Chitosan-based enzyme ink for screen-printed bioanodes

Shitanda, Isao; Oda, Kanako; Loew, Noya; Watanabe, Hikari; Itagaki, Masayuki; Tsujimura, Seiya; Zebda, Abdelkader

doi:10.1039/d1ra03277a

Cited by 6 publications

(3 citation statements)

References 49 publications

(70 reference statements)

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“…It is known that the physical properties of the polymer incorporated in enzyme ink are particularly important in determining enzyme transfer efficiency during μCP. 25 The key to enable high enzyme loading without surface spreading on a hydrophilic surface is to add a hydrophilic polymer with sufficiently strong intermolecular interactions, due to for example, entanglements, hydrogen bonding and van der Waals forces. Initially, experiments were conducted with poly( N -(3-aminopropyl)methacrylamide) (PAPM), a polymer of easily tuned length through free-radical polymerization that also provides free amine-groups for GAH crosslinking (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Microcontact printing of choline oxidase using a polycation-functionalized zwitterionic polymer as enzyme immobilization matrix

Zhao,

Cao,

Huang

et al. 2023

Analyst

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Section: Resultsmentioning

confidence: 99%

Microcontact printing of choline oxidase using a polycation-functionalized zwitterionic polymer as enzyme immobilization matrix

Zhao,

Cao,

Huang

et al. 2023

Analyst

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“…3D). A more detailed comparison of major features between different glucose sensors has been provided in table S4 (25,(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48), and the bioresorbable glucose sensor has a detection limit of 0.1 mM, which is compared much better than many conventional glucose sensors whose WEs do not contain nanostructures. Considering the typical fasting blood glucose concentration is 3.9 mM, the detection limit of the bioresorbable glucose sensor should be sufficient to meet daily and clinical needs.…”

Section: In Vitro Measurement Of the Bioresorbable Electrochemical De...mentioning

confidence: 99%

Fully printed and self-compensated bioresorbable electrochemical devices based on galvanic coupling for continuous glucose monitoring

Liu

et al. 2023

Sci. Adv.

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Real-time glucose monitoring conventionally involves non-bioresorbable semi-implantable glucose sensors, causing infection and pain during removal. Despite bioresorbable electronics serves as excellent alternatives, the bioresorbable sensor dissolves in aqueous environments with interferential biomolecules. Here, the theories to achieve stable electrode potential and accurate electrochemical detection using bioresorbable materials have been proposed, resulting in a fully printed bioresorbable electrochemical device. The adverse effect caused by material degradation has been overcome by a molybdenum-tungsten reference electrode that offers stable potential through galvanic-coupling and self-compensation modules. In vitro and in vivo glucose monitoring has been conducted for 7 and 5 days, respectively, followed by full degradation within 2 months. The device offers a glucose detection range of 0 to 25 millimolars and a sensitivity of 0.2458 microamperes per millimolar with anti-interference capability and biocompatibility, indicating the possibility of mass manufacturing high-performance bioresorbable electrochemical devices using printing and low-temperature water-sintering techniques. The mechanisms may be implemented developing more comprehensive bioresorbable sensors for chronic diseases.

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“…In 1987, genipin was observed to undergo a quick reaction with the simplest amino acid, glycine [6] . Later on, genipin has been commonly used to react with other amino acids, [7] and more complex amine‐containing structures such as chitosan, [8,9] collagen, [10] gelatin, [11] and enzymes [12] . Similar to other bifunctional crosslinkers, genipin can act as a “staple” of amine‐containing polymers to finally give stable crosslinked matrixes (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Study of Genipin Behavior in Neutral and Acidic Aqueous Solutions at Elevated Temperatures

2023

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Genipin is known to polymerize under basic conditions, and the obtained genipin oligomers might have an impact on the chemical and physical properties of the crosslinked materials. In this study, we intend to mimic the experimental condition in which genipin is used as a chemical crosslinker. We focus on the degradation of genipin at neutral and slightly acidic conditions using NMR. We report genipin polymerization at neutral and acidic conditions. Unlike dialdehyde crosslinkers, genipin requires heating to promote its crosslinking activity. We found that elevated temperatures enhance the degradation of genipin and promote its polymerization.

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Chitosan-based enzyme ink for screen-printed bioanodes

Abstract: Bio-composite inks based on magnesium oxide (MgO)-templated mesoporous carbon (MgOC) and chitosan cross-linked with genipin for one-step screen-printing process.

Cited by 6 publications

References 49 publications

Microcontact printing of choline oxidase using a polycation-functionalized zwitterionic polymer as enzyme immobilization matrix

Microcontact printing of choline oxidase using a polycation-functionalized zwitterionic polymer as enzyme immobilization matrix

Fully printed and self-compensated bioresorbable electrochemical devices based on galvanic coupling for continuous glucose monitoring

Study of Genipin Behavior in Neutral and Acidic Aqueous Solutions at Elevated Temperatures

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