Chitosan–RGDSGGC conjugate as a scaffold material for musculoskeletal tissue engineering

Masuko, Tatsuya; Iwasaki, Norimasa; Yamane, Shintaro; Funakoshi, Tadanao; Majima, Tokifumi; Minami, Akio; Ohsuga, Noriko; Ohta, Takashi; Nishimura, Shin‐Ichiro

doi:10.1016/j.biomaterials.2005.01.062

Cited by 97 publications

(65 citation statements)

References 47 publications

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“…2-iminothiolane has been widely used to react with primary amines in CS 22, 23. The resulting thiolated CS showed a good solubility in water, which made it proper for the subsequent coupling reaction with peptide terminated with cysteine (containing thiol groups), as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Zero-order controlled release of BMP2-derived peptide P24 from the chitosan scaffold by chemical grafting modification technique for promotion of osteogenesis in vitro and enhancement of bone repair in vivo

Chen

Liu

et al. 2017

Theranostics

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Combination of tissue-engineered bone scaffolds with cell-adhesive, osteoconductive, or osteoinductive biomolecules is a critical strategy to improve their properties that significantly influence cellular behaviors, such as adhesion, proliferation, and differentiation, which is beneficial for critical-sized bone defects repairing. However, the traditional surface modification techniques, such as physical adsorption, coating, and plasma treatment, et al, have great limitations for immobilization of bioactive molecules due to undesirable controlled delivery performance or overly complex multistep procedures. In this study, we functionalized the chitosan/hydroxyapatite (CS/HA) biomimetic composite scaffold for controlled delivery of BMP2-derived peptide (P24) by the chemical grafting modification technique: firstly, P24 was conjugated with a thiolated chitosan, chitosan-4-thiobutylamidine (CS-TBA); secondly, the resultant CS-P24 was then combined with HA to prepare CS-P24/HA scaffolds. The effect of CS-P24/HA scaffolds on bone regeneration was evaluated, along with the underlying biological mechanisms responsible in vitro and in vivo. In vitro, the controlled and sustained release of bioactive P24 could last up to 90 days, furthermore, the release profiles of CS-5%P24/HA and CS-10%P24/HA were linear and could be fitted according to zero-order kinetic model (R2=0.9929; R2=0.9757); P24 on the scaffold significantly promoted cell adhesion, proliferation, osteodifferentiation, and mineralization with synergistic effects. Bone marrow stromal cells (BMSCs) revealed spindle-shaped surface morphology, indicating the CS-P24/HA scaffolds supported cell adhesion and possessed a high proliferation rate that varied according to the P24 concentration levels. Furthermore, mRNA levels for OCN, Runx2, and collagen I were significantly up-regulated on CS-P24/HA scaffolds compared with cells grown on CS/HA scaffolds in vitro (p < 0.05). Similarly, the BMSCs exhibited a higher ALP expression and calcium deposition level on CS-P24/HA scaffolds compared with CS/HA scaffolds (p < 0.05). In vivo, osteoinductive studies revealed a significantly higher ectopic osteogenesis level of CS-10%P24/HA scaffolds in rat dorsal muscle pockets compared with that of CS/HA scaffolds. Finally, CS-P24/HA scaffolds showed superior performance in the reconstruction of rat calvarial bone defects. This novel CS-P24/HA scaffold is deemed a strong potential candidate for the repair of bone defects in human bone tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Zero-order controlled release of BMP2-derived peptide P24 from the chitosan scaffold by chemical grafting modification technique for promotion of osteogenesis in vitro and enhancement of bone repair in vivo

Chen

Liu

et al. 2017

Theranostics

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“…Strong cell adhesion and proliferation in chondrocytes and fibroblasts (Masuko et al, 2005) N,O-CMC films Decreased blood clotting time and fibrin formation in human blood (Janvikul et al, 2006) …”

Section: Cmtmc and Sdgrg Complexesmentioning

confidence: 99%

Chitosan as a starting material for wound healing applications

Patrulea

Ostafe

Borchard

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

487

215

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Abstract:Chitosan and its derivatives have attracted great attention due to their properties beneficial for application to wound healing. The main focus of the present review is to summarize studies involving chitosan and its derivatives, especially N,N,N-trimethylchitosan (TMC), N,O-carboxymethyl-chitosan (CMC) and O-carboxymethyl-N,N,Ntrimethyl-chitosan (CMTMC), used to accelerate wound healing. Moreover, formulation strategies for chitosan and its derivatives, as well as their in vitro, in vivo and clinical applications in wound healing are described.

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“…Similarly, RGD-coated chitosan also demonstrated increased fibroblast proliferation (Masuko et al 2005).…”

Section: Functional Groupsmentioning

confidence: 96%

Scaffolds for tendon and ligament tissue engineering

Goh

Sahoo

2010

Regenerative Medicine and Biomaterials for the Repair of Connective Tissues

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Chitosan–RGDSGGC conjugate as a scaffold material for musculoskeletal tissue engineering

Cited by 97 publications

References 47 publications

Zero-order controlled release of BMP2-derived peptide P24 from the chitosan scaffold by chemical grafting modification technique for promotion of osteogenesis in vitro and enhancement of bone repair in vivo

Zero-order controlled release of BMP2-derived peptide P24 from the chitosan scaffold by chemical grafting modification technique for promotion of osteogenesis in vitro and enhancement of bone repair in vivo

Chitosan as a starting material for wound healing applications

Scaffolds for tendon and ligament tissue engineering

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