Chitosan–Platelet Interactions

Hoemann, Caroline D.; Rivard, Georges‐Étienne

doi:10.1007/12_2021_86

Cited by 4 publications

(4 citation statements)

References 76 publications

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“…This interaction induces the adhesion, activation, and aggregation of blood cells and platelets to accelerate hemostasis, which is not related to the blood‐clotting pathway of the host. [ 17a,41 ] The hydrophobic chain of QCSL can enhance hemostatic activity via strong interactions between the hydrophobic chain and blood cell membranes. [ 22 ] Moreover, the pyrogallol groups can enhance the affinity of the hydrogel for blood cells and platelets, [ 42 ] leading to an enhanced procoagulant capacity of the hydrogel precursor or hydrogel interface.…”

Section: Resultsmentioning

confidence: 99%

Injectable Self‐Expanding/Self‐Propelling Hydrogel Adhesive with Procoagulant Activity and Rapid Gelation for Lethal Massive Hemorrhage Management

Zhao,

Huang,

et al. 2024

Advanced Materials

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Developing hydrogels that can quickly reach deep bleeding sites, adhere to wounds, and expand to stop lethal and/or noncompressible bleeding in civil and battlefield environments remains a challenge. Herein, we report an injectable, antibacterial, self‐expanding, and self‐propelling hydrogel bioadhesive with procoagulant activity and rapid gelation. This hydrogel combines spontaneous gas foaming and rapid Schiff base crosslinking for lethal massive hemorrhage. Hydrogels have rapid gelation and expansion rate, high self‐expanding ratio, excellent antibacterial activity, antioxidant efficiency, and tissue adhesion capacity. In addition, hydrogels have good cytocompatibility, procoagulant ability, and higher blood cell/platelet adhesion activity than commercial combat gauze and gelatin sponge. The optimized hydrogel (OD‐C/QGQL‐A30) exhibits better hemostatic ability than combat gauze and gelatin sponge in rat liver and femoral artery bleeding models, rabbit volumetric liver loss massive bleeding models with/without anticoagulant, and rabbit liver and kidney incision bleeding models with bleeding site not visible. Especially, OD‐C/QGQL‐A30 rapidly stops the bleedings from pelvic area of rabbit, and swine subclavian artery vein transection. Furthermore, OD‐C/QGQL‐A30 has biodegradability and biocompatibility, and accelerates MRSA‐infected skin wound healing. This injectable, antibacterial, self‐expanding and self‐propelling hydrogel opens up a new avenue to develop hemostats for lethal massive bleeding, abdominal organ bleeding, and bleeding from coagulation lesions.This article is protected by copyright. All rights reserved

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Section: Resultsmentioning

confidence: 99%

Injectable Self‐Expanding/Self‐Propelling Hydrogel Adhesive with Procoagulant Activity and Rapid Gelation for Lethal Massive Hemorrhage Management

Zhao,

Huang,

et al. 2024

Advanced Materials

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“…Compounds such as chitosan and other positively charged entities can readily form strong electrostatic interactions with platelets or other blood cells. [ 190 ] Incorporating chitosan into hydrogels can enrich platelets and promote coagulation. [ 191‐194 ] A carbon nanotube‐enhanced quaternary ammonium chitosan‐based cryogel has demonstrated remarkable hemostatic efficacy by exploiting the positive quaternary ammonium group and the amino group of quaternary ammonium chitosan to effectively adsorb platelets to the bleeding site.…”

Section: Design Of Bioactive Hydrogel For Hemostasismentioning

confidence: 99%

Bioactive Hydrogels with Pro‐coagulation Effect for Hemostasis^†

Guo,

Ding,

et al. 2023

Chin. J. Chem.

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Comprehensive SummaryHemostatic hydrogels are widely applied for wound management of damaged tissues, traumatic wounds, and surgical incisions. Some hydrogels composed of bioactive components, including fibrin and thrombin, showed great promise in the clinic due to their good pro‐coagulation effect. Based on the expanding knowledge of cascade catalysis reaction of coagulation and emerging bioactive substances. Recently, massive bioactive hydrogels based on peptides, hemocoagulase, polyphosphate (polyP), etc., have been developed as hemostatic materials. Based on the coagulation process and mechanism, we summarize the role of reported bioactive hydrogels in hemostasis in this review. We conclude the key points in the coagulation process, including activation of coagulation factors, fibrinogen polymerization, etc., then discuss how to design bioactive hydrogels to accelerate coagulation targeted to these points. Finally, we conclude the progress and propose a perspective of bioactive hydrogels with a pro‐coagulation effect for hemostasis.This article is protected by copyright. All rights reserved.

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“…Results from this study could improve our understanding of how endogenous HA levels are regulated in injured or arthritic joints. Data from this study could also provide valuable new ideas on how to utilize platelet-rich plasma (which releases PDGF and TGF-β1) to elicit chondroprotective responses in injured joints [ 12 – 14 ], and improve our understanding of how synoviocytes respond to inflammation.…”

Section: Introductionmentioning

confidence: 99%

UDP-glucose dehydrogenase (UGDH) activity is suppressed by peroxide and promoted by PDGF in fibroblast-like synoviocytes: Evidence of a redox control mechanism

et al. 2022

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UDP-glucose dehydrogenase (UGDH) generates essential precursors of hyaluronic acid (HA) synthesis, however mechanisms regulating its activity are unclear. We used enzyme histostaining and quantitative image analysis to test whether cytokines that stimulate HA synthesis upregulate UGDH activity. Fibroblast-like synoviocytes (FLS, from N = 6 human donors with knee pain) were cultured, freeze-thawed, and incubated for 1 hour with UDP-glucose, NAD+ and nitroblue tetrazolium (NBT) which allows UGDH to generate NADH, and NADH to reduce NBT to a blue stain. Compared to serum-free medium, FLS treated with PDGF showed 3-fold higher UGDH activity and 6-fold higher HA release, but IL-1beta/TGF-beta1 induced 27-fold higher HA release without enhancing UGDH activity. In selected proliferating cells, UGDH activity was lost in the cytosol, but preserved in the nucleus. Cell-free assays led us to discover that diaphorase, a cytosolic enzyme, or glutathione reductase, a nuclear enzyme, was necessary and sufficient for NADH to reduce NBT to a blue formazan dye in a 1-hour timeframe. Primary synovial fibroblasts and transformed A549 fibroblasts showed constitutive diaphorase/GR staining activity that varied according to supplied NADH levels, with relatively stronger UGDH and diaphorase activity in A549 cells. Unilateral knee injury in New Zealand White rabbits (N = 3) stimulated a coordinated increase in synovial membrane UGDH and diaphorase activity, but higher synovial fluid HA in only 2 out of 3 injured joints. UGDH activity (but not diaphorase) was abolished by N-ethyl maleimide, and inhibited by peroxide or UDP-xylose. Our results do not support the hypothesis that UGDH is a rate-liming enzyme for HA synthesis under catabolic inflammatory conditions that can oxidize and inactivate the UGDH active site cysteine. Our novel data suggest a model where UGDH activity is controlled by a redox switch, where intracellular peroxide inactivates, and high glutathione and diaphorase promote UGDH activity by maintaining the active site cysteine in a reduced state, and by recycling NAD+ from NADH.

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Chitosan–Platelet Interactions

Cited by 4 publications

References 76 publications

Injectable Self‐Expanding/Self‐Propelling Hydrogel Adhesive with Procoagulant Activity and Rapid Gelation for Lethal Massive Hemorrhage Management

Injectable Self‐Expanding/Self‐Propelling Hydrogel Adhesive with Procoagulant Activity and Rapid Gelation for Lethal Massive Hemorrhage Management

Bioactive Hydrogels with Pro‐coagulation Effect for Hemostasis^†

UDP-glucose dehydrogenase (UGDH) activity is suppressed by peroxide and promoted by PDGF in fibroblast-like synoviocytes: Evidence of a redox control mechanism

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Chitosan–Platelet Interactions

Cited by 4 publications

References 76 publications

Injectable Self‐Expanding/Self‐Propelling Hydrogel Adhesive with Procoagulant Activity and Rapid Gelation for Lethal Massive Hemorrhage Management

Injectable Self‐Expanding/Self‐Propelling Hydrogel Adhesive with Procoagulant Activity and Rapid Gelation for Lethal Massive Hemorrhage Management

Bioactive Hydrogels with Pro‐coagulation Effect for Hemostasis†

UDP-glucose dehydrogenase (UGDH) activity is suppressed by peroxide and promoted by PDGF in fibroblast-like synoviocytes: Evidence of a redox control mechanism

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Product

Resources

About

Bioactive Hydrogels with Pro‐coagulation Effect for Hemostasis^†