Chlorophyll a, an active anti-proliferative compound of Ludwigia octovalvis, activates the CD95 (APO-1/CD95) system and AMPK pathway in 3T3-L1 cells

Wu, Shu-Jing; Ng, Lean‐Teik; Wang, Gueu-Horng; Huang, Shing Jong; Chen, Jinliang; Sun, Fang-Ming

doi:10.1016/j.fct.2009.12.001

Cited by 27 publications

(13 citation statements)

References 27 publications

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“…Various pharmacological activities of this plant have been reported e.g. antibacterial, antioxidant (Yakob et al, 2012a), antidiarrhoeal, antidiabetic, anti-inflammatory (Murugesan et al, 2002), anticancer (Wu et al, 2010) and hepatoprotective activities (Lin et al, 2006). However, the potential of this plant in the induction or enhancement of immune response against infectious diseases had not been evaluated.…”

Section: Introductionmentioning

confidence: 97%

Immune-stimulating properties of 80% methanolic extract of Ludwigia octovalvis against Shiga toxin-producing E. coli O157:H7 in Balb/c mice following experimental infection

Yakob

Uyub

Sulaiman

2015

Journal of Ethnopharmacology

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Section: Introductionmentioning

confidence: 97%

Immune-stimulating properties of 80% methanolic extract of Ludwigia octovalvis against Shiga toxin-producing E. coli O157:H7 in Balb/c mice following experimental infection

Yakob

Uyub

Sulaiman

2015

Journal of Ethnopharmacology

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“…In addition, SCC was shown to induce apoptosis in cancer cells through inhibition of the oncoprotein mitogen-activated protein kinase (MAPK) and signal transduction by the extracellular signal-regulated kinase (ERK) in breast cancer cells MCF-7 [ 11 ]. Wu et al [ 12 ] further illustrated that chlorophyll a in Ludwigia octovalvis could induce apoptosis of 3T3-L1 adipocytes through activation of the CD95 system for regulation of Bcl-2 family protein and activation of caspase-3, leading to the activation of AMP activated protein kinase (AMPK) signaling pathway for the inhibition of adipogenesis. In addition, both chlorophyll and chlorophyllin were shown to exhibit antimutagenic and anticancer properties in vivo and in vitro, probably caused by the binding of both to chemical mutagens or their metabolites for toxicity reduction [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Chlorophyll Nanoemulsion from Pomelo Leaves and Its Inhibition Effect on Melanoma Cells A375

Liu

Chen

2021

Plants

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Pomelo (Citrus grandis), an important fruit crop grown in tropical and subtropical areas, is cultivated mainly in Asian countries. The dominant pigment in pomelo leaves, chlorophyll, has been reported to possess many biological activities such as antioxidant, anti-inflammation and anticancer. The objectives of this study were to determine chlorophylls in Pomelo leaves by high-performance liquid chromatography-mass spectrometry (HPLC-MS) and to encapsulate the isolated chlorophylls from preparative column chromatography into a nanoemulsion system for elucidating the inhibition mechanism on the growth of melanoma cells A375. The results showed that chlorophyll a and chlorophyll b could be separated within 25 min by using a C18 column and a gradient ternary mobile phase of acetone, acetonitrile and methanol. Pomelo leaves mainly contained chlorophyll a (2278.3 μg/g) and chlorophyll b (785.8 μg/g). A highly stable chlorophyll nanoemulsion was prepared with the mean particle size being 13.2 nm as determined by a dynamic light scattering (DLS) method. The encapsulation efficiency of chlorophyll nanoemulsion was 99%, while the zeta potential was −64.4 mV. In addition, the chlorophyll nanoemulsion possessed high thermal stability up to 100 °C and remained stable over a 90-day storage period at 4 °C. Western blot analysis revealed that chlorophyll nanoemulsion and extract could upregulate p53, p21, cyclin B and cyclin A as well as downregulate CDK1 and CDK2 in a concentration-dependent manner for inhibition of melanoma cells A375. Furthermore, chlorophyll nanoemulsion and extract could upregulate Bax and cytochrome C and downregulate Bcl-2, leading to activation of caspase-9, caspase-8 and caspase-3 for the induction of cell apoptosis. Compared to chlorophyll extract, chlorophyll nanoemulsion was more effective in inhibiting the growth of melanoma cells A375.

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“…Previous phytochemical studies have described the presence of flavonoids, phenolic acids, polyphenols, saponins, sterols, tannins, and triterpenoids [ 12 – 15 ] in different organs of this medicinal plant. Several pharmacological effects such as hypoglycaemic [ 8 ], antihyperglycaemic [ 16 , 17 ], and antiproliferative, in 3T3–L1 adipocytes [ 18 ], have been described through various models. Moreover, the hydroalcoholic extract of L. octovalvis leaves was the most effective in the inhibition of α –glucosidases and pancreatic lipase in a screening of 23 extracts of medicinal plants reported as traditional treatments for type 2 diabetes mellitus [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Digestive Enzyme Inhibitors from Ludwigia octovalvis (Jacq.) P.H.Raven

Ramírez

Herrera-Arellano

Tortoriello

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Current antiobesity and antidiabetic tools have been insufficient to curb these diseases and frequently cause side effects; therefore, new pancreatic lipase and α–glucosidase inhibitors could be excellent aids for the prevention and treatment of these diseases. The aim of this study was to identify, quantify, and characterize the chemical compounds with the highest degree of inhibitory activity of these enzymes, contained in a Ludwigia octovalvis hydroalcoholic extract. Chemical purification was performed by liquid–liquid separation and column chromatography. Inhibitory activities were measured in vitro, employing acarbose, orlistat, and a Camellia sinensis hydroalcoholic extract as references. For structural elucidation, Nuclear Magnetic Resonance was carried out, and High Performance Liquid Chromatography was used to quantify the compounds. For α–glucosidases, L. octovalvis hydroalcoholic extract and its ethyl acetate fraction showed half–maximal Inhibitory Concentration (IC50) values of 700 and 250 μg/mL, for lipase, 480 and 718 μg/mL, while C. sinensis showed 260 and 587 μg/mL. The most active compounds were identified as ethyl gallate (1, IC50 832 μM) and gallic acid (2, IC50 969 μM); both displayed competitive inhibition of α–glucosidases and isoorientin (3, IC50 201 μM), which displayed uncompetitive inhibition of lipase. These data could be useful in the development of a novel phytopharmaceutical drug.

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Chlorophyll a, an active anti-proliferative compound of Ludwigia octovalvis, activates the CD95 (APO-1/CD95) system and AMPK pathway in 3T3-L1 cells

Cited by 27 publications

References 27 publications

Immune-stimulating properties of 80% methanolic extract of Ludwigia octovalvis against Shiga toxin-producing E. coli O157:H7 in Balb/c mice following experimental infection

Immune-stimulating properties of 80% methanolic extract of Ludwigia octovalvis against Shiga toxin-producing E. coli O157:H7 in Balb/c mice following experimental infection

Preparation of Chlorophyll Nanoemulsion from Pomelo Leaves and Its Inhibition Effect on Melanoma Cells A375

Identification of Digestive Enzyme Inhibitors from Ludwigia octovalvis (Jacq.) P.H.Raven

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