Chemistry of Lipids of Posthemolytic Residue or Stroma of Erythrocytes

Haptenic Relationship between Bovine J, Human A, and Porcine A Blood Group Systems Tested with Lipids and Nonlipid Fractions of Blood

“…In contrast, the lipids and nonlipid fractions prepared from serum of humans with cellular A antigen are not reactive in any of the blood group systems under investigation (tables I and II). It is well established that the cellular ABH-substances of man are bound to lipids [25] ; the soluble ABHsubstances present in the saliva and other secretions of about 80% of indivi duals, however, are mucoproteins [12]. Apart from the activity of human A red cell lipids in the human A system and in the cross-reacting systems (table I), we have also found a similar reactivity of the human A red cell mucoproteins in the same blood group systems (table II).…”

Blood Group Antigens in Relation to Chemical and Structural Properties of the Red Cell Membrane

“…Yamakawa et al [50], Hakomori and Jeanloz [16] Koscielak [23] inde pendently isolated and characterised glycolipid fractions with blood group A and B activity from red cells (Table VII). Although progress has been made recently with regard to purity of these glycolipids [24,51,52,17] sequential and configurational analysis are still lacking. Uhlenbruck [44] proposed a hypothetical structure for blood group A and B active glycolipids; this hypothesis is based on structural information of sialic acid containing gangliosides from brain and on Yamakawa's [51] concept of the structure of globoside I, a tetrahexoside from ROM which exhibits no blood group activity.…”

Partial Purification of ABO Blood Group Antigens: Sodium Deoxycholate Fractionation of Human Erythrocyte Membranes

“…Stellner et al [28] demonstrated ethanol-extracted glycosphingolipids from erythrocyte membranes which showed H activity but lacked Leb activity. A glycosphingolipid fraction prepared from ethanol extracts of A1 erythrocytes contained four different A variants, Aa, Ab, Ac, and Aa, which differed in oligosaccharide structure and in the molar ratios of the constituent mono saccharide components [7], Whittemore [31], utilizing n-butanol as a solvent, demonstrated the solubilization of a glycoprotein from erythrocytes that ex hibited greater ABO blood group activity than the glycolipid preparations of Yamakawa et al [32] and Koscielak [12], Gardas and Koscielak [5] have also isolated erythrocyte membrane glycoproteins which have ABO activity.…”

“…The characterization of similar antigenic ABO substances from erythrocyte membranes has been limited because of the difficulty in solubilizing material from this source. Yamakawa et al [32] and Koscielak [12] have reported that the extrac tion of red cells with organic solvents and subsequent purification on cel lulose and silicic acid columns yields group A or B antigens which are glycolipids and lack protein. In further investigations, Koscielak et al [13] have characterized several fucose-containing glycosphingolipids from ethanol-extracted stroma.…”