Chemotactic activity of oligopeptides containing an EWS motif on <i>Tetrahymena pyriformis</i>: the effect of amidation of the C‐terminal residue

Kőhidai, László; Bősze, Szilvia; Soós, Pál; Illyés, Eszter; Láng, Orsolya; Mák, Marianna; Sebestyén, Ferenc; Hudecz, Ferenc

doi:10.1002/cbf.1005

Cited by 8 publications

(9 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…serotonin and 5-hydroxyindoleacetic acid -, it is not able to differentiate well between the amino acid hormone and the basic amino acid [3]. This could mean that the hormone receptors developed from the amino acid (nourishment) receptors and could explain why all of the amino-acid and peptide hormones studied have receptors in Tetrahymena [203]. However, there are more important amino acids from the point of view of receptor development as e.g.…”

Section: Unicell-induced Thoughts On the Evolution Of Endocrine Systemmentioning

confidence: 98%

The hormonal system of the unicellular Tetrahymena: A review with evolutionary aspects

Csaba

2012

Acta Microbiologica Et Immunologica Hungarica

View full text Add to dashboard Cite

The unicellular ciliate, Tetrahymena has receptors for hormones of the higher ranked animals, these hormones (e.g. insulin, triiodothyronine, ACTH, histamine, etc.) are also produced by it and it has signal pathways and second messengers for signal transmission. These components are chemically and functionally very similar to that of mammalian ones. The exogenously given hormones regulate different functions, as movement, phagocytosis, chemotaxis, cell growth, secretion, excretion and the cells' own hormone production. The receptors are extremely sensitive, certain hormones are sensed (and response is provoked) at 10-21 M concentration, which makes likely that the function could work by the effect of hormones produced by the Tetrahymena itself. The signal reception is selective, it can differentiate between closely related hormones. The review is listing the hormones produced by the Tetrahymena, the receptors which can receive signals and the signal pathways and second messengers as well, as the known effects of mammalian hormones to the life functions of Tetrahymena. The possible and justified role of hormonal system in the Tetrahymena as a single cell and inside the Tetrahymena population, as a community is discussed. The unicellular hormonal system and mammalian endocrine system are compared and evolutionary conclusions are drawn.

show abstract

Section: Unicell-induced Thoughts On the Evolution Of Endocrine Systemmentioning

confidence: 98%

The hormonal system of the unicellular Tetrahymena: A review with evolutionary aspects

Csaba

2012

Acta Microbiologica Et Immunologica Hungarica

View full text Add to dashboard Cite

show abstract

“…In the case of amino acids, correlations were observed between p K values or solvent‐exposed areas (SEAs) (Kőhidai et al ., ) and chemotactic activity, whereas in proline containing dipeptides, the size of the C‐terminal amino acid was found to be decisive for chemotaxis (Kőhidai et al ., ). Investigation of peptides with an EWS motive showed that the chemotactic ability of these ligands was sensitive to amidation of the C‐terminal residue (Kőhidai et al ., ).…”

Section: Resultsmentioning

confidence: 99%

“…The characterization of cell surface–bound molecules responsible for triggering haptotaxis—an alternative form of chemotaxis where the gradient of chemoattractant develops not in a fluid but on a semisolid phase—opened a new approach in chemotaxis research (Rot, ) and led to the recognition of a new group of molecules expressed in inflammation as chemoattractant ligands. Recent studies proved that peptides corresponding to the SXWS sequence, where X = any amino acid, represent one of the new group mentioned earlier (Kőhidai et al ., ). This sequence is part of the extracellular domain of several cytokine receptors (e.g.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Chemotaxis induced by SXWS tetrapeptides in Tetrahymena—overlapping chemotactic effects of SXWS sequences and their identical amino acids

Láng

Illyés

Menyhárd

et al. 2011

J of Molecular Recognition

Self Cite

View full text Add to dashboard Cite

The chemotactic potential of SXWS peptides and the components of the extracellular domain of cytokine receptors were investigated in Tetrahymena as a functional index of substitution with different amino acids in the position 'X' of the tetrapeptide. Data obtained demonstrate that position X plays a special determining role in the ligand, SEWS and STWS possess extremely strong chemoattractant ability, and aromatic amino acids result in chemorepellent ligands. Diverse effects of structurally related molecules, for example, SNWS-SDWS, demonstrate a highly sensitive discrimination potential in the applied model system. Physicochemical characteristics (hydropathy, residue size, and solvent-exposed area) of the amino acids were correlated with the chemotactic activity. Data obtained by computer-assisted conformation analysis of SXWS peptides and the highly overlapping chemotactic effects of the investigated SXWS peptides as well as the presence of the amino acids in the 'X' position indicate that member 'X' of the SXWS sequence performs a special role in interactions with the chemotaxis receptors in the membrane.

show abstract

“…These results showed that some peptides like SEWS (chemotaxis index = 660%) had a pronounced positive chemotactic potential on T. pyriformis , whereas shorter (EWS) and elongated (WSEWS) sequences rather elicited a negative chemotactic effect. Amidation of the C ‐terminus of the peptides and the quality of amino acid at position X also greatly influenced the chemotactic properties of the peptides (Kőhidai et al, , Illyés et al, ).…”

Section: Introductionmentioning

confidence: 99%

Effect of SXWS/WSXWS peptides on chemotaxis and adhesion of the macrophage‐like cell line J774

Szabó

Láng

Lang

et al. 2015

J of Molecular Recognition

Self Cite

View full text Add to dashboard Cite

WSXWS motif is a conserved amino acid sequence which is present in type I cytokine receptors. This motif that can be found both in the ligand binding chains and signal transducer molecule of the receptors with different amino acids at the position 'X', plays a role in the receptor folding, ligand binding and signal transduction as well. Structural analysis proved that WSEWS motif of IL-6R is located in a highly accessible location in the protein.Structural properties and chemotaxis of a tetrapeptide library with SXWS sequence, where X was the 19 proteinogenic amino acids except cystein were systematically studied earlier. It has been proved that C-terminal amidation and the identity of amino acid 'X' had a pronounced influence on the chemotactic properties, but less of the structure of the peptides. Here we present our findings on the effect of a tetra-and a pentapeptide library with the sequence of SXWS and WSXWS on the chemotaxis and adhesion of J774 murine macrophage cell line. We studied the effect of the presence/absence of N-terminal tryptophan and the different amino acids at the 'X' position on these physiological responses. Results indicated that amino acid 'X' had a marked influence on chemotaxis, adhesion as well as on proliferation induced by (W)SXWS peptides. Elongation of SXWS sequence with a tryptophan at the N terminus also altered pronouncedly all the physiological responses of the cells studied. A good correlation could be observed between the chemotaxis and the proliferation and physicochemical parameters of the amino acid 'X'.2

show abstract

Chemotactic activity of oligopeptides containing an EWS motif on Tetrahymena pyriformis: the effect of amidation of the C‐terminal residue

Cited by 8 publications

References 30 publications

The hormonal system of the unicellular Tetrahymena: A review with evolutionary aspects

The hormonal system of the unicellular Tetrahymena: A review with evolutionary aspects

Chemotaxis induced by SXWS tetrapeptides in Tetrahymena—overlapping chemotactic effects of SXWS sequences and their identical amino acids

Effect of SXWS/WSXWS peptides on chemotaxis and adhesion of the macrophage‐like cell line J774

Contact Info

Product

Resources

About