Chemotactic Migration of Human Mesenchymal Stem Cells and MC3T3-E1 Osteoblast-Like Cells Induced by COS-7 Cell Line Expressing rhBMP-7

Lee, Dong Hee; Park, Bong Joo; Lee, Min Sub; Lee, Jin Woo; Kim, Jeong Koo; Yang, Hongmei; Park, Jong Chul

doi:10.1089/ten.2006.12.1577

Cited by 41 publications

(20 citation statements)

References 25 publications

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“…We propose antagonistic roles for BMP7 and BMP2, during feather primordium formation. BMP7 appears to act as a chemoattractant factor for dermal fibroblasts, this effect of BMP7 has also been described on human mesenchymal stem cells (Lee et al, 2006). BMP2, which is expressed later, appears to arrest the migration, slowing down cell recruitment to the condensation.…”

Section: Discussionmentioning

confidence: 83%

BMP2 and BMP7 play antagonistic roles in feather induction

et al. 2008

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Feathers, like hairs, first appear as primordia consisting of an epidermal placode associated with a dermal condensation that is necessary for the continuation of their differentiation. Previously, the BMPs have been proposed to inhibit skin appendage formation. We show that the function of specific BMPs during feather development is more complex. BMP2 and BMP7, which are expressed in both the epidermis and the dermis, are involved in an antagonistic fashion in regulating the formation of dermal condensations, and thus are both necessary for subsequent feather morphogenesis. BMP7 is expressed earlier and functions as a chemoattractant that recruits cells into the condensation, whereas BMP2 is expressed later, and leads to an arrest of cell migration, likely via its modulation of the EIIIA fibronectin domain and α4 integrin expression. Based on the observed cell proliferation, chemotaxis and the timing of BMP2 and BMP7 expression, we propose a mathematical model, a reaction-diffusion system, which not only simulates feather patterning, but which also can account for the negative effects of excess BMP2 or BMP7 on feather formation.

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Section: Discussionmentioning

confidence: 83%

BMP2 and BMP7 play antagonistic roles in feather induction

et al. 2008

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“…To our knowledge, a role of Bmp7 in the regulation of neuronal migration has not been reported before. However, BMP7-mediated chemotactic and inhibitory functions in cell migration have been reported in other systems, including melanomas, osteoblasts and monocytic cell lines (Lee et al, 2006;Na et al, 2009;Perron and Dodd, 2009). …”

Section: Discussionmentioning

confidence: 99%

Appropriate Bmp7 levels are required for the differentiation of midline guidepost cells involved in corpus callosum formation

Sánchez-Camacho

Ortega

Ocaña

et al. 2011

Developmental Neurobiology

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Guidepost cells are essential structures for the establishment of major axonal tracts. How these structures are specified and acquire their axon guidance properties is still poorly understood. Here, we show that in mouse embryos appropriate levels of Bone Morphogenetic Protein 7 (Bmp7), a member of the TGF-β superfamily of secreted proteins, are required for the correct development of the glial wedge, the indusium griseum, and the subcallosal sling, three groups of cells that act as guidepost cells for growing callosal axons. Bmp7 is expressed in the region occupied by these structures and its genetic inactivation in mouse embryos caused a marked reduction and disorganization of these cell populations. On the contrary, infusion of recombinant Bmp7 in the developing forebrain induced their premature differentiation. In both cases, changes were associated with the disruption of callosal axon growth and, in most animals fibers did not cross the midline forming typical Probst bundles. Addition of Bmp7 to cortical explants did not modify the extent of their outgrowth nor their directionality, when explants were exposed to a focalized source of the protein. Together, these results indicate that Bmp7 is indirectly required for corpus callosum formation by controlling the timely differentiation of its guidepost cells.

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“…Bone morphogenetic proteins (BMPs) may be a method by which osteogenesis can be enhanced in a tissue-engineered bone construct since these growth factors are known to promote osseous repair in endochondral and membranous bones. [12][13][14] BMPs are critical for successful fracture repair and bone development, and exogenous delivery of BMP can induce bone formation in critical-sized bone defects. 15,16 Thus, genetic manipulation of cell-seeded MSC constructs with BMP may represent a viable method to accelerate or augment bone deposition.…”

Section: Introductionmentioning

confidence: 99%