Chemiluminescent response in murine peritoneal macrophage hybridoma-cell line (2C11-12) primed with lipopolysaccharide or interferon β

Ardekani, Ali M.; Debaetselier, P.; Dyke, Knox Van

doi:10.1016/0026-265x(89)90064-7

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…The inhibition of the light reaction indicates that the enzyme activity responsible for the formation of peroxynitrite may be markedly increased or induced during an inflammatory insult. It is known from earlier studies that the inflammatory mediators gamma interferon and lipopolysaccharide increase luminoldependent chemiluminescence of cultured phagocytic cells (19). There then may be a coordinate induction of nitric oxide synthase and NADPH oxidase in the phagocytic cells and tissues recovered from the lungs of animals treated with silica.…”

Section: Discussionmentioning

confidence: 98%

Introduction of luminol-dependent chemiluminescence as a method to study silica inflammation in the tissue and phagocytic cells of rat lung.

Antonini

Dyke

et al. 1994

Environ Health Perspect

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The inhalation of silica has been shown to produce a dramatic inflammatory and toxic response within the lungs of humans and laboratory animals. A variety of cellular and biochemical parameters are used to assess the silica-induced lung injury. The purpose of this paper is to introduce the use of luminol-dependent chemiluminescence as a new method to study inflammation in both phagocytic cells and lung tissue recovered from silica-exposed animals. Chemiluminescence, or the emission of light, accompanies the release of reactive forms of oxygen when phagocytic cells are challenged. In this study, male Fischer 344 rats were intratracheally instilled with either silica (10 mg/100 g bw) or saline vehicle. One day after the instillations, a marked increase in the chemiluminescence was observed in the lung tissue and bronchoalveolar lavage cells recovered from the silica-treated animals when compared with the saline controls. The light reaction was markedly decreased by either superoxide dismutase of N-nitro-arginine methyl ester hydrochloride. Superoxide dismutase is involved in the enzymatic breakdown of superoxide anion, while N-nitro-L-arginine methyl ester hydrocholoride, a nitric oxide synthase inhibitor, prevents the formation of nitric oxide. When superoxide anion and nitric oxide react, they form the highly oxidizing substance peroxynitrite. This study then implicates peroxynitrite as an agent that may be responsible for some of the oxidant lung injury that is associated with silica exposure. The use of luminol-dependent chemiluminescence may prove valuable as a method to measure the earliest events in the inflammatory process, and may be an adjunct in studying the mechanisms that produce inflammation.

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Section: Discussionmentioning

confidence: 98%

Introduction of luminol-dependent chemiluminescence as a method to study silica inflammation in the tissue and phagocytic cells of rat lung.

Antonini

Dyke

et al. 1994

Environ Health Perspect

View full text Add to dashboard Cite

show abstract

“…The inhibition of the light reaction indicates that the enzyme activity responsible for the formation of peroxynitrite may be markedly increased or induced during an inflammatory insult. It is known from earlier studies that the Volume 102, Supplement 10, December 1994 inflammatory mediators gamma interferon and lipopolysaccharide increase luminoldependent chemiluminescence of cultured phagocytic cells (19). There then may be a coordinate induction of nitric oxide synthase and NADPH oxidase in the phagocytic cells and tissues recovered from the lungs of animals treated with silica.…”

Section: Discussionmentioning

confidence: 99%

Introduction of Luminol-Dependent Chemiluminescence as a Method to Study Silica Inflammation in the Tissue and Phagocytic Cells of Rat Lung

Antonini¹,

Dyke²,

Ye³

et al. 1994

Environmental Health Perspectives

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The inhalation of silica has been shown to produce a dramatic inflammatory and toxic response within the lungs of humans and laboratory animals. A variety of cellular and biochemical parameters are used to assess the silica-induced lung injury. The purpose of this paper is to introduce the use of luminol-dependent chemiluminescence as a new method to study inflammation in both phagocytic cells and lung tissue recovered from silicaexposed animals. Chemiluminescence, or the emission of light, accompanies the release of reactive forms of oxygen when phagocytic cells are challenged. In this study, male Fischer 344 rats were intratracheally instilled with either silica (10 mg/100 g bw) or saline vehicle. One day after the instillations, a marked increase in the chemiluminescence was observed in the lung tissue and bronchoalveolar lavage cells recovered from the silica-treated animals when compared with the saline controls. The light reaction was markedly decreased by either superoxide dismutase or /M-nitro-Larginine methyl ester hydrochloride. Superoxide dismutase is involved in the enzymatic breakdown of superoxide anion, while N-nitro-L-arginine methyl ester hydrocholoride, a nitric oxide synthase inhibitor, prevents the formation of nitric oxide. When superoxide anion and nitric oxide react, they form the highly oxidizing substance peroxynitrite. This study then implicates peroxynitrite as an agent that may be responsible for some of the oxidant lung injury that is associated with silica exposure. The use of luminol-dependent chemiluminescence may prove valuable as a method to measure the earliest events in the inflammatory process, and may be an adjunct in studying the mechanisms that produce inflammation. -Environ Health Perspect 102(Suppl 10): 37-42 (1994)

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Chemiluminescent response in murine peritoneal macrophage hybridoma-cell line (2C11-12) primed with lipopolysaccharide or interferon β

Cited by 2 publications

References 28 publications

Introduction of luminol-dependent chemiluminescence as a method to study silica inflammation in the tissue and phagocytic cells of rat lung.

Introduction of luminol-dependent chemiluminescence as a method to study silica inflammation in the tissue and phagocytic cells of rat lung.

Introduction of Luminol-Dependent Chemiluminescence as a Method to Study Silica Inflammation in the Tissue and Phagocytic Cells of Rat Lung

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