1.0 mg/ mL in cyclohexane.…”

Section: Upconversion Luminescence Properties Of the Nanocrystalsmentioning

confidence: 99%

“…In comparison with other fluorescent materials such as organic dyes and quantum dots, UCNs exhibit many advantages such as high photochemical stability, sharp emission peaks, large anti-stokes shifts, low toxicity and long lifetime [1][2][3][4][5][6][7][8][9][10][11][12][13]. In addition, high penetration depth and absence of autofluorescence make them ideal materials for biological labeling and in-vivo imaging [14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

“…This work could provide new insight into the preparation of upconversion nanomaterials with strong upconversion luminescence (UCL) and uniform morphology. 3 were prepared by dissolving their corresponding metal oxides in hydrochloric acid at elevated temperature.…”

Section: Introductionmentioning

confidence: 99%

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Preparation of Upconversion Nanocrystals NaYF4:Yb/Tm with Hydro(solvo)thermal Methods

Zhao¹,

Yin²

2017

Nano BioMed ENG

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A upconversion nanocrystal of NaYF 4 :Yb/Tm was synthesized successfully by two different methods of solvothermal and hydrothermal, respectively. The properties of the products were characterized and compared. The results showed that the nanocrystal prepared by hydrothermal method exhibited uniform hexagonal phase and large size, while the nanocrystal prepared by solvothermal method displayed high upconversion luminescence (UCL) and small size. The UCL intensity of the nanocrystal from solvothermal method was higher than that of hydrothermal method. This is the first time to systematically compare the performances of the upconversion nanocrystal prepared by solvothermal and hydrothermal methods, which provides some new insight into the preparation of upconversion nanomaterials with intense UCL and controllable morphology.

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Section: Upconversion Luminescence Properties Of the Nanocrystalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Preparation of Upconversion Nanocrystals NaYF4:Yb/Tm with Hydro(solvo)thermal Methods

Zhao¹,

Yin²

2017

Nano BioMed ENG

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“…2 3 In this process, whether in the need of a rapid diagnosis of infectious diseases, or mass census to the major diseases which have heavy damage on public health, researchers need to deal with multiple gene mutational loci of a large number of samples, which may amount to thousands from patients and the control samples in some cases. [4][5][6] Therefore, it is extremely necessary to develop a detection platform on nucleic acid suited to clinical application with high efficiency, * Author to whom correspondence should be addressed. rapidness, multiplex samples, multiple polymorphous loci and high throughput.…”

Section: Introductionmentioning

confidence: 99%

High Throughput SNP Detection System Based on Magnetic Nanoparticles Separation

Liu

Jia²,

Ma³

et al. 2013

j biomed nanotechnol

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Single-nucleotide polymorphism (SNP) was one-base variations in DNA sequence that can often be helpful to find genes associations for hereditary disease, communicable disease and so on. We developed a high throughput SNP detection system based on magnetic nanoparticles (MNPs) separation and dual-color hybridization or single base extension. This system includes a magnetic separation unit for sample separation, three high precision robot arms for pipetting and microtiter plate transferring respectively, an accurate temperature control unit for PCR and DNA hybridization and a high accurate and sensitive optical signal detection unit for fluorescence detection. The cyclooxygenase-2 gene promoter region −765G > C polymorphism locus SNP genotyping experiment for 48 samples from the northern Jiangsu area has been done to verify that if this system can simplify manual operation of the researchers, save time and improve efficiency in SNP genotyping experiments. It can realize sample preparation, target sequence amplification, signal detection and data analysis automatically and can be used in clinical molecule diagnosis and high throughput fluorescence immunological detection and so on.

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“…In recent years, magnetic nanoparticles have attracted a great deal of interest in various fields of advanced biological and medical applications, such as RNA and DNA purification, immunoassays, cell isolation, and DNA detection. [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42] Based on using magnetic nanoparticles as target DNA carrier, we have developed couple of genotyping methods previously which avoid any complex procedure including purification and concentration, [43][44] however, it still requires two types of labeled probes for each SNP locus, it increase the cost on the reagents when doing multiplexing analysis.…”

Section: Introductionmentioning

confidence: 99%

An Automatic High-Throughput Single Nucleotide Polymorphism Genotyping Approach Based on Universal Tagged Arrays and Magnetic Nanoparticles

Li¹,

Liu²,

Jia³

et al. 2013

Journal of Biomedical Nanotechnology

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Recent developments in highly parallel genome-wide studies are transforming the association of human health and diseases. In these studies, multiple SNP loci from large amount of samples need to be investigated to obtain a result with a high degree of confidence. Herein, we describe a novel, cost-effective and automated method for high-throughput single nucleotide polymorphisms (SNPs) genotyping based on universal tagged array and magnetic separation. By using two kinds of functionalized magnetic nanoparticles, the whole operation procedure including genome DNA extraction and SNP genotyping can be automatically performed by JANUS automated workstation (Perkin Elmer Inc.). Four different SNPs loci from 80 samples were scored using only one pair of universal dual-color probes, the phase of numerous SNPs can be automated assessed simultaneously. The results demonstrated that the expected scores and good discrimination were obtained between the two alleles from these four SNP loci. Due to adequately taking the advantages of high parallel read-out and intrinsically scalable properties of microarray, and the automated magnetic separation handling technology is highly adaptable fro multiplexing sample preparation and automated SNP analysis, also avoid the complex procedure including purification and concentration, the new strategy is high-throughput, simple, flexible, cost-effective, and will be very suitable for large-scale genotyping.

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Chemiluminescence Molecular Detection of Sequence-Specific HBV-DNA Using Magnetic Nanoparticles

Cited by 47 publications

References 4 publications

Preparation of Upconversion Nanocrystals NaYF4:Yb/Tm with Hydro(solvo)thermal Methods

Preparation of Upconversion Nanocrystals NaYF4:Yb/Tm with Hydro(solvo)thermal Methods

High Throughput SNP Detection System Based on Magnetic Nanoparticles Separation

An Automatic High-Throughput Single Nucleotide Polymorphism Genotyping Approach Based on Universal Tagged Arrays and Magnetic Nanoparticles

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