Chiral separation by capillary electrophoresis

Application of sulfobutylether-β-cyclodextrin with specific degrees of substitution for the enantioseparation of pharmaceutical mixtures by capillary electrophoresis

“…6, which plots mobility differences for (+)-and (±)-bidisomide versus selector concentration with selectors of various degrees of charge. The plots for HP-b-CD and SBE [1] ds (1) sr -b-CD approach characteristic optimum selector concentrations, as predicted by Eq. (1).…”

“…Many different types of additives have been successfully used as isomer selectors in capillary electrophoresis [1,2], including metal chelates [3], chiral surfactants [4±7], proteins [8,9], oligosaccharides [10,11], crown ethers [12±14], macrocyclic antibiotics [15,16], and cyclodextrins [1, 2, 17±33, 37, 44]. Differences in the stability of the selector-analyte complexes for each enantiomer result in chiral separation.…”

“…These materials were obtained from the Center for Drug Delivery Research (CDDR, Lawrence, KS) and were prepared and characterized as described previously [36,37]. The isolated pure charge types (SBE [1] ds (1) sr -b-CD, SBE [2] ds (1) sr -b-CD, SBE [3] ds (1) sr -b-CD, SBE [4] ds (1) sr -b-CD, and SBE [7] ds (1) sr -b-CD were also obtained from CDDR. These were isolated from the SBE-b-CD mixtures using preparative ion exchange chromatography, with the residual salts being removed by ultrafiltration [20].…”

Chiral Separations of Enantiomeric Pharmaceuticals by Capillary Electrophoresis Using Sulphobutyl Ether ?-Cyclodextrin as Isomer Selector

“…Many kinds of buffer additives have been successfully used as isomer selectors in CE, including metal complexes (Kuhn and Hoffstetter-Kuhn, 1992;Snopek et al, 1988), chiral surfactants (Gozel et al, 1987), proteins (Otsuka and Terabe, 1990), oligosaccharides (Barker et al, 1992), crown ethers (Dhulst & Verkeke, 1992), macrocyclic antibiotics (Otsuka et al, 1994), and cyclodextrins (Richard and Bopp, 1994;Richard et al, 1996;Armstrong et al, 1986Armstrong et al, , 1994Fanali, 1991;Smith, 1993;Schmitt and Engelhardt, 1993;Tait et al, 1994;Lunie et al, 1994;Chank Vetadze et al, 1994, 1995a. These selectors interact with analyte molecules to form complexes which have different mobilities than the free analyte, and therefore differences in the stability of the complexes between isomers result in stereoisomer resolution.…”

Teicoplanin-Based Enantiomeric Separations in CZE Using a Partial Filling Technique

“…Capillary zone electrophoresis (CZE) [4][5][6][7][8][9] has been applied to the separation of enantiomers, mainly dissolving the chiral selector in the background electrolyte (BGE). Differences in formation constants and/or in mobility of transient enantiomer-selector complexes are the main requirements for a successful CZE chiral separations.…”