Chitin Nanowhiskers Mediate Transformation of Escherichia coli by Exogenous Plasmid DNA

Mera, Alisa; Araki, Jun; Ohtsuki, Takashi; Shimosaka, Makoto; Yoshida, Naoto

doi:10.4172/2155-952x.1000114

Cited by 11 publications

(4 citation statements)

References 34 publications

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“…3). This efficiency is lower than the one obtained by Yoshida and colleagues using chitin nano-whiskers (2.6 × 10 5 CFU/pUC18) with JM109 cells on 2.5% agar hydrogels (Mera et al, 2011). Wilharm et al also using sepiolite obtained a higher efficiency for pUC19 and E coli TOP 10 cells (2 × 10 6 CFU/μg pUC19) (Wilharm et al, 2010).…”

Section: Discussionmentioning

confidence: 61%

Transformation of Escherichia coli JM109 using pUC19 by the Yoshida effect

Mendes

Vieira

Lanceros‐Méndez

et al. 2015

Journal of Microbiological Methods

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Transformation of non-competent Escherichia coli JM109 was accomplished using pUC19 as donor plasmid and sepiolite as the acicular material to promote cell piercing via application of friction with a polystyrene stick or a magnetic bar on the surface of a hydrogel containing agar. An automatic spreading setup was built with a conventional stirring plate and compared to manual spreading. Several parameters were optimized, namely, the agar content of the hydrogel (2%), concentration of cells (OD=1.3 corresponding to 1.4×10(9) bacterial cells/mL), concentration of sepiolite (0.01%), manual versus mechanical spreading (automatic spreading more consistent) and spreading time (30s). Efficiency values up to 4.1×10(4) CFU/μg pUC19 were obtained. The method proved to be suitable for a rapid and low cost transformation of non-competent E. coli JM109, where higher values of efficiency do not need to be attained.

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Section: Discussionmentioning

confidence: 61%

Transformation of Escherichia coli JM109 using pUC19 by the Yoshida effect

Mendes

Vieira

Lanceros‐Méndez

et al. 2015

Journal of Microbiological Methods

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“…The acid hydrolysis produced during the extraction of chitin nanocrystals enlarges the proportion of deacetylated groups with the outcome of high antibacterial activity [ 54 , 55 ]. The hydrolytic treatment leads to the formation of NH 3 + groups, which interact with the negatively charged residues of carbohydrates, lipids, and proteins located on the cell surface of bacteria, so explaining their role in bacterial impairment [ 56 , 57 ]. Previous studies support the model in which the electrostatic forces between protonated –NH 3 + groups and the negative residues mediated the interaction, presumably by competing with Ca 2 + for electronegative sites on the membrane surface [ 7 , 49 ].…”

Section: Resultsmentioning

confidence: 99%

Water Filtration Membranes Based on Non-Woven Cellulose Fabrics: Effect of Nanopolysaccharide Coatings on Selective Particle Rejection, Antifouling, and Antibacterial Properties

et al. 2021

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This article presents a comparative study of the surface characteristics and water purification performance of commercially available cellulose nonwoven fabrics modified, via cast coating, with different nano-dimensioned bio-based carbohydrate polymers, viz. cellulose nanocrystals (CNC), TEMPO-oxidized cellulose nanofibers (T-CNF), and chitin nanocrystals (ChNC). The surface-modified nonwoven fabrics showed an improvement in wettability, surface charge modification, and a slight decrease of maximum pore size. The modification improved the water permeance in most of the cases, enhanced the particle separation performance in a wide range of sizes, upgraded the mechanical properties in dry conditions, and showed abiotic antifouling capability against proteins. In addition, T-CNF and ChNC coatings proved to be harmful to the bacteria colonizing on the membranes. This simple surface impregnation approach based on green nanotechnology resulted in highly efficient and fully bio-based high-flux water filtration membranes based on commercially available nonwoven fabrics, with distinct performance for particle rejection, antifouling and antibacterial properties.

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“…Apart from these there were some natural polymers that include Starch, chitosan [33], alginate, and dextrans. These polymers are applied as colloidal particles of size 10nm-1μm termed nano-particles.…”

Section: Engineering Of Nanotubesmentioning

confidence: 99%

Nanomedical Platform for Drug Delivery

P¹

2011

J Nanomed Nanotechnol

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Nanoparticles hold tremendous potential as an effective drug delivery system. The developments in nanotechnology for drug delivery laid platform for nanomedicine. To achieve efficient drug delivery it is important to understand the interactions of nanomaterials with the biological environment. This rapidly growing field allows cross-disciplinary researchers the opportunity to design and develop multifunctional nanoparticles in the form of nanotubes that can target, diagnose, and treat diseases such as cancer. Here I discussed about the engineering of nanotubes which targets drug delivery, barriers of drug delivery and advanced drug delivery systems.

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Chitin Nanowhiskers Mediate Transformation of Escherichia coli by Exogenous Plasmid DNA

Cited by 11 publications

References 34 publications

Transformation of Escherichia coli JM109 using pUC19 by the Yoshida effect

Transformation of Escherichia coli JM109 using pUC19 by the Yoshida effect

Water Filtration Membranes Based on Non-Woven Cellulose Fabrics: Effect of Nanopolysaccharide Coatings on Selective Particle Rejection, Antifouling, and Antibacterial Properties

Nanomedical Platform for Drug Delivery

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