Chicken mannose-binding lectin (MBL) gene variants with influence on MBL serum concentrations

Kjærup, Rikke M.; Norup, Liselotte R.; Skjødt, Karsten; Dalgaard, Tina Sørensen; Juul‐Madsen, Helle R.

doi:10.1007/s00251-013-0689-6

Cited by 16 publications

(16 citation statements)

References 52 publications

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“…MBL and MHC genotype determination MBL genotypes were determined by means of the Kompetitive Allele-Specific PCR (KASP) genotyping technology (LGC Genomics, Hoddesdon, United Kingdom). Using the LGC Genomics KASP assay design service, two assays were developed to distinguish between the CG and TA alleles of SNP1 and the GGGG and AGGA alleles of SNP2 in the MBL promoter region (37). The KASP genotyping technology is based on fluorescently labeled allelespecific PCR primers.…”

Section: Rna and Dna Isolationmentioning

confidence: 99%

Characterization of Cellular and Humoral Immune Responses After IBV Infection in Chicken Lines Differing in MBL Serum Concentration

et al. 2014

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Chickens from two inbred lines selected for high (L10H) or low (L10L) mannose-binding lectin (MBL) serum concentrations were infected with infectious bronchitis virus (IBV), and innate as well as adaptive immunological parameters were measured throughout the experimental period. Chickens with high MBL serum concentrations were found to have less viral load in the trachea than chickens with low MBL serum concentrations indicating that these chickens were less severely affected by the infection. This study is the first to show that MBL expression is present in the lungs of healthy chickens and that the expression is upregulated at days 3 postinfection (p.i.) in L10H chickens. Furthermore, in the liver of infected chickens, the MBL expression was upregulated at day 7 p.i., despite the fact that the MBL serum concentrations were decreased below baseline at that time point. The number of TCRcd + CD8a + cells in the blood of noninfected chickens increased from week 0 to 3 p.i. However, the number of cells was higher in L10H chickens than in L10L chickens throughout the experiment. No increase was observed in the number of TCRcd + CD8a + cells in the blood of the infected L10H and L10L chickens. The numbers of B cells at week 3 p.i. were higher for noninfected L10L chickens than for the other chickens. No differences were observed between the infected and noninfected L10H chickens or between the infected L10H and L10L chickens. Furthermore, at week 3 p.i., the number of monocytes was higher in infected and noninfected L10H chickens than in the infected and noninfected L10L chickens. Thus, these results indicate that MBL is produced locally and may be involved in the regulation of the cellular immune response after an IBV infection. However, MBL did not appear to influence the humoral immune response after IBV infection in this study.

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Section: Rna and Dna Isolationmentioning

confidence: 99%

Characterization of Cellular and Humoral Immune Responses After IBV Infection in Chicken Lines Differing in MBL Serum Concentration

et al. 2014

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“…Two assays were designed, using the Custom TaqMan ® Assay Design Tool according to the instructions on the website (https://www5.invitrogen.com/custom-genomic-products/tools/ genotyping/). These assays distinguish between the CG and TA alleles of SNP1 and the GGGG and AGGA alleles of SNP2 (Kjaerup et al 2013). The assays were validated and run on 384-well microtitre plates in an ABI Prism 7900HT Sequence Detection System instrument, using version 2.2 of the SDS software.…”

Section: Mbl Haplotype Determinationmentioning

confidence: 99%

“…Chickens were tested for their MBL haplotype as described by Kjaerup et al (2013) and were determined through the TaqMan ® SNP genotyping technique (data not shown). All L10L chickens were homozygous for the A1 haplotype.…”

Section: Determination Of Mbl Haplotypesmentioning

confidence: 99%

“…Beside sugars, MBL has also been found to bind to phospholipids, nucleic acids, and non-glycosylated proteins (Ip et al 2009). The MBL genes in human (Madsen et al 1994(Madsen et al , 1995Steffensen et al 2000;Sumiya et al 1991), porcine (Juul-Madsen et al 2011a;Lillie et al 2007), bovine (Liu et al 2011) and chickens (Kjaerup et al 2013) have been found to have polymorphisms resulting in wide variations in MBL serum levels in the organisms.…”

Section: Introductionmentioning

confidence: 99%

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Adjuvant effects of mannose-binding lectin ligands on the immune response to infectious bronchitis vaccine in chickens with high or low serum mannose-binding lectin concentrations

et al. 2014

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Mannose-binding lectin (MBL) plays a major role in the immune response as a soluble pattern-recognition receptor. MBL deficiency and susceptibility to different types of infections have been subject to extensive studies over the last decades. In humans and chickens, several studies have shown that MBL participates in the protection of hosts against virus infections. Infectious bronchitis (IB) is a highly contagious disease of economic importance in the poultry industry caused by the coronavirus infectious bronchitis virus (IBV). MBL has earlier been described to play a potential role in the pathogenesis of IBV infection and the production of IBV-specific antibodies, which may be exploited in optimising IBV vaccine strategies. The present study shows that MBL has the capability to bind to IBV in vitro. Chickens from two inbred lines (L10H and L10L) selected for high or low MBL serum concentrations, respectively, were vaccinated against IBV with or without the addition of the MBL ligands mannan, chitosan and fructooligosaccharide (FOS). The addition of MBL ligands to the IBV vaccine, especially FOS, enhanced the production of IBV-specific IgG antibody production in L10H chickens, but not L10L chickens after the second vaccination. The addition of FOS to the vaccine also increased the number of circulating CD4+ cells in L10H chickens compared to L10L chickens. The L10H chickens as well as the L10L chickens also showed an increased number of CD4-CD8α-γδ T-cells when an MBL ligand was added to the vaccine, most pronouncedly after the first vaccination. As MBL ligands co-administered with IBV vaccine induced differences between the two chicken lines, these results indirectly suggest that MBL is involved in the immune response to IBV vaccination. Furthermore, the higher antibody response in L10H chickens receiving vaccine and FOS makes FOS a potential adjuvant candidate in an IBV vaccine.

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“…This discrepancy may be the result of more stringent requirements in our study (including exclusion of trans binding sites and sites outside of conserved sequences), or potentially due to differing prediction algorithms. HNF3alpha (also known as FOXA1) is known to regulate the expression of human and chicken MBL2 (Naito et al 1999; Kjærup et al 2013), and while binding sites were predicted in CL43 and COLEC11 , no binding site was predicted for bovine MBL2 .…”

Section: Discussionmentioning

confidence: 99%

Identification of polymorphisms in the bovine collagenous lectins and their association with infectious diseases in cattle

2018

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Infectious diseases are a significant issue in animal production systems, including both the dairy and beef cattle industries. Understanding and defining the genetics of infectious disease susceptibility in cattle is an important step in the mitigation of their impact. Collagenous lectins are soluble pattern recognition receptors that form an important part of the innate immune system, which serves as the first line of host defense against pathogens. Polymorphisms in the collagenous lectin genes have been shown in previous studies to contribute to infectious disease susceptibility, and in cattle, mutations in two collagenous lectin genes (MBL1 and MBL2) are associated with mastitis. To further characterize the contribution of variation in the bovine collagenous lectins to infectious disease susceptibility, we used a pooled NGS approach to identify short nucleotide variants (SNVs) in the collagenous lectins (and regulatory DNA) of cattle with (n = 80) and without (n = 40) infectious disease. Allele frequency analysis identified 74 variants that were significantly (p < 5 × 10−6) associated with infectious disease, the majority of which were clustered in a 29-kb segment upstream of the collectin locus on chromosome 28. In silico analysis of the functional effects of all the variants predicted 11 SNVs with a deleterious effect on protein structure and/or function, 148 SNVs that occurred within potential transcription factor binding sites, and 31 SNVs occurring within potential miRNA binding elements. This study provides a detailed look at the genetic variation of the bovine collagenous lectins and identifies potential genetic markers for infectious disease susceptibility.Electronic supplementary materialThe online version of this article (10.1007/s00251-018-1061-7) contains supplementary material, which is available to authorized users.

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Chicken mannose-binding lectin (MBL) gene variants with influence on MBL serum concentrations

Cited by 16 publications

References 52 publications

Characterization of Cellular and Humoral Immune Responses After IBV Infection in Chicken Lines Differing in MBL Serum Concentration

Characterization of Cellular and Humoral Immune Responses After IBV Infection in Chicken Lines Differing in MBL Serum Concentration

Adjuvant effects of mannose-binding lectin ligands on the immune response to infectious bronchitis vaccine in chickens with high or low serum mannose-binding lectin concentrations

Identification of polymorphisms in the bovine collagenous lectins and their association with infectious diseases in cattle

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