Chemotactic selection of Tetrahymena pyriformis GL induced with histamine, di-iodotyrosine or insulin

Kőhidai, László; Schiess, Nicoline; Csaba, G

doi:10.1016/s0742-8413(00)00088-8

Cited by 12 publications

(10 citation statements)

References 23 publications

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“…In protozoon Tetrahymena, various hormone molecules cause a series of special effects. These biologically active molecules can induce and modify the activity of physiological processes such as growth (Christensen, 1993;Christensen et al, 1996aChristensen et al, ,b, 1998Csaba et al, 1985Csaba et al, , 1995Hegyesi and Csaba, 1997;Kristiansen et al, 1996;Shemarova et al, 2002), phagocytosis (Darvas and Csaba, 1990;Ko¨hidai et al, 1995;Kovacs et al, 1996;Schiess et al, 2001), metabolic pathways (Csaba and Lantos, 1976;Csaba and Nagy, 1976;Csaba and Ubornyak, 1982;Ko¨hidai and Csaba, 1985), movement (Csaba and Kovacs, 1993;Ko¨hidai et al, 1994Ko¨hidai et al, , 2000Ko¨hidai et al, , 2002Kovacs et al, 1994;Mugnaini et al, 1995), and secretion activity (Bencsath et al, 1994;Kovacs et al, 1992Kovacs et al, , 1997. Peptide type hormones causing these physiological effects are universal in multicellular systems, but many of them are present in unicellular organisms as well (Csaba and Kovacs, 2000;Csaba et al, 1999;De Pablo et al, 1986;Fulop and Csaba, 1991;Leroith et al, 1980Leroith et al, , 1981Leroith et al, , 1985Schwabe et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Effect of insulin on in vitro fermentation activity of microrganism community of rumen ciliate culture

Kišidayová

Váradyová

2005

Cell Biology International

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The influence of insulin (17.4 nmol l-1) on total gas and methane production, the concentration of total and individual fatty acids and dry matter degradability was investigated in the rumen ciliate culture of Entodinium caudatum. The experimental groups consisted of control group (without insulin) and two groups with insulin application--single shot and long-term application (over 30 days). Fermentation activity of each experimental group was observed on two subgroups: whole protozoan culture (protozoa plus bacteria) and bacterial fraction (bacteria without protozoa). Long-term application of insulin significantly increased methane production and DM degradability in the whole protozoan culture. Total VFA concentration was significantly increased by long-term as well as single-dose application of insulin (by 255% and 158%, respectively). The growth of the protozoa was not influenced by insulin treatments. It can be concluded that the fermentation activity of the community of the rumen ciliate Entodinium caudatum culture was marked stimulated by application of insulin.

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Section: Introductionmentioning

confidence: 99%

Effect of insulin on in vitro fermentation activity of microrganism community of rumen ciliate culture

Kišidayová

Váradyová

2005

Cell Biology International

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“… Effectiveness of responsiveness of cells was calculated according to the following formula: Ch sel =

\begin{matrix} left \\ S / S \cdot C / C \\ \overset{true¯}{S / C \cdot C / S} \end{matrix}

(Kőhidai et al ., ). Values of hydrophobicity were calculated according to Csaba ().…”

Section: Resultsmentioning

confidence: 97%

“…Some chemotactic ligands, for example, vasoactive peptides, are acting on short-term receptors, which are inducible only at the time of exposition (perhaps the ligand itself induces the assembly of the receptor membrane components) (Kőhidai et al, 2001). The long-term receptors are expressed as constitutive functional units of the membrane and are responsible for enhanced chemotactic behavior of subpopulations obtained by chemotactic selection (Kőhidai et al, 2000). In view of these findings, we also studied the relationship between chemotactic selection and the identity of 'X' amino acid in SXWS.…”

Section: Introductionmentioning

confidence: 99%

Chemotaxis induced by SXWS tetrapeptides in Tetrahymena—overlapping chemotactic effects of SXWS sequences and their identical amino acids

Láng

Illyés

Menyhárd

et al. 2011

J of Molecular Recognition

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The chemotactic potential of SXWS peptides and the components of the extracellular domain of cytokine receptors were investigated in Tetrahymena as a functional index of substitution with different amino acids in the position 'X' of the tetrapeptide. Data obtained demonstrate that position X plays a special determining role in the ligand, SEWS and STWS possess extremely strong chemoattractant ability, and aromatic amino acids result in chemorepellent ligands. Diverse effects of structurally related molecules, for example, SNWS-SDWS, demonstrate a highly sensitive discrimination potential in the applied model system. Physicochemical characteristics (hydropathy, residue size, and solvent-exposed area) of the amino acids were correlated with the chemotactic activity. Data obtained by computer-assisted conformation analysis of SXWS peptides and the highly overlapping chemotactic effects of the investigated SXWS peptides as well as the presence of the amino acids in the 'X' position indicate that member 'X' of the SXWS sequence performs a special role in interactions with the chemotaxis receptors in the membrane.

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“…Control samples were evaluated in parallel in each case to eliminate any disturbances consequent on spontaneous mutations. The incubation time was 20 min; its relative brevity facilitated the measurement of purely gradient-directed chemotactic responses and prevented contamination from randomly-running chemokinetic responder cells (KThidai et al, 2000). The samples were fixed in 4% formaldehyde containing PBS and the number of positively-responding cells was counted occulometrically using a Neubauer hemocytometer.…”

Section: Chemotaxis Assaymentioning

confidence: 99%

Effect of tuftsin and oligotuftsins on chemotaxis and chemotactic selection in Tetrahymena pyriformis

Láng

Mező

Hudecz

et al. 2006

Cell Biology International

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The chemotactic properties of tuftsin (H-TKPR-OH), tuftsin derivatives (H-KPR-OH, H-TKPKG-NH(2), Ac-TKPKG-NH(2)) and TKPKG-based oligotuftsins (T20, T30, T40) were investigated in Tetrahymena pyriformis GL. In contrast to its effects on Mammalia, tuftsin elicited chemorepellent or neutral responses; truncation of the N-terminal part (KPR) led to similar results, though with more neutral effects. The significance of the C-terminal part of the molecule was revealed by the chemoattractant properties of TKPKG, which are nevertheless abolished by acylation. Among the oligotuftsins, T20 and T40 were chemoattractants at higher concentrations (10(-9)-10(-6) M), while T30 had a wide-ranging chemorepellent effect, indicating that chemotaxis is elicited in Tetrahymena only by ligands with optimal physicochemical characters (mass, conformation, etc.). The chemotactic selection data indicated that tuftsin-induced chemotaxis results from fairly short-term signalling in Tetrahymena.

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Chemotactic selection of Tetrahymena pyriformis GL induced with histamine, di-iodotyrosine or insulin

Cited by 12 publications

References 23 publications

Effect of insulin on in vitro fermentation activity of microrganism community of rumen ciliate culture

Effect of insulin on in vitro fermentation activity of microrganism community of rumen ciliate culture

Chemotaxis induced by SXWS tetrapeptides in Tetrahymena—overlapping chemotactic effects of SXWS sequences and their identical amino acids

Effect of tuftsin and oligotuftsins on chemotaxis and chemotactic selection in Tetrahymena pyriformis

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