Effect of dietary supplement from mono‐culture fermentation of Moringa oleifera seeds by Rhizopus stolonifer on hematology and markers linked to hypercholesterolemia in rat model

Abstract

Mono‐culture fermentation by Rhizopus stolonifer could promote the healthiness of immune systems and cholesterol levels. Hence, we examined the effect of diet from mono‐culture fermentation of Moringa oleifera seeds by R. stolonifer (MCF‐MORS) on hematological parameters and fundamental indicators of hypercholesterolemia in rat. The animals were divided into six groups (n = 6). Group 1 was placed on basal diet. Group II, III, IV and V were placed on a basal diets supplemented with 7.5%, 15%, 22.5% and 30%, respectively, of MCF‐MORS. Group VI was placed on basal diet fed with unfermented M. oleifera seeds (UF‐MOS). The experiment lasted for eight weeks. The results revealed 7.5% MCF‐MORS as better biological method to augment PCV, RBC and Hb count in animal model. Also, 7.5% and/or 15% MCF‐MORS demonstrated highest levels in centrophils, neutrophils and eosinophils, whereas the levels of lymphocytes, basophils and monocytes showed no significant difference. Similarly, 7.5% and 15% MCF‐MORS modulated LDL and HDL, respectively, better than UF‐MOS; but showing no difference in cholesterol level. MCF‐MORS also maintained architectural integrity of villi and splenocytes better than UF‐MOS. We therefore concluded that diet from MCF‐MORS at 7.5% and 15% modulates HDL, LDL, cholesterol and immune system‐related disorders better than UF‐MOS in rat model.

Gentianella acuta mitigates cardiovascular damage and inflammation in diet‑induced hypercholesterolaemic rats

“…These histopathological changes in the body were important formative indices of hypercholesterolaemia, with some beneficial changes appearing in the Model-G group, such as improved aortic wall structure and neatly arranged myocardial cells. Lipid deposition has been identified as an important cause of AS, which can lead to the increase of free radicals, thereby damaging endothelial cell function (30)(31)(32). Thus, the release of protective factors is reduced, leading to a reduction in the tightness of endothelial cells and increased permeability, which in turn results in increased lipid deposition, forming a vicious circle (33).…”