Chemiluminescence yields and detection limits of some isoluminol derivatives in various oxidation systems

Schroeder, Hartmut; Yeager, Frances M.

doi:10.1021/ac50030a027

Cited by 146 publications

(44 citation statements)

References 24 publications

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“…The luminescent label, 9-(4-succinimidobutyl-N-ethyl) aminobenzo (0 phthalazine-1,4 (2H, 3H) dione (ABEN-H), was synthesised according to Schroeder et al (12) and was coupled to antibodies after synthesis of its N-hydroxysuccinimide ester (13). The radioimmunoassay kit used for comparison was the prostate-specific antigen (ProsChek RIA) from Yang Laboratories (1BL, Hamburg, Germany).…”

Section: Methodsmentioning

confidence: 99%

The Establishment and Evaluation of Luminescent-Labelled Immunometric Assays for Prostate-Speciflc Antigen-α1-Antichymotrypsin Complexes in Serum

Wood¹,

Sloot²,

Böhle³

1991

Clinical Chemistry and Laboratory Medicine

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Summary:Prostate-specific antigen is found in the prostate in two forms, one with a low (30 000) and one with a high (100000) relative molecular mass. The latter has recently been found to be a complex of prostatespecific antigen with aj-antichymotrypsin.Immunoluminometric assays were designed for the prostate-specific antigen-ocj-antichymotrypsin complex äs well äs for (Xi-antichymotrypsin, the former being compared with a commercially available radioimmunoassay for prostate-specific antigen (ProsChek RIA -Yang Laboratories).The precision of the immunoluminometric assays was acceptable (intra-assay Variation < 7%; inter-assay Variation < 8.5%) in the measuring ranges 0-90 g/l for the prostate-specific antigen-a r antichymotrypsin complex and 0-12 g/l for a r antichymotrypsin.The correlation between the assays for prostate-specific antigen and prostate-specific antigen-aj-antichymotrypsin complex was acceptable, showing a correlation coefficient r = 0.83 after double logarithmic transformation, or r = 0.85 using the Spearman rank correlation on 131 data pairs.Extremely high arantichymotrypsin levels (above 2 g/l) caused interference in the prostate-specific antigena r antichymötrypsin complex assay. Such levels, although rare, are encountered in pulmonary inflammatory disease. The reference ranges for the three assays were found to be äs follows: prostate-specific antigen 0.13-4.63 g/l, prostate-specific antigeii-ai-antichymotrypsin complex 0.08 -1.78 g/l, and for ai-antichymotrypsin 0.27-0.61 g/l. These values were obtained from 82 höspitalised males fpr the first two assays and from 80 males and females free from infection fpf the latter. % Purified prostate-specific antigen (M r 30000) does not react in the prostate-specific antigen-arantichymotrypsin complex as$ay; a concentration of 200 /1 generates a signal whieh is less than that from the first Standard (0.04 g/l).In three cases of metästatic cancer of the prostate, discrepancies were found in the values from the prostatespecific antigen assay (8.56, 30.0 and 107 g/l) and the prostate-specific antigen-a r antichymotrypsin complex assay (0.78, 3.72 and 1,24 jig/1). This may indieate the production of an altered prostate-specific antigen, which was unable to complex with a r antichymotrypsin.The new assay is not suitable äs a screening assay for prostatic cancer, but it may be of interest for detecting metästatic disease.

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Section: Methodsmentioning

confidence: 99%

The Establishment and Evaluation of Luminescent-Labelled Immunometric Assays for Prostate-Speciflc Antigen-α1-Antichymotrypsin Complexes in Serum

Wood¹,

Sloot²,

Böhle³

1991

Clinical Chemistry and Laboratory Medicine

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“…Both reactions are oxidative and occur in alkaline conditions, though in the case of luminol, there is the absolute requirement for a catalyst. This can be a simple transition metal cation such as Mn ++ or Ni ++ but may also be a complex macromolecule such as horseradish peroxidase and cytochromes (17). The most popular catalyst is microperoxidase since this yields an efficient reaction under relatively mild conditions.…”

Section: Chemiluminescence Immunoassay (Clia)mentioning

confidence: 99%

Chemiluminescence Immunoassay: Basic Mechanism and Applications

Azim

Hasan

Ansari

et al. 2018

Bangladesh J. Nuclear Med.

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Chemiluminescence immunoassay (CLIA) has been widely applied in different fields including environmental monitoring, liquid chromatography, clinical diagnosis; food safety, pharmaceutical analysis, immuno-and gene probe assays, as a promising approach for selective, sensitive, rapid and simple analysis. It is often necessary to detect a large number of complicated or low-abundance samples. However, traditional methods need great consumption of time, reagents and labor, which limit their clinical applications. As a result, rapid, high-throughput, sensitive and low-cost detection methods have become the development trend of CLIA. A new chemiluminescence immunoassay analyzer with advanced acridinium ester (AE) technology will be installed at the radioimmunoassy laboratory of National Institute of Nuclear Medicine and Allied Sciences (NINMAS) shortly. In conjunction with the existing Radioimmunoassay (RIA), Immunoradiometric assay (IRMA) and DissociationEnhanced Lanthanide Fluorescent Immunoassay (DELFIA), introduction of chemiluminescence immunoassay (CLIA) technology will expand the activities of in vitro immunoassay. It will also improve the research and development activities of in vitro division of NINMAS. In this review article, we have summarized the basic principle of chemiluminescence immunoassay and its applications. A comparison of RIA, CLIA and enzyme-linked immunosorbent assay (ELISA) was also briefly described in the article.

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“…Os vários tipos de luminescência são caracterizados em função da fonte de energia empregada para se obter o estado excitado. Na quimiluminescência, a energia de excitação é proporcionada por uma reação química, sendo a emissão de radiação observada geralmente nas regiões do visível ou infravermelho próximo 3,4 . Esse processo pode ser simplificadamente representado pela Equação 1, onde A e B representam os reagentes, AB* o intermediário no estado excitado, AB o produto da reação, hν ν ν ν ν a radiação emitida e C um catalisador.…”

Section: Quimiluminescênciaunclassified

Um experimento de análise em fluxo envolvendo reações enzimáticas e quimiluminescência

Leite¹,

Fatibello‐Filho²,

Rocha³

2004

Quím. Nova

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Recebido em 7/3/03; aceito em 13/8/03A FLOW ANALYSIS EXPERIMENT INVOLVING ENZYMATIC REACTIONS AND CHEMILUMINESCENCE. An experiment is proposed to introduce some fundamentals of flow analysis, chemiluminescence and kinetic monitoring of enzymatic reactions in undergraduate courses. Chemiluminescence detection is performed with a simple spectrophotometer equipped with a lab-made spiral flow cell constructed from a polyethylene tube. The hydrogen peroxide produced by the glucose oxidation in the presence of glucose oxidase is continuously monitored by the reaction with luminol in alkaline media in a flow injection system. The exercise allows also the discussion of important analytical features and the comparison with different optical methods of analysis.Keywords: chemiluminescence; flow analysis; enzymatic reactions. INTRODUÇÃOO desenvolvimento da Química e os trabalhos de interface com áreas correlatas têm gerado grandes desafios para o ensino desta ciên-cia. Um deles é a necessidade de incluir uma quantidade cada vez maior de conceitos, técnicas e informações importantes, sem que haja aumento do tempo dedicado às diferentes disciplinas. Uma alternativa para este problema é a proposição de experimentos que associem diferentes informações, permitindo explorar melhor o tempo disponível e, ao mesmo tempo, reduzir gastos. Essa estratégia também permite demonstrar que a compartimentalização da Química em diferentes áreas é artificial 1 , visto que a interpretação dos fenômenos com que o aluno se depara no dia-a-dia requer uma abordagem diferente, que utilize os conceitos químicos de maneira unificada e associada para seu entendimento. Os diferentes conceitos e técnicas introduzidos nas disciplinas de Química Analítica, por exemplo, são indispensáveis para a obtenção de informações relacionadas às outras disciplinas.Neste contexto, é proposto um experimento para alunos de graduação em Química e áreas correlatas, que permite introduzir conceitos relacionados à análise em fluxo, quimiluminescência, reações enzimáticas e cinética química. A reação de oxidação da glicose na presença de glicose oxidase (GOD) foi estudada através do monitoramento da quimiluminescência da oxidação do luminol pelo peróxido de hidrogênio produzido. Uma cela em espiral construída com tubo de polietileno e adaptada a um espectrofotômetro simples permite efetuar medidas por quimiluminescência com equipamentos usuais em laboratórios de ensino. QUIMILUMINESCÊNCIALuminescência é um termo empregado para descrever a emissão de radiação quando uma molécula ou átomo no estado excitado decai para o seu estado fundamental 2 . Os vários tipos de luminescência são caracterizados em função da fonte de energia empregada para se obter o estado excitado. Na quimiluminescência, a energia de excitação é proporcionada por uma reação química, sendo a emissão de radiação observada geralmente nas regiões do visível ou infravermelho próximo 3,4 . Esse processo pode ser simplificadamente representado pela Equação 1, onde A e B representam os reagentes, AB* o intermediár...

show abstract

Chemiluminescence yields and detection limits of some isoluminol derivatives in various oxidation systems

Cited by 146 publications

References 24 publications

The Establishment and Evaluation of Luminescent-Labelled Immunometric Assays for Prostate-Speciflc Antigen-α1-Antichymotrypsin Complexes in Serum

The Establishment and Evaluation of Luminescent-Labelled Immunometric Assays for Prostate-Speciflc Antigen-α1-Antichymotrypsin Complexes in Serum

Chemiluminescence Immunoassay: Basic Mechanism and Applications

Um experimento de análise em fluxo envolvendo reações enzimáticas e quimiluminescência

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