Chemopreventive Effect of Curcumin on Oxidative Stress, Antioxidant Status, 
 DNA Fragmentation and Caspase-9 Gene Expression in 1,2-dimethylhydrazine-induced 
 Colon Cancer in Rats

Aziza, Samy Ali Hussein; Abdel-Aal, Samir A.; Mady, Hossam A.

doi:10.3923/ajbmb.2014.22.34

Cited by 12 publications

(8 citation statements)

References 29 publications

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“…In the present study, levels of GSH, and CAT in two groups of S. lavandulifolia users showed a signi cant increase compared to pre-test. MDA showed a signi cant decrease respectively in S. lavandulifolia group, this is consistent with results of several studies (34). Taking the results collectively, treatment with the aerial parts of S. lavandulifolia led to a considerable reduction in oxidative stress.…”

Section: Discussionsupporting

confidence: 91%

Effect of 12 Months of Exercise With Stachys Lavandulifolia Consumption on Anxiety, Metabolic Syndrome Profiles and Antioxidant Defense and Lipid Peroxidation in Women With Metabolic Syndrome

Osali

Rostami

2021

Preprint

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Background: The purpose of this study was to investigate the effect of 12 months of aerobic exercise combining stachys lavandulifolia (S. lavandulifolia) consumption on anxiety, Metabolic Syndrome profiles and antioxidant defense (Glutathione) and lipid peroxidation (Malondialdehyde) in 50-65 years old women with metabolic syndrome.Methods: 48 women with Metabolic syndrome were randomly divided into four groups: exercise (n=12), exercise+S. lavandulifolia (n=12), S. lavandulifolia (n=12) and control group (n=12). S. lavandulifolia groups consumed 3 g aerial parts of S. lavandulifolia daily. Training groups performed an exercise protocol of aerobic exercise for 12 months (three sessions per week). Blood samples were obtained before and after training period for antioxidant indicators and lipid degradation measurement. Also, Beck anxiety questionnaire used for evaluating levels of anxiety. T-test and one-way analysis of variance were used for the evaluation of within-group and between-group differences, respectively.Results: A significant increase was observed in serum levels of Malondialdehyde (P =0.004), Catalase indexes (Pvalue= 0.01), and Glutathione (P=0.001) in the training group and S. lavandulifolia groups after 12 months. Body weight, BMI, and SBP and Anexiety was decreased significantly greater in exercise +S. lavandulifolia group compared to control, exercise and S. lavandulifolia groups (P=0.001)Conclusion: Anxiolytic effect and Anti-Oxidative Stress Activity was seen, so taking S. lavandulifolia along with exercises may have beneficial effects on reinforcement the antioxidant system and prevention of anxiety and The negative effects of indicators related to cardiovascular disease in women with metabolic syndrome.

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Section: Discussionsupporting

confidence: 91%

Effect of 12 Months of Exercise With Stachys Lavandulifolia Consumption on Anxiety, Metabolic Syndrome Profiles and Antioxidant Defense and Lipid Peroxidation in Women With Metabolic Syndrome

Osali

Rostami

2021

Preprint

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“…Earlier studies in 2012, have demonstrated that SOD3 (extracellular copper-zinc SOD) protects against destructive effects which roots from ROS 30,31. In 2014, Aziza reported that curcumin rose the activity of SOD, protecting the enzymatic antioxidants via denaturation 32. Zhu et al (2015) have shown that SOD and GSH levels were significantly higher in the ARPE-19 cells treated with curcumin than in the non-treated ones 21.…”

Section: Discussionmentioning

confidence: 99%

<p>Effect of curcumin on gene expression and protein level of methionine sulfoxide reductase A (MSRA), SOD, CAT and GPx in Freund’s adjuvant inflammation-induced male rats</p>

Meshkibaf¹,

Maleknia²,

Noroozi³

2019

JIR

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Objective Curcumin is the well-known compound which is extracted from turmeric powder, the dried rhizome of the Curcuma longa Linn. This have been used for the treatment of various disorders including inflammation. In this study we have analyzed the effect of curcumin on arthritis induced by adjuvant in rats, considering changes in methionine sulfoxide reductase A (MSRA) expression and antioxidant enzymes levels. Methods Five groups of adult male Wistar rats (n=10), were randomly selected as control, placebo, experimental 1, 2 and 3. The induction of arthritis was carried out by injection of 0.1 ml adjuvant in plantar region. The first experimental group received no curcumin treatment, whereas the experimental two and three received curcumin (1 and 2 g/kg daily) respectively, for fourteen days. MSRA gene expression was assessed by real-time PCR and protein levels of MSRA, SOD, CAT and GPx were analyzed via ELISA method. Results The results showed no significant weight changes among the groups during the experimental period and the paw swelling caused by adjuvant was recovered within fourteen days of treatment with curcumin. However, the levels of enzymes such as superoxide dismutase, catalase and glutathione peroxidase were increased by a dose dependent manner. These results also illustrated that the gene expression and protein level of MSRA in groups treated with curcumin increased significantly ( p ≤0.05). Conclusion We concluded that the curcumin can be used against inflammation. The increasing level of MSRA can be due to the antioxidant effect of curcumin. The enzymatic level changes (MSRA, SOD, CAT and GPx) may interfere with the aging process and delay it.

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“…Our current study also showed a strong resemblance to the previous study, with a significant decrease in CAT activity (p ≤ 0.05) in DMH control compared to a healthy control group, whereas AR with both doses of 5 mg/kg and 10 mg/kg showed a significant increase in CAT activity (p ≤ 0.05). Many studies have found that DMH reduces the activity of antioxidants such as SOD and CAT [ [64] , [65] , [66] , [67] ]. Several studies have also found significantly higher levels of MDA in DMH-treated rats [ 64 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have found that DMH reduces the activity of antioxidants such as SOD and CAT [ [64] , [65] , [66] , [67] ]. Several studies have also found significantly higher levels of MDA in DMH-treated rats [ 64 , 65 ]. In our study, we found that the activity of SOD and CAT was significantly reduced in DMH-treated rats compared to other groups, while MDA activity was significantly higher in DMH-treated colon tissue.…”

Section: Discussionmentioning

confidence: 99%

Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats

Kumar

et al. 2021

Journal of Ayurveda and Integrative Medicine

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Background Colorectal cancer (CC) is the third most common cancer in the world. Annona reticulata (AR) also known as bullock's heart, is a traditional herb. AR leaf extract was initially investigated for its anti-bacterial, anti-inflammatory, anti-malarial, anti-helminthic, anti-stress, and wound healing properties. Only a few in vitro cancer studies have been conducted on AR. Although few studies have linked AR leaf extract to many cancers, comprehensive studies addressing regulation, biological functions, and molecular mechanisms leading to CC pathogenesis are clearly lacking. Objectives The present study aimed to explore the antioxidant and anti-cancer potentials of AR leaf extract in CC. Materials and methods The MTT assay was used to test the anti-proliferative activity of AR leaf extract in vitro on the HCT116 cell line. Qualitative and quantitative phytochemical characterization was carried out using gas chromatography: mass spectrometry (GC–MS). 1,2-dimethylhydrazine (DMH) was used to establish CC model in female Wistar rats. The acute toxicity of AR leaf extract was tested in accordance with OECD guidelines. Aberrant Crypt Foci (ACF) count, organ index, and hematological estimations were used to screen for in vivo anti-cancer potential. The antioxidant activity of colon homogenate was determined. Results The alcoholic leaf extract (IC50, 0.55 μg/ml) was found to be more potent than the aqueous extract. Using GC–MS, a total of 108 compounds were quantified in the alcoholic leaf extract. The LD 50 value was found to be safe at a dose of 98.11 mg/kg of body weight. AR alcoholic leaf extract significantly (p < 0.05) decreased ACF count and normalized colon length/weight ratio. AR leaf extract increased RBC, hemoglobin and platelets levels. The AR alcoholic leaf extract reduced the DMH-induced tumors and significantly (p < 0.05) increased the activity of endogenous antioxidant enzymes such as catalase, reduced glutathione, superoxide dismutase, and decreased the lipid peroxidase activity. AR leaf extract reduced the inflammation caused by DMH and helped to repair the colon's damaged muscle layers. Conclusion Based on the findings from the present study, it can be concluded that the alcoholic leaf extract of AR has antioxidant and anti-proliferative properties and can aid in the prevention of CC development and dysplasia caused by DMH.

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Chemopreventive Effect of Curcumin on Oxidative Stress, Antioxidant Status, DNA Fragmentation and Caspase-9 Gene Expression in 1,2-dimethylhydrazine-induced Colon Cancer in Rats

Cited by 12 publications

References 29 publications

Effect of 12 Months of Exercise With Stachys Lavandulifolia Consumption on Anxiety, Metabolic Syndrome Profiles and Antioxidant Defense and Lipid Peroxidation in Women With Metabolic Syndrome

Effect of 12 Months of Exercise With Stachys Lavandulifolia Consumption on Anxiety, Metabolic Syndrome Profiles and Antioxidant Defense and Lipid Peroxidation in Women With Metabolic Syndrome

<p>Effect of curcumin on gene expression and protein level of methionine sulfoxide reductase A (MSRA), SOD, CAT and GPx in Freund’s adjuvant inflammation-induced male rats</p>

Unveiling antioxidant and anti-cancer potentials of characterized Annona reticulata leaf extract in 1,2-dimethylhydrazine-induced colorectal cancer in Wistar rats

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