Chemosensitizers of the multixenobiotic resistance in amorphous aggregates (marine snow): etiology of mass killing on the benthos in the Northern Adriatic?

Müller, Werner; Riemer, Siegurd; Kurelec, Branko; Smodlaka, Nenad; Puškarić, Staša; Jagic, Bela; Müller-Niklas, Gerald; Quéric, N.

doi:10.1016/s1382-6689(98)00039-8

Cited by 17 publications

(10 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, all MXR‐related gene products were significantly down‐regulated in digestive gland of both in PST‐ as well as in lipophilic toxin‐MBA positive mussels, as well in samples containing high concentrations of lipophilic toxins. This effect is in good agreement with the reduction of MXR transport efficiency at high toxin concentrations previously reported (Müller et al,; Schroder et al,; Eufemia et al, 2002). Considering that elevated MXR‐related gene expression would allow mussels to protect themselves from xenobiotics (Bard,), inhibition of the MXR response may render these organisms more susceptible to anthropogenic pollutants as well as to further natural toxins.…”

Section: Discussionsupporting

confidence: 92%

“…A further potential target for algal toxins is the MXR phenotype, a system of transporter proteins engaged in xenobiotic extrusion from the cells (Bard). Algal products were reported to be natural modulators for the MXR‐related transporters in marine organisms, and the inhibition of MXR transport by algal products was shown in different aquatic molluscs (Müller et al,; Schroder et al,; Eufemia et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Bioaccumulation of algal toxins and changes in physiological parameters in Mediterranean mussels from the North Adriatic Sea (Italy)

Buratti

Franzellitti

Poletti³

et al. 2011

Environmental Toxicology

View full text Add to dashboard Cite

The Northwestern Adriatic Sea is a commercially important area in aquaculture, accounting for about 90% of the Italian mussel production, and it was subjected to recurring cases of mussel farm closures due to toxic algae poisoning. A spatial and temporal survey of four sites along the North Adriatic Sea coasts of Emilia Romagna (Italy) was undertaken to study the possible impairments of physiological parameters in Mytilus galloprovincialis naturally exposed to algal toxins. The sites were selected as part of the monitoring network for the assessment of algal toxins bioaccumulation by the competent Authority. Samples positive to paralytic shellfish toxins and to lipophilic toxins were detected through the mouse bioassay. Lipophilic toxins were assessed by HPLC. Decreasing yessotoxins (YTX) levels were observed in mussels from June to December, while homo-YTX contents increased concomitantly. Lysosome membrane stability (LMS), glutathione S-transferase and catalase activities, and multixenobiotic resistance (MXR)-related gene expressions were assessed as parameters related to the mussel health status and widely utilized in environmental biomonitoring. Levels of cAMP were also measured, as possibly involved in the algal toxin mechanisms of action. Low LMS values were observed in hemocytes from mussels positive to the mouse bioassay. MXR-related gene expressions were greatly inhibited in mussels positive to the mouse bioassay. Clear correlations were established between increasing homo-YTX contents (and decreasing YTX) and increasing cAMP levels in the tissues. Similarly, significant correlations were established between the increase of homo-YTX and cAMP levels, and the expressions of three MXR-related genes at submaximal toxin concentrations. In conclusion, YTXs may affect mussel physiological parameters, including hemocyte functionality, gene expression and cell signaling.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Bioaccumulation of algal toxins and changes in physiological parameters in Mediterranean mussels from the North Adriatic Sea (Italy)

Buratti

Franzellitti

Poletti³

et al. 2011

Environmental Toxicology

View full text Add to dashboard Cite

show abstract

“…This transporter can act both as a positively and as a negatively regulatory machinery for the natural resistance of aquatic organisms [50]. To mention is here that the P-170 system of marine animals is strongly impaired by toxins formed by biofilm-producing microorganisms, “marine snow”, and results in mass killing of e.g., sponges, mussels, or Anthozoa [51]. …”

Section: Discussionmentioning

confidence: 99%

Potentiation of the Cytotoxic Activity of Copper by Polyphosphate on Biofilm-Producing Bacteria: A Bioinspired Approach

et al. 2012

View full text Add to dashboard Cite

Adhesion and accumulation of organic molecules represent an ecologically and economically massive problem. Adhesion of organic molecules is followed by microorganisms, unicellular organisms and plants together with their secreted soluble and structure-associated byproducts, which damage unprotected surfaces of submerged marine structures, including ship hulls and heat exchangers of power plants. This is termed biofouling. The search for less toxic anti-biofilm strategies has intensified since the ban of efficient and cost-effective anti-fouling paints, enriched with the organotin compound tributyltin, not least because of our finding of the ubiquitous toxic/pro-apoptotic effects displayed by this compound [1]. Our proposed bio-inspired approach for controlling, suppressing and interfluencing the dynamic biofouling complex uses copper as one component in an alternative anti-fouling system. In order to avoid and overcome the potential resistance against copper acquired by microorganisms we are using the biopolymer polyphosphate (polyP) as a further component. Prior to being functionally active, polyP has to be hydrolyzed to ortho-phosphate which in turn can bind to copper and export the toxic compound out of the cell. It is shown here that inhibition of the hydrolysis of polyP by the bisphosphonate DMDP strongly increases the toxic effect of copper towards the biofilm-producing Streptococcus mutans in a synergistic manner. This bisphosphonate not only increases the copper-caused inhibition of cell growth but also of biofilm production by the bacteria. The defensin-related ASABF, a marine toxin produced by the sponge Suberites domuncula, caused only an additive inhibitory effect in combination with copper. We conclude that the new strategy, described here, has a superior anti-biofilm potential and can be considered as a novel principle for developing bio-inspired antifouling compounds, or cocktails of different compounds, in the future.

show abstract

“…After washing the animals, they were kept for 5 min in dye-free water to allow rhodamine B to be released from the mantle cavity. The inhibitory potential of the extracts is expressed as verapamil-equivalents per extract, as described [31,32]. The inhibitory potential of the extracts is expressed as verapamil-equivalents per extract, as described [31,32].…”

Section: Bioaccumulation Activitymentioning

confidence: 99%

“…The inhibitory potential of the extracts is expressed as verapamil-equivalents per extract, as described [31,32].…”

Section: Bioaccumulation Activitymentioning

confidence: 99%

Potential Multidrug Resistance Gene Pohl: An Ecologically Relevant Indicator in Marine Sponges

Krasko¹,

Kurelec²,

Batel³

et al. 2001

Environ Toxicol Chem

Self Cite

View full text Add to dashboard Cite

Sponges are sessile filter feeders found in all aquatic habitats from the tropics to the arctic. Against potential environmental hazards, they are provided with efficient defense systems, e.g., protecting chaperones and/or the P-170/multidrug resistance pump system. Here we report on a further multidrug resistance pathway that is related to the pad one homologue (POH1) mechanism recently identified in humans. It is suggested that proteolysis is involved in the inactivation of xenobiotics by the POH1 system. Two cDNAs were cloned, one from the demosponge Geodia cydonium and a second from the hexactinellid sponge Aphrocallistes vastus. The cDNA from G. cydonium, termed GCPOHL, encodes a deduced polypeptide with a size of 34,591 Da and that from A. vastus, AVPOHL, a protein of a calculated M(r) of 34,282. The two sponge cDNAs are highly similar to each other as well as to the known sequences from fungi (Schizosaccharomyces pombe and Saccharomyces cerevisiae) and other Metazoa (from Schistosoma mansoni to humans). Under controlled laboratory conditions, the expression of the potential multidrug resistance gene POHL is, in G. cydonium, strongly upregulated in response to the toxins staurosporin (20 microM) or taxol (50 microM); the first detectable transcripts appear after 1 d and reach a maximum after 3 to 5 d of incubation. The relevance of the expression pattern of the G. cydonium gene POHL for the assessment of pollution in the field was determined at differently polluted sites in the area around Rovinj (Croatia; Mediterranean Sea, Adriatic Sea). The load of the selected sites was assessed by measuring the potency of XAD-7 concentrates of water samples taken from those places to induce the level of benzo[a]pyrene monooxygenase (BaPMO) in fish and to impair the multidrug resistance (MDR)/P-170 extrusion pump in clams. These field experiments revealed that the levels of inducible BaPMO activity in fish and of the MDR potential by the water concentrates are highly correlated with the level of expression of the potential multidrug resistance gene POHL in G. cydonium. This report demonstrates that the detoxification POH pathway, here mediated by the G. cydonium GCPOHL gene, is an additional marker for the assessment of the environmental load in a given marine area.

show abstract

Chemosensitizers of the multixenobiotic resistance in amorphous aggregates (marine snow): etiology of mass killing on the benthos in the Northern Adriatic?

Cited by 17 publications

References 41 publications

Bioaccumulation of algal toxins and changes in physiological parameters in Mediterranean mussels from the North Adriatic Sea (Italy)

Bioaccumulation of algal toxins and changes in physiological parameters in Mediterranean mussels from the North Adriatic Sea (Italy)

Potentiation of the Cytotoxic Activity of Copper by Polyphosphate on Biofilm-Producing Bacteria: A Bioinspired Approach

Potential Multidrug Resistance Gene Pohl: An Ecologically Relevant Indicator in Marine Sponges

Contact Info

Product

Resources

About