Chitosan nanoparticles enhance the efficiency of methylene blue-mediated antimicrobial photodynamic inactivation of bacterial biofilms: An in vitro study

Darabpour, Esmaeil; Kashef, Nasim; Mashayekhan, Shohreh

doi:10.1016/j.pdpdt.2016.04.009

Cited by 81 publications

(52 citation statements)

References 34 publications

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“…The use of nanotechnology is an attractive approach to improving the effectiveness of APDI against resistant microbial cells [128–133] and to improve PS delivery to biofilms [134–136]. This goal may be achieved in different ways, for instance by enhancing the delivery of PS to microorganisms (encapsulating the PS in nanoparticles) [137] or by increasing the 1 O 2 yield of the PS (by covalently binding the PS to the surface of the nanoparticles [138], or simply by mixing the nanoparticles and PS together).…”

Section: Biofilm Resistance To Apdimentioning

confidence: 99%

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Kashef

Hamblin

2017

Drug Resistance Updates

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255

173

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Infections have been a major cause of disease throughout the history of humans on earth. With the introduction of antibiotics, it was thought that infections had been conquered. However, bacteria have been able to develop resistance to antibiotics at an exponentially increasing rate. The growing threat from multi-drug resistant organisms calls for intensive action to prevent the emergence of totally resistant and untreatable infections. Novel, non-invasive, non-antibiotic strategies are needed that act more efficiently and faster than current antibiotics. One promising alternative is antimicrobial photodynamic inactivation (APDI), an approach that produces reactive oxygen species when dyes and light are combined. So far, it has been questionable if bacteria can develop resistance against APDI. This review paper gives an overview of recent studies concerning the susceptibility of bacteria towards oxidative stress, and suggests possible mechanisms of the development of APDI-resistance that should at least be addressed. Some ways to potentiate APDI and also to overcome future resistance are suggested.

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Section: Biofilm Resistance To Apdimentioning

confidence: 99%

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Kashef

Hamblin

2017

Drug Resistance Updates

Self Cite

255

173

View full text Add to dashboard Cite

show abstract

“…Oxygen species that are capable of eliminating target cells by oxidative stress to cell membranes and other cellular parts. 9 Photosensitizers An optimally photosensitizers ought to have favorable structural features including physical, chemical, and biological. Many optical photosensitizers for photodynamic therapy have been tested in vitro as well as in vivo conditions.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Photodynamic Therapy: An Effective Alternative Approach to Control Bacterial Infections

et al. 2018

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Introduction: The purpose of this review was to evaluate the available literature for in vitro and in vivo effectiveness of antimicrobial Photodynamic therapy (aPDT) in the field of bacteriology. Methods: A review of the relevant articles carried out in PubMed and Scopus to determine the efficiency of aPDT used in the reduction of microbial infection. Thirty-one relevant documents retrieved from PubMed, Scopus by inserting "antimicrobial photodynamic therapy" and "bacterial infection" and "photodynamic therapy" keywords. Results: According to different results, aPDT can be used as an adjuvant for the treatment of infectious diseases. The use of photosensitizer methylene blue, toluidine blue O (TBO), indocyanine green with light diode laser centered at (630±10 nm) and (650±10 nm) wavelengths have been shown to have significant results for the treatment of infectious diseases and bactericidal properties Conclusion: These findings suggest that, aPDT can be an efficient method in the treatment of localized and superficial infections.

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“…MB-based PDT eradicated P. aeruginosa even after biofilm formation; however, the disinfection effect of PDT in the biofilm state was less than that in the planktonic state. The reduced antibacterial effect of PDT has been demonstrated in previous studies (10,13,14,16), and the ability of bacteria to form biofilms is an important factor that can lead to endoscopy associated infections. Many bacteria, including P. aeruginosa and atypical mycobacteria, can exist in a planktonic state and produce biofilms (17).…”

Section: Discussionmentioning

confidence: 85%

Hydrogen Peroxide Enhances the Antibacterial Effect of Methylene Blue‐based Photodynamic Therapy on Biofilm‐forming Bacteria

Yang

Lee

Park

et al. 2018

Photochem & Photobiology

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Recently, increased attention has been focused on endoscopic disinfection after outbreaks of drug‐resistant infections associated with gastrointestinal endoscopy. The aims of this study were to investigate the bactericidal efficacy of methylene blue (MB)‐based photodynamic therapy (PDT) on Pseudomonas aeruginosa (P. aeruginosa), which is the major cause of drug‐resistant postendoscopy outbreak, and to assess the synergistic effects of hydrogen peroxide addition to MB‐based PDT on biofilms. In planktonic state of P. aeruginosa, the maximum decrease was 3 log10 and 5.5 log10 at 20 and 30 J cm−2, respectively, following MB‐based PDT. However, the maximum reduction of colony forming unit (CFU) was decreased by 2.5 log10 and 3 log10 irradiation on biofilms. The biofilm formation was significantly inhibited upon irradiation with MB‐based PDT. When the biofilm state of P. aeruginosa was treated with MB‐based PDT with hydrogen peroxide, the CFU was significantly decreased by 6 log10 after 20 J cm−2, by 7 log10 after 30 J cm−2 irradiation, suggesting significantly higher efficacy than MB‐based PDT alone. The implementation of the combination of hydrogen peroxide with MB‐based PDT through working channels might be appropriate for preventing early colonization and biofilm formation in the endoscope and postendoscopy outbreak.

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Chitosan nanoparticles enhance the efficiency of methylene blue-mediated antimicrobial photodynamic inactivation of bacterial biofilms: An in vitro study

Cited by 81 publications

References 34 publications

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Can microbial cells develop resistance to oxidative stress in antimicrobial photodynamic inactivation?

Antimicrobial Photodynamic Therapy: An Effective Alternative Approach to Control Bacterial Infections

Hydrogen Peroxide Enhances the Antibacterial Effect of Methylene Blue‐based Photodynamic Therapy on Biofilm‐forming Bacteria

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