Chiral Method by Normal Phase HPLC–UV for Quantitation of Lumefantrine Enantiomers in Tablet Formulations

We developed and validated a simple, sensitive, selective, and reliable LC–MS/MS–ESI method for the direct quantitation of lumefantrine (LFN) enantiomers [(−)‐LFN and (+)‐LFN] in mice plasma as per regulatory guideline. LFN enantiomers and carbamazepine (internal standard) were extracted from mice plasma using Strata X SPE (solid‐phase extraction) cartridges. Good resolution between enantiomers was achieved on a Chiralpak IA‐3 column using an isocratic mobile phase (0.1% of diethyl amine in methanol), which was delivered at a flow rate of 0.8 mL/min. Detection and quantitation were performed using multiple reaction monitoring mode following the transitions m/z 530.27 → 512.30 and 237.00 → 194.00 for LFN enantiomers and the internal standard, respectively, in the positive‐ionization mode. The proposed method provided accurate and reproducible results over the linearity range of 2.39–895 ng/mL for each enantiomer. The intra‐ and inter‐day precisions were in the range of 1.03–6.14 and 6.36–8.70 and 2.03–4.88 and 5.82–11.5 for (−)‐LFN and (+)‐LFN, respectively. Both (−)‐LFN and (+)‐LFN were found to be stable under different stability conditions. The method was successfully used to delineate stereoselective pharmacokinetics of LFN enantiomers in mice after an oral administration of rac‐LFN (20 mg/kg). The pharmacokinetic results indicated that the disposition of LFN enantiomers was stereoselective in mice.

show abstract

Enantioselective LC–ESI–MS/MS method for quantitation of (−)‐lumefantrine and (+)‐lumefantrine in mice plasma and application to a pharmacokinetic study

Kiran

Balaji

Gabani

et al. 2020

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Chiral Method by Normal Phase HPLC–UV for Quantitation of Lumefantrine Enantiomers in Tablet Formulations

Cited by 1 publication

References 31 publications

Enantioselective LC–ESI–MS/MS method for quantitation of (−)‐lumefantrine and (+)‐lumefantrine in mice plasma and application to a pharmacokinetic study

Enantioselective LC–ESI–MS/MS method for quantitation of (−)‐lumefantrine and (+)‐lumefantrine in mice plasma and application to a pharmacokinetic study

Contact Info

Product

Resources

About