Chloroquine Improves Deoxynivalenol-Induced Inflammatory Response and Intestinal Mucosal Damage in Piglets

Liao, Simeng; Tang, Shengguo; Tan, Bin; Li, Jianjun; Qi, Ming; Cui, Zhijuan; Zha, Andong; Wang, Yanan; Yin, Yulong; Sun, Peng; Tang, Yulong

doi:10.1155/2020/9834813

Cited by 15 publications

(7 citation statements)

References 36 publications

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“…In the present paper, the plasma concentrations of DAO and D-lactate were significant increased after piglets ingested DON-contaminated diet, indicating impaired intestinal barrier function. Similar findings were observed by other researchers [ 33 , 34 ]. Remarkably, supplementation of LGG into the DON-contaminated diet had lower plasma concentrations of D-lactate than that in the DON group and had no significant difference with CON group.…”

Section: Discussionsupporting

confidence: 93%

Lactobacillus rhamnosus GG ameliorates DON-induced intestinal damage depending on the enrichment of beneficial bacteria in weaned piglets

Bai

et al. 2022

J Animal Sci Biotechnol

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Background Deoxynivalenol (DON) is one of the most common environmental pollutants that induces intestinal inflammation and microbiota dysbiosis. Lactobacillus rhamnosus GG (LGG) is a probiotic that not only has anti-inflammatory effects, but also shows protective effect on the intestinal barrier. However, it is still unknown whether LGG exerts beneficial effects against DON-induced intestinal damage in piglets. In this work, a total of 36 weaned piglets were randomized to one of four treatment groups for 21 d. The treatment groups were CON (basal diet); LGG (basal diet supplemented with 1.77 × 1011 CFU/kg LGG); DON (DON-contaminated diet) and LGG + DON (DON-contaminated diet supplemented with 1.77 × 1011 CFU/kg LGG). Result Supplementation of LGG can enhance growth performance of piglets exposed to DON by improving intestinal barrier function. LGG has a mitigating effect on intestinal inflammation induced by DON exposure, largely through repression of the TLR4/NF-κB signaling pathway. Furthermore, supplementation of LGG increased the relative abundances of beneficial bacteria (e.g., Collinsella, Lactobacillus, Ruminococcus_torques_group and Anaerofustis), and decreased the relative abundances of harmful bacteria (e.g., Parabacteroides and Ruminiclostridium_6), and also promoted the production of SCFAs. Conclusions LGG ameliorates DON-induced intestinal damage, which may provide theoretical support for the application of LGG to alleviate the adverse effects induced by DON exposure.

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Section: Discussionsupporting

confidence: 93%

Lactobacillus rhamnosus GG ameliorates DON-induced intestinal damage depending on the enrichment of beneficial bacteria in weaned piglets

Bai

et al. 2022

J Animal Sci Biotechnol

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“…As we expected, RAPA alleviated inflammatory injury and downregulated the expressions of pyroptosis-related genes (NLRP3, ASC, caspase-1, and GSDMD) induced by FB 1 exposure in vivo and in vitro. Consistent with numerous reports, − we suggested that promoting autophagy can inhibit the pyroptosis pathway and reduce inflammation. However, it has also been manifested that autophagy can promote the activation of NLRP3 inflammasome and the release of IL-1β, − which may be attributed to the abnormal secretion pathways of inflammatory factors or the failure of autophagy to protect against pathogen-induced acute cellular stress.…”

Section: Discussionsupporting

confidence: 92%

mTOR-Mediated Autophagy Regulates Fumonisin B₁-Induced Intestinal Inflammation via Pyroptosis In Vivo and In Vitro

Liu

et al. 2022

J. Agric. Food Chem.

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Fumonisin B 1 (FB 1 ) is a fungal metabolite, which has an incremental detection rate in grains and feed worldwide. The nucleotide-binding oligomerization domain-like pyrin domain containing protein 3 (NLRP3) inflammasome is a critical element in pyroptosis activation, which participates in regulating enteritis. Meanwhile, autophagy is also engaged in intestinal inflammation. However, the function of pyroptosis and autophagy in FB 1 -mediated enterotoxicity remains unclear. In this study, we explored the effects of FB 1 on enteritis and the underlying mechanism in vivo and in vitro. Our data showed that FB 1 exposure damaged the intestinal epithelium and promoted the secretion of inflammatory cytokines. Meanwhile, FB 1 exposure significantly upregulated the expression of pyroptosis-related genes. Then, MCC950, an inhibitor of NLRP3, significantly blocked FB 1 -induced pyroptosis in IPEC-J2 cells. In addition, FB 1 treatment elevated the levels of autophagy. Moreover, the phosphorylation of the mammalian target of rapamycin (mTOR), an upstream protein of the autophagy pathway, was inhibited by FB 1 exposure. Notably, rapamycin, an inhibitor of mTOR, instead of MHY1485, an agonist of mTOR, could ameliorate FB 1 -induced intestinal inflammatory injury and inhibit the upregulation of pyroptosis-related genes. In summary, we demonstrated that autophagy exhibited a protective effect against NLRP3 inflammasome-dependent pyroptosis on FB 1 -induced enteritis. Our data clarify a favorable protective role for the activation of autophagy in FB 1 poisoning.

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“…A DON-contaminated diet usually affects growth performance by reducing the feed intake of piglets [30,31]. RES is a natural polyphenol belonging to the phytoalexin family.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Improves Intestinal Morphology and Anti-Oxidation Ability in Deoxynivalenol-Challenged Piglets

Hong

Lin

et al. 2022

Animals

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This study aimed to investigate the potential effects of resveratrol (RES) on intestinal function and oxidative stress in deoxynivalenol (DON)-challenged piglets. Twenty-four healthy Duroc × Yorkshire × Landrace weaned piglets at the age of 28 ± 1 days were randomly divided into four groups with six repetitions per group. The four groups were as follows: the control group (CON), fed with a basic diet; the RES group, fed with a basal diet + 300 mg/kg RES; the DON group, fed with a basal diet containing 2.65 mg/kg DON; and the DON + RES group, fed with a basal diet containing 2.65 mg/kg DON + 300 mg/kg RES. The results showed that the growth performance and intestinal function of DON-challenged piglets were significantly decreased (p < 0.05). Compared with the DON group, the average daily feed intake of piglets in the DON + RES group was significantly increased (p < 0.05). Additionally, dietary RES ameliorated DON-induced intestinal morphology impairment, as indicated by the increased (p < 0.05) jejunal villi height and the ratio of the jejunal villi height/crypt depth. Furthermore, after the addition of RES, the activities of superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in the jejunum mucosa were significantly increased, and the content of malondialdehyde (MDA) was significantly declined (p < 0.05). In addition, the level of reactive oxygen species (ROS) in the mitochondria was significantly reduced by RES, while the mitochondrial membrane potential in jejunum was significantly increased by RES (p < 0.05). However, there was no obvious difference between DON + RES and DON groups on average daily gain and the ratio of feed togain, except for the significant inhibition of average daily feed intake (p < 0.05). In conclusion, RES could effectively alleviate the DON-induced oxidative stress on weaned piglets, and reduce the damage to mitochondria and intestinal morphology, so as to improve the growth performance of piglets.

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Chloroquine Improves Deoxynivalenol-Induced Inflammatory Response and Intestinal Mucosal Damage in Piglets

Cited by 15 publications

References 36 publications

Lactobacillus rhamnosus GG ameliorates DON-induced intestinal damage depending on the enrichment of beneficial bacteria in weaned piglets

Lactobacillus rhamnosus GG ameliorates DON-induced intestinal damage depending on the enrichment of beneficial bacteria in weaned piglets

mTOR-Mediated Autophagy Regulates Fumonisin B₁-Induced Intestinal Inflammation via Pyroptosis In Vivo and In Vitro

Resveratrol Improves Intestinal Morphology and Anti-Oxidation Ability in Deoxynivalenol-Challenged Piglets

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Chloroquine Improves Deoxynivalenol-Induced Inflammatory Response and Intestinal Mucosal Damage in Piglets

Cited by 15 publications

References 36 publications

Lactobacillus rhamnosus GG ameliorates DON-induced intestinal damage depending on the enrichment of beneficial bacteria in weaned piglets

Lactobacillus rhamnosus GG ameliorates DON-induced intestinal damage depending on the enrichment of beneficial bacteria in weaned piglets

mTOR-Mediated Autophagy Regulates Fumonisin B1-Induced Intestinal Inflammation via Pyroptosis In Vivo and In Vitro

Resveratrol Improves Intestinal Morphology and Anti-Oxidation Ability in Deoxynivalenol-Challenged Piglets

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mTOR-Mediated Autophagy Regulates Fumonisin B₁-Induced Intestinal Inflammation via Pyroptosis In Vivo and In Vitro