Chlorogenic acid protects against liver fibrosis in vivo and in vitro through inhibition of oxidative stress

Shi, Haitao; Shi, Ameng; Liu, Dong; Lu, Xiaolan; Wang, Yan; Zhao, Jiawei; Dai, Fei; Guo, Xiaoyan

doi:10.1016/j.clnu.2016.03.002

Cited by 157 publications

(122 citation statements)

References 46 publications

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“…Also, our study showed that treatment with the ROS inhibitor recovered the activation of TGF‐β/Smad signaling in cultured L02 cells, indicating that ROS production in response to AOPP treatment may provide a sustaining regulator and thereby stimulated TGF‐β/Smad signaling and trigger hepatocyte EMT. Besides the intracellular TGF‐β/Smad pathway, previous studies have confirmed that mitogen‐activated protein kinase (MAPK) and nuclear factor kappa B (NF‐κB)‐mediated signaling are also required for oxidative stress‐dependent liver fibrosis (Acquaviva et al, ; Cai et al, ; Shi et al, ). For these reasons, we plan to perform future studies on the roles of MAPK and NF‐κB signaling pathways in AOPP‐mediated hepatocyte EMT.…”

Section: Discussionmentioning

confidence: 99%

Advanced oxidation protein products induce hepatocyte epithelial–mesenchymal transition via a ROS‐dependent, TGF‐β/Smad signaling pathway

Sun

Xie

Zhang

et al. 2017

Cell Biology International

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Epithelial-mesenchymal transition (EMT) occurs during the progression of liver fibrosis in response to chronic liver injury. However, the molecular mechanism underlying the regulation of hepatocyte EMT remains unclear. The aim of this study was to determine whether advanced oxidation protein products (AOPP) had an effect on hepatocyte EMT. The human L02 hepatocyte cell line and hepatocytes from normal Sprague-Dawley rats were challenged with AOPP treatment in both in vitro and in vivo studies. The expression of cell and molecular markers of EMT in L02 hepatocytes were studied using Western blotting, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays. Hepatocyte migratory potential was analyzed using a wound healing assay. Intracellular reactive oxygen species (ROS) were detected using the dichlorofluorescein (DCF) assay. In liver tissue sections, expression of EMT markers was evaluated using immunohistochemistry, and collagen was assessed using histochemical staining with Masson's trichrome. The findings were that AOPP treatment resulted in EMT in hepatocytes, which was associated with reduced expression of E-cadherin, increased expression of vimentin, increased deposition of collagen protein, and enhanced cell migration in vivo and in vitro. AOPP was also found to promote migration in L02 cells, and to promote the production of ROS and the activation of TGF-βR and Smad signaling. Inhibition of the generation of intracellular ROS and TGF-β receptor blocking could reverse AOPP-induced EMT in hepatocytes. This study has identified a novel mechanism in the regulation of hepatocyte EMT, and the findings may have implications for the control of liver fibrosis.

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Section: Discussionmentioning

confidence: 99%

Advanced oxidation protein products induce hepatocyte epithelial–mesenchymal transition via a ROS‐dependent, TGF‐β/Smad signaling pathway

Sun

Xie

Zhang

et al. 2017

Cell Biology International

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“…This makes the native Huagalina potato a health-promoting agent as CGA acid and caffeic acid have been reported as inhibitors of carcinogenesis (Huang et al, 1998, Sancho and Pastore, 2012, Serafim et al, 2015, reducing obesity (Cho et al, 2010) hypotensive activity, providing cardiovascular protection (Susuki et al, 2002;Jiang et al, 2016). Shi et al (2016) showed that a new mechanism of CGA acid mediated in the protection of liver fibrosis (a stage before cirrhosis), inhibits oxidative stress and increases antioxidant defense and could become a promising new treatment for liver disease. Mikami and Yamazawa (2015) studied the protective effects of CGA acid on neuronal cell death induced by glutamate because the release of glutamate during cerebral ischemia triggers the death of neurons, suggesting that this polyphenol protects neurons from glutamate neurotoxicity by controlling calcium ion entry through glutamate receptors.…”

Section: Resultsmentioning

confidence: 99%

Phenolic compounds with antioxidant capacity of the native Andean potato (Solanum tuberosum L.) Huagalina variety in La Libertad- Peru

Rojas-Padilla

Vásquez-Villalobos

2016

Sc.Agropec

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Potato is grown and consumed throughout the world for their particular calorific nutritional value and vitamin C, but it also contains phytochemicals (secondary metabolites) that have been studied and found to have positive effects in preventing degenerative diseases in human health, such as hypertensive activity, and also atherosclerosis, type 2 diabetes, liver fibrosis, Alzheimer's disease, macular degeneration and cancer. We aimed to identify and quantify phenolic compounds in potatoes grown on El Zuro (EZ) and Huayatan Alto (HA) in Santiago de Chuco, La Libertad, Peru. The field trials were carried out in EZ (altitude 3750 m.a.s.l.) and HA (altitude 3150 m.a.s.l.) employing organic and inorganic fertilization respectively. Extraction from the peel and flesh was obtained separately, with the following solution: 50% methanol, 50% deionized water and 0.5% acetic acid. The sample was injected into the system UPLC -MS / MS, using ESI ionization (Electrospray Ionization) and fifteen external reference standards. Thirteen metabolites were detected in the flesh and potato peel. The highest content of secondary metabolites (mg/100 g DW) were: Chlorogenic acid (476.82 ± 63.58), neochlorogenic acid (87.90 ± 19.42) caffeic acid (77.53 ± 14.49) and vanillin (11.52 ± 1.38). The PCA (Principal Components Analysis) scores show that the highest concentration of metabolites was found in the peel of both cultivars. We concluded that the native potato Huagalina contains the genes expressed in different biosynthetic pathways of the metabolites found in this study.

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“…In addition, CGA could inhibit lipopolysaccharide (LPS)-induced microglial activation and improve the survival of dopaminergic neurons (21). CGA could also reduce carbon tetrachloride-induced liver fibrosis in rats through inhibition of the Toll-like receptor 4 signaling pathway (22,23) and through the suppression of oxidative stress in liver and hepatic stellate cells (24).…”

mentioning

confidence: 99%

Antischistosomiasis Liver Fibrosis Effects of Chlorogenic Acid through IL-13/miR-21/Smad7 Signaling Interactions In Vivo and In Vitro

Wang

Yang

Xue

et al. 2017

Antimicrob Agents Chemother

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Chlorogenic acid protects against liver fibrosis in vivo and in vitro through inhibition of oxidative stress

Cited by 157 publications

References 46 publications

Advanced oxidation protein products induce hepatocyte epithelial–mesenchymal transition via a ROS‐dependent, TGF‐β/Smad signaling pathway

Advanced oxidation protein products induce hepatocyte epithelial–mesenchymal transition via a ROS‐dependent, TGF‐β/Smad signaling pathway

Phenolic compounds with antioxidant capacity of the native Andean potato (Solanum tuberosum L.) Huagalina variety in La Libertad- Peru

Antischistosomiasis Liver Fibrosis Effects of Chlorogenic Acid through IL-13/miR-21/Smad7 Signaling Interactions In Vivo and In Vitro

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