The feed additives are the essential compound of the fish diet. The use of feed additives in the animal's diet is a vital role to rising growth and immunity of the aquatic animals. Feed additives are substances which are added in trace amounts in fish diet it is also act as ingredients improver or preserve it. The major feed additives are act as preservatives, binders, feeding stimulants, and food colorants etc. In favor of growing aquaculture in a supportable fashion, the use of additives must be in correct amount. The requirement of additives are depends on species and their availability. Some authors exposed that the feed additive to have antimicrobial, anti-oxidative, growth promoting ability as well as it is improve the fish immune system.

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Chitosan plays a most important role in the regenerative medication for wound healing. The adhesive nature of chitosan, with their antifungal and bactericidal character, and their permeability to oxygen, is a very important property associated with the treatment of wounds. Different derivatives and combination of chitosan have been reported for this purpose in the form of hydrogels, fibers, membranes, scaffolds and sponges. The purpose of the chapter is to have a closer look in the work done directly by different researchers on the chitosan formulation with potential medicinal applications to provide a better understanding of its usability in regenerative medicine.

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