Chemopreventive properties of curcumin analogues, hexagamavunone-0 and gamavutone-0, in rat colorectal cancer model

Abstract

Purpose: To examine the chemopreventive activity of curcumin analogues, hexagamavunone-0 (HGV-0) and gamavutone-0 (GVT-0), compared to curcumin in a colorectal cancer model in Wistar rats. Methods: Rats (n = 25) were assigned to one of five groups (n = 5 in each group). Colorectal cancer was induced in the control group with subcutaneous injection of 1,2-dimethylhydrazine (DMH) 60 mg/kg once a week for 15 weeks. In addition to DMH injection, treatment groups were treated with curcumin (20, 40, or 80 mg/kg),20, 40, or 80 mg/kg), and hexagamavunone 20, 40, or 80 mg/kg) orally twice a week for 15 weeks. The number and volume of nodules in the colorectal area were observed after laparatomy. Histopathological analysis was performed using H & E staining and immunohistochemistry with antibodies against adenomatous polyposis coli (APC) and cyclooxygenase 2 (COX-2). Results: All treatments reduced colorectal nodule volume, but only HGV-0 significantly decreased the numbers of nodules compared to DMH controls (p < 0.05). The reduction was 96.1 % with 40 mg/kg HGV-0. Mutated APC expression was inhibited by curcumin, GVT-0, and HGV-0 at a dose of 40 mg/kg, whereas COX-2 expression was mostly inhibited by

WITHDRAWN: The Two Mono-Carbonyl Curcumin Analogs, PGV-1 and CCA-1.1: The Chemopreventive Activity Against DMH-Induced Colorectal Cancer Rat and Proteins Target Candidate Involved

“…Formerly, PGV-1 has been shown anti-tumorigenic activities against leukemia and breast cancer in xenograft mice model [6, 7], but has not been explored in colorectal cancer. While the other curcumin analogs, pentagamavunone-0 (PGV-0), gamavutone-0 (GVT-0), and hexagamavunone-0 (HGV-0), exhibited anti-in ammatory activity in the DMH-colorectal cancer rat model [12]. In this regard, we challenge and continue to explore PGV-1 and CCA-1.1, a targeted and selective candidate for colorectal cancer chemotherapeutic agents through in vitro, in vivo, and bioinformatic exploration.…”

“…Red blood count (RBC) and white blood count (WBC) [6] were measured using the Automatic Hematology Analyzer Sysmex KX-21. The nodules were recorded for analysis using equation 1 [12]. where VN = volume of nodules, NL = nodule length, NW = nodule width.…”