Chicken erythrocytes respond to Toll-like receptor ligands by up-regulating cytokine transcripts

Paul, Michael St.; Paolucci, Sarah; Barjesteh, Neda; Wood, Richard D.; Sharif, Shayan

doi:10.1016/j.rvsc.2013.01.024

Cited by 59 publications

(38 citation statements)

References 23 publications

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“…The excess of erythrocyte transcripts in our data allows us to consider their participation in the immune response, as suggested from some in vitro studies [23, 84]. In most vertebrate species the principal component of blood, erythrocytes, are nucleated, expressing proteins and mRNAs related to various physiological processes other than oxygen transport [85].…”

Section: Discussionmentioning

confidence: 97%

“…Although it has been long known that non-nucleated erythrocytes participate in an immune response [86], the issue is not well studied [85]. Only recently it has been suggested that chicken red blood cells upregulate toll-like receptor 3 (TLR3), type I interferon's (IFN) and IL8 transcripts in response to viral dsRNA mimetic poly I:C [23, 84]. In rainbow trout ( Oncorhynchus mykiss ) erythrocytes, both heat stress and in vitro incubation with LPS modulate genes related to stress, immune response, apoptosis and hematopoiesis [85, 87].…”

Section: Discussionmentioning

confidence: 99%

“…In rainbow trout ( Oncorhynchus mykiss ) erythrocytes, both heat stress and in vitro incubation with LPS modulate genes related to stress, immune response, apoptosis and hematopoiesis [85, 87]. Although upregulation of IL8 coincides with induced expression in chicken erythrocytes by poly I:C [23], no common transcripts with in vitro LPS stimulated rainbow trout erythrocytes [85] could be detected. Our data thus suggest that the role of nucleated erythrocytes in LPS-induced immune response in vivo is small.…”

Section: Discussionmentioning

confidence: 99%

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Profile of whole blood gene expression following immune stimulation in a wild passerine

2014

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BackgroundImmunoecology aims to explain variation among hosts in the strength and efficacy of immunological defences in natural populations. This requires development of biomarkers of the activation of the immune system so that they can be collected non-lethally and sampled from small amounts of easily obtainable tissue. We used transcriptome profiling in wild greenfinches (Carduelis chloris) to detect whole blood transcripts that most profoundly indicate upregulation of antimicrobial defences during acute phase response. The more general aim of this study was to obtain a functional annotation of a substantial portion of the greenfinch transcriptome that would enable to gain access to more specific genomic tools in subsequent studies. The birds received either bacterial lipopolysaccharide or saline injections and RNA-seq transcriptional profiling was performed 12 h after treatment to provide initial functional annotation of the transcriptome and assess whole blood response to immune stimulation.ResultsA total of 66,084 transcripts were obtained from de novo Trinty assembly, out of which 23,153 could be functionally annotated. Only 1,911 of these were significantly upregulated or downregulated. The manipulation caused marked upregulation of several transcripts related to immune activation. These included avian-specific antimicrobial agents avidin and gallinacin, but also some more general host response genes, such as serum amyloid A protein, lymphocyte antigen 75 and copper-transporting ATPase 1. However, links with avian immunity for most differentially regulated transcripts remained rather hypothetical, as a large set of differentially expressed transcripts lacked functional annotation.ConclusionsThis appears to be the first large scale transcriptional profiling of immune function in passerine birds. The transcriptomic data obtained suggest novel markers for the assessment of the immunological state of wild passerines. Characterizing the function of those possible novel infection markers would assist future vertebrate genome annotation. The extensive sequence information collected enables to identify possible target and housekeeping genes needed to gain access to more specific genomic tools in future studies.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-533) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Profile of whole blood gene expression following immune stimulation in a wild passerine

2014

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“…Intriguingly, nucleated erythrocytes from non-mammalian vertebrates such as fish and birds express and regulate specific pattern recognition receptors (PRR), including members of toll-like receptors (TLRs) and peptidoglycan recognition protein (PGRP) receptor families, and are also capable of specific pathogen-associated molecular pattern (PAMP) recognition that is instrumental to the innate immune response (13–15). Furthermore, it has been shown that erythrocytes constitutively express transcripts for different TLRs and respond to TLR ligands by up-regulating type I IFN, IL-8, CCL-4, as well as nitrite production in non-mammalian vertebrates (13, 15).…”

Section: Nucleated Erythrocytes In Vertebratesmentioning

confidence: 99%

New Insight into an Old Concept: Role of Immature Erythroid Cells in Immune Pathogenesis of Neonatal Infection

Elahi

2014

Front. Immunol.

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Newborns are exceedingly susceptible to infection. However, very little is known about what governs the immunological differences seen in early life that result in extreme vulnerability to infection, nor how this changes during infancy. Herein, I provide evidence that the reduced ability to mount a protective immune response to pathogens is not due to an inherent immaturity of neonatal immune cells but instead the functions of these immune cells are actively suppressed by CD71+ erythroid cells. Furthermore, the role of CD71+ erythroid cells in host defense against infection is examined. CD71+ erythroid cells are enriched in newborns and have distinctive immunosuppressive properties that leave them vulnerable to infection. Moreover, immature erythroid cells possess exclusive immunomodulatory properties and may play a role in immune ontogeny. In addition to these distinct features, CD71+ erythroid cells impact digestive health by preventing excessive inflammation following the sudden transition from a sterile in utero setting to excessive colonization with commensals in the external environment. Ongoing research in identifying the beneficial and/or detrimental effects of immature erythrocytes on immune responses may serve to enhance protective newborn immune responses to infection and enable better vaccination strategies for the young to be designed.

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“…This is because studies had documented that flavonoids and phenolic acids act as antioxidants to maintain strong T and B lymphocytes (phagocytes) and reduce vulnerability of the body to infectious diseases as well as preventing angiogenesis in cancer cells (Burchi et al., 2011; Tanya, Xavier, & Clifford, 2014). Centrophils produced from pluripotent hemopoietic stem cells further suggest that MCF‐MORS provokes the production of growth factors (granulocyte‐colony‐stimulating factor [G‐CSF], granulocyte‐macrophage‐colony stimulating factor [GM‐CSF], interleukin 3 and macrophage‐colony‐stimulating factor [M‐CSF]) better than UF‐MOS; as such, prolonged the survival of T‐lymphocytes and natural killer cells (NKs) (Mendy, Yonglian, & Yang‐Xin, 2009) by upregulating the immune response via cytokine release (Jamila, Bharti, & Kanti, 2003; Michael, Sarah, Neda, Wood, & Shayan, 2013). Moreover, recent study had reported that eosinophils possess substantial pro‐inflammatory and cytotoxic activity and responsible for the pathogenesis of various disease processes (Ilesanmi et al., 2016).…”

Section: Resultsmentioning

confidence: 99%

Effect of dietary supplement from mono‐culture fermentation of Moringa oleifera seeds by Rhizopus stolonifer on hematology and markers linked to hypercholesterolemia in rat model

Adedayo

Akintunde

Sani

et al. 2018

Food Science & Nutrition

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Mono‐culture fermentation by Rhizopus stolonifer could promote the healthiness of immune systems and cholesterol levels. Hence, we examined the effect of diet from mono‐culture fermentation of Moringa oleifera seeds by R. stolonifer (MCF‐MORS) on hematological parameters and fundamental indicators of hypercholesterolemia in rat. The animals were divided into six groups (n = 6). Group 1 was placed on basal diet. Group II, III, IV and V were placed on a basal diets supplemented with 7.5%, 15%, 22.5% and 30%, respectively, of MCF‐MORS. Group VI was placed on basal diet fed with unfermented M. oleifera seeds (UF‐MOS). The experiment lasted for eight weeks. The results revealed 7.5% MCF‐MORS as better biological method to augment PCV, RBC and Hb count in animal model. Also, 7.5% and/or 15% MCF‐MORS demonstrated highest levels in centrophils, neutrophils and eosinophils, whereas the levels of lymphocytes, basophils and monocytes showed no significant difference. Similarly, 7.5% and 15% MCF‐MORS modulated LDL and HDL, respectively, better than UF‐MOS; but showing no difference in cholesterol level. MCF‐MORS also maintained architectural integrity of villi and splenocytes better than UF‐MOS. We therefore concluded that diet from MCF‐MORS at 7.5% and 15% modulates HDL, LDL, cholesterol and immune system‐related disorders better than UF‐MOS in rat model.

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Chicken erythrocytes respond to Toll-like receptor ligands by up-regulating cytokine transcripts

Cited by 59 publications

References 23 publications

Profile of whole blood gene expression following immune stimulation in a wild passerine

Profile of whole blood gene expression following immune stimulation in a wild passerine

New Insight into an Old Concept: Role of Immature Erythroid Cells in Immune Pathogenesis of Neonatal Infection

Effect of dietary supplement from mono‐culture fermentation of Moringa oleifera seeds by Rhizopus stolonifer on hematology and markers linked to hypercholesterolemia in rat model

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