Chemopreventive efficacy of Wedelia calendulaceae against 20-methylcholanthrene-induced carcinogenesis in mice

Haldar, Pallab Kanti; Bhattacharya, Sanjib; Dewanjee, Saikat; Mazumder, Upal Kanti

doi:10.1016/j.etap.2010.08.003

Cited by 14 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mitochondrial antioxidant enzyme (CAT, SOD, GST, GPx, G6PD, GR etc.) system serves as a line of cellular defense against oxidative stress [ 41 – 43 ]. On other hand, thiol-based antioxidant (GSH) contributes in cellular defense against oxidative damage caused by free radicals.…”

Section: Discussionmentioning

confidence: 99%

The effects of two common edible herbs, Ipomoea aquatica and Enhydra fluctuans, on cadmium-induced pathophysiology: a focus on oxidative defence and anti-apoptotic mechanism

et al. 2015

Self Cite

View full text Add to dashboard Cite

BackgroundIpomoea aquatica (Convolvulaceae) and Enhydra fluctuans (Asteraceae), two aquatic vegetables, are traditionally used against heavy metal toxicity in traditional medicines in India. The present study aimed to explore the protective role of edible (aqueous) extracts of I. aquatica (AEIA) and E. fluctuans (AEEF) against Cd-intoxication.MethodsThe extracts were chemically standardized by spectroscopic and HPLC analysis. The cytoprotective roles of AEIA and AEEF were measured on mouse hepatocytes. The effect on redox status were measured after incubating the hepatocytes with CdCl2 (30 μM) along with AEIA or AEEF (400 μg/ml). The effects on the expressions of apoptotic signal proteins were estimated. The protective roles of AEIA or AEEF were measured by in vivo assay in mice. Haematological, serum biochemical, tissue redox status, Cd bioaccumulation and histological parameters were evaluated to estimate the protective role of AEIA or AEEF (100 mg/kg) against CdCl2 (4 mg/kg) intoxication.ResultsPhytochemical analysis revealed presence of substantial quantities of phenolics, flavonoids, saponins, carbohydrates and ascorbic acid in AEIA or AEEF. CdCl2 treated murine hepatocytes showed a gradual reduction of cell viability in a concentration dependent manner with an IC50 of ~30 μM. CdCl2 treated hepatocytes exhibited significantly enhanced levels (p < 0.01) of ROS production, lipid peroxidation, protein carbonylation and NADPH oxidase with concomitant depletion (p < 0.01) of antioxidant enzymes and GSH. However, AEIA or AEEF treatment along with CdCl2 significantly restored the aforementioned parameters in murine hepatocytes near to normalcy. Besides, AEIA or AEEF significantly counteracted (p < 0.05–0.01) with ROS mediated alteration of transcription levels of signal proteins viz. Bcl-2, BAD, Cyt-C, Caspases, Fas and Bid. In in vivo bioassay, CdCl2 treatment caused significantly high Cd bioaccumulation and oxidative stress in the liver, kidney, heart, brain and testes in mice. In addition, the haematological and serum biochemical parameters were significantly altered in the CdCl2 treated animals. Simultaneous administration of AEIA or AEEF could significantly restore the tested parameters to the near-normal status.ConclusionThe extracts would offer the overall protective effect via counteracting with Cd mediated oxidative stress and/or promoting the elimination of Cd by chelating.

show abstract

Section: Discussionmentioning

confidence: 99%

The effects of two common edible herbs, Ipomoea aquatica and Enhydra fluctuans, on cadmium-induced pathophysiology: a focus on oxidative defence and anti-apoptotic mechanism

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Usually in cancer chemotherapy, the major problems are myelosuppression and anemia. The anemia encountered in tumor bearing mice is mainly due to reduction in RBC or Hb percentage, and this may occur either due to iron deficiency or due to hemolytic or myelopathic conditions (Haldar et al, 2011). Pharmacotherapy with MECC replenishes the RBC and Hb content to the normal levels.…”

Section: Discussionmentioning

confidence: 99%

“…In certain circumstances serum enzymes can be carcinogenic and may engender hepatoxicity (Dolai et al, 2012b). Elevated levels of serum parameters, i.e., SGPT, SGOT and ALP which are indicative of impaired liver functions can be clearly seen due to hepatotoxicity associated after 9 d of inoculation with EAC cells (Haldar et al, 2011). The analysis of changes in serum total protein in malignancy is in itself a means of studying abnormality in the protein metabolism in this condition.…”

Section: Discussionmentioning

confidence: 99%

Antitumor activity and antioxidant property ofCurcuma caesiaagainst Ehrlich’s ascites carcinoma bearing mice

Karmakar

Dolai

Kumar

et al. 2013

Pharmaceutical Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sphagneticola calendulacea (L.) Pruski ( S. calendulacea ) is a small branched herb locally known as Bhimra or Bhringraj, rich in polyphenols [ 14 , 15 ]. Traditionally plant leaves are used in herbal medicine due to possessing cough-relieving, antipyretic, detoxification, antiphlogistic, anti-microbial, anti-inflammatory, anticancer, antidiabetic, and CNS-depressant activities [ 14 , 15 , 16 , 17 , 18 ]. Previous studies suggest that S. calendulacea is rich in polyphenols and a major source of antioxidants which might have anticancer property [ 12 , 15 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Determination of in vitro antioxidant activity and in vivo antineoplastic effects against Ehrlich ascites carcinoma of methanolic extract of Sphagneticola calendulacea (L.) Pruski

Bari

Islam

et al. 2021

Heliyon

View full text Add to dashboard Cite

Background and aim: Sphagneticola calendulacea (L.) Pruski (S. calendulacea) is a well-known medicinal plant that is widely used in traditional medicine. This study was conducted to evaluate the antioxidant and antineoplastic properties of S. calendulacea leaf. Experimental procedure: Methanol was used as a solvent to prepare leaf extract from the dried leaf of S. calendulacea (MESL). We have evaluated the total phenolic and flavonoid contents of MESL, and using five different assays; we have also evaluated the antioxidant property of MESL. In vivo antineoplastic activity of MESL against Ehrlich ascites carcinoma (EAC) cells in experimental mice was also explored. Results: The phytochemical analysis of MESL exhibited the total phenolic, and total flavonoid contents in the dry extracts were 32.86 mg/gm of gallic acid equivalent and 127.26 mg/gm of catechin equivalent, respectively. Moreover, MESL also showed promising scavenging activity in all assays (IC 50 value !70.5 μg/mL). In the in vivo antineoplastic assay, MESL showed increased EAC cell death at the doses of 50 mg/kg and 100 mg/kg body weight. MESL administration also induced apoptosis of EAC cells, significantly inhibited EAC cell growth, and increased the life span of EAC cell-bearing mice compared to EAC cell-bearing control mice. Conclusions: All findings of this study suggest potential antioxidant and antineoplastic properties of MESL.

show abstract

Chemopreventive efficacy of Wedelia calendulaceae against 20-methylcholanthrene-induced carcinogenesis in mice

Cited by 14 publications

References 26 publications

The effects of two common edible herbs, Ipomoea aquatica and Enhydra fluctuans, on cadmium-induced pathophysiology: a focus on oxidative defence and anti-apoptotic mechanism

The effects of two common edible herbs, Ipomoea aquatica and Enhydra fluctuans, on cadmium-induced pathophysiology: a focus on oxidative defence and anti-apoptotic mechanism

Antitumor activity and antioxidant property ofCurcuma caesiaagainst Ehrlich’s ascites carcinoma bearing mice

Determination of in vitro antioxidant activity and in vivo antineoplastic effects against Ehrlich ascites carcinoma of methanolic extract of Sphagneticola calendulacea (L.) Pruski

Contact Info

Product

Resources

About