Chitosan/o-carboxymethyl chitosan nanoparticles for efficient and safe oral anticancer drug delivery: In vitro and in vivo evaluation

Surface charge effect on mucoadhesion of chitosan based nanogels for local anti-colorectal cancer drug delivery

“…But for CS/CMCS/TPP NGs, CS-TPP core formed firstly and was then coated by CMCS giving negative surface potential. The absolute value of surface potential for both formulations with or without DOX in D-Hanks and DMEM were slightly lower than that in H 2 O, but still higher than 30 mV, which could maintain a favorable colloidal stability due to sufficient electrostatic repulsion [19,32].…”

“…CS/CMCS/TPP NGs were prepared according to a modified process originally based on our previous work [19,20]. Briefly, 2 mL of TPP (0.25 mg/mL) was added into 3 mL of CS solution (1 mg/mL) under magnetic stirring for 30 min.…”

“…CMCS was covalently labeled by FITC through amide bond formation between primary amino group on CMCS and isothiocyanate group on FITC at pH 6.9, and then dialyzed in tri-distilled water for 3 days to remove unreacted FITC refer to previous report [19,20]. FITC labled DOX free and DOX loaded NGs were prepared as described in Section 2.2.…”

“…Chitosan (CS), a nature polycation derived from chitin, has strong mucoadhesive properties and is able to promote cellular uptake into cancer cells and improve anticancer effects [18]. We previously reported the formulation and characterization of mucoadhesive doxorubicin hydrochloride (DOX) loaded nanogels (DOX:CS/CMCS NGs) composed of chitosan and carboxymethyl chitosan (CMCS) [19]. The drug release rate of DOX:CS/CMCS NGs was much lower in simulating gastric acid environment(<18%) than that in simulating intestinal environment, which was in favor of targeted delivering drug to colon [20].…”

Chitosan: A Promising Substrate for Regenerative Medicine in Drug Formulation

“…These loaded nanoparticles showed high encapsulation and loading efficacy efficiency of 73 and 9 % facilitated the transport of cyclosporin A to the inner eye, and significantly increased the bioavailability [54]. The oral bioavailability of paclitaxel loaded in N-octyl-O-sulfate chitosan micelles was improved sixfold in comparison with that Oral delivery system, prolong retention of drug in liver, spleen, lung and decrease level of drug in heart and kidney [67] (continued) of free paclitaxel, which was resulted from the interference of P-gp ATPase rather than down-regulation of P-gp expression by N-octyl-O-sulfate chitosan [55]. Jain et al [56] revealed that glutamate chitosan can enhance the transport of insulin across the nasal mucosa of sheep and rats.…”