Chitinophaga alhagiae sp. nov., isolated from rhizosphere soil of Alhagi sparsifolia

Zou, Yuanyuan; Zhang, Xiaoxia; Song, Haozhi; Liu, Yang; Cheng, Qi

doi:10.1099/ijsem.0.003292

Cited by 11 publications

(9 citation statements)

References 29 publications

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“…Correspondingly, the two genome sequences showed N50 values of 511 191 and 656 932 bp with G+C contents of 45. 6 [26] and 70 % dDDH [25] suggested for species discrimination, implying that strain ysch24 T should represent a distinct genotypic species.…”

Section: Genome Featuresmentioning

confidence: 99%

“…dsmz. de/ genus/ chitinophaga) including the recently described Chitinophaga lutea [3], Chitinophaga deserti [4], Chitinophaga silvisoli [5], Chitinophaga alhagiae [6] and Chitinophaga flava [7]. Strains of the genus Chitinophaga are characterized by Gram-stain-negative, filamentous or rod-shaped cells, hydrolysing chitin or cellobiose and having iso-C 15 : 0 , C 16 : 1 ω5c and iso-C 17 : 0 3-OH as major fatty acids and menaquinone 7 (MK-7) as the major respiratory quinone [2].…”

mentioning

confidence: 99%

“…Strains of the genus Chitinophaga are characterized by Gram-stain-negative, filamentous or rod-shaped cells, hydrolysing chitin or cellobiose and having iso-C 15 : 0 , C 16 : 1 ω5c and iso-C 17 : 0 3-OH as major fatty acids and menaquinone 7 (MK-7) as the major respiratory quinone [2]. They have been isolated from various niches such as contaminated, desert or forest soils, plant root, sediment, activated sludge, etc [3][4][5][6][7][8][9][10].…”

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confidence: 99%

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Chitinophaga tropicalis sp. nov., isolated from forest soil

Zhang

Feng

Yao

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A novel bacterial strain, designated ysch24T, was isolated from a forest soil sample collected from the Cat Tien National Park, southern Vietnam. Cells were Gram-stain-negative, aerobic, gliding, filamentous or rod-shaped. The results of 16S rRNA gene analyses revealed that strain ysch24T belongs to the genus Chitinophaga , and was most closely related to Chitinophaga silvisoli GDMCC 1.1411T (97.4 %), followed by Chitinophaga oryziterrae JCM 16595T (97.3 %) and Chitinophaga sancti NBRC 15057T (96.9 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain ysch24T and closely related type strains were 72.0–74.0 % and 19.1–19.4 %, respectively. Major fatty acids were iso-C15 : 0, C16 : 1 ω5c and iso-C17 : 0 3-OH and the predominant respiratory quinone was menaquinone 7. Polar lipids consisted of phosphatidylethanolamine, four unidentified aminophospholipids, two unidentified phospholipids and four unidentified lipids. The genomic DNA G+C content was 45.6 mol%. The study clearly showed that strain ysch24T should represent a novel species of the genus Chitinophaga , for which the name Chitinophaga tropicalis sp. nov. is proposed. The type strain is ysch24T (=GDMCC 1.1355T=KACC 21527T).

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Section: Genome Featuresmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Chitinophaga tropicalis sp. nov., isolated from forest soil

Zhang

Feng

Yao

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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“…The genus Chitinophaga was first described in the 1980s and is characterized as a gram-negative bacteria, positive oxidase and non-pathogenic and mesophilic characteristics [16], with a filamentous morphology that transforms when aging into spherical bodies (mixospores), without the production of a fruiting body [17]. The genus stands out for participating actively in soils [18][19][20], specifically in the rhizosphere [21], arsenic-contaminated soil [22] and vermicompost [23], where the biomass of plants and fungi is intensely degraded. This degradation is extremely significant for carbon recycling in the natural environment [24].…”

Section: Introductionmentioning

confidence: 99%

From Data Mining of Chitinophaga sp. Genome to Enzyme Discovery of a Hyperthermophilic Metallocarboxypeptidase

et al. 2021

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For several centuries, microorganisms and enzymes have been used for many different applications. Although many enzymes with industrial applications have already been reported, different screening technologies, methods and approaches are constantly being developed in order to allow the identification of enzymes with even more interesting applications. In our work, we have performed data mining on the Chitinophaga sp. genome, a gram-negative bacterium isolated from a bacterial consortium of sugarcane bagasse isolated from an ethanol plant. The analysis of 8 Mb allowed the identification of the chtcp gene, previously annotated as putative Cht4039. The corresponding codified enzyme, denominated as ChtCP, showed the HEXXH conserved motif of family M32 from thermostable carboxypeptidases. After expression in E. coli, the recombinant enzyme was characterized biochemically. ChtCP showed the highest activity versus benziloxicarbonil Ala-Trp at pH 7.5, suggesting a preference for hydrophobic substrates. Surprisingly, the highest activity of ChtCP observed was between 55 °C and 75 °C, and 62% activity was still displayed at 100 °C. We observed that Ca2+, Ba2+, Mn2+ and Mg2+ ions had a positive effect on the activity of ChtCP, and an increase of 30 °C in the melting temperature was observed in the presence of Co2+. These features together with the structure of ChtCP at 1.2 Å highlight the relevance of ChtCP for further biotechnological applications.

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“…Members of the genus were isolated from various sources, e.g. soil [2][3][4], activated sludge [5], plants [6][7][8] and humans [9]. Cells of Chitinophaga species were rod-shaped and Gramstain-negative [2].…”

Section: Introductionmentioning

confidence: 99%

Chitinophaga oryzae sp. nov., an epiphytic bacterium isolated from rice root surfaces

Niemhom

Suriyachadkun

Kittiwongwattana

2021

International Journal of Systematic and Evolutionary Microbiology

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Two Gram-stain-negative, non-motile, rod-shaped bacterial strains were isolated from the surfaces of rice roots. They were designated as strains 1303T and 1310. Their colonies were circular, entire, opaque, convex and yellow. They were chitinase- and catalase-positive, reduced nitrate and grew at 16–37 °C (optimum, 30 °C), pH 5.0–10.0 (optimum, pH 7.0) and 0–2.0% NaCl (optimum, 1.0 %). Based on the 16S rRNA gene sequence analysis, they were classified as members of the genus Chitinophaga . Results of phylogenetic and phylogenomic analyses indicated that they formed a cluster with Chitinophaga eiseniae YC6729T, Chitinophaga qingshengii JN246T, Chitinophaga varians 10-7 W-9003T and Chitinophaga fulva G-6-1-13T. When the genomic sequences of strains 1303T and 1310 were compared with their close relatives, the average nucleotide identity and digital DNA–DNA hybridization values were below the cut-off levels. Phosphatidylethanolamine was the major polar lipid. MK-7 was the major respiratory quinone. iso-C15 : 0, C16 : 1 ω5c, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c) were the predominant fatty acids. Differential characteristics between both strains and their close relatives were also observed. Based on the distinctions in genotypic, phenotypic and chemotypic features, strains 1303T and 1310 represent members of a novel species of the genus Chitinophaga , for which the name Chitinophaga oryzae sp. nov. is proposed. The type strain is 1303T (=KACC 22075T=TBRC 12926T).

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Chitinophaga alhagiae sp. nov., isolated from rhizosphere soil of Alhagi sparsifolia

Cited by 11 publications

References 29 publications

Chitinophaga tropicalis sp. nov., isolated from forest soil

Chitinophaga tropicalis sp. nov., isolated from forest soil

From Data Mining of Chitinophaga sp. Genome to Enzyme Discovery of a Hyperthermophilic Metallocarboxypeptidase

Chitinophaga oryzae sp. nov., an epiphytic bacterium isolated from rice root surfaces

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