Choledocho-choledochostomy: the natural history of healing in pigs

Laursen, H.; Thorsoe, H; Oxlund, Hans; Yasuda, Yoshikazu; Funch‐Jensen, Peter; Rokkjaer, M; Mortensen, Frank Viborg

doi:10.1007/s00534-006-1212-3

Cited by 4 publications

(2 citation statements)

References 7 publications

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“…In a pig model of common bile duct healing, the collagen content per volume showed an increase in collagen for the first 14 postoperative days (Laursen et al . ). The percentage of collagen area was used to evaluate changes in collagen content in benign biliary strictures in dogs (Geng et al .…”

Section: Discussionmentioning

confidence: 97%

Effect of tamoxifen on fibrosis, collagen content and transforming growth factor‐β1, ‐β2 and ‐β3 expression in common bile duct anastomosis of pigs

Siqueira

Oliveira

Carvalho

et al. 2017

Int J Experimental Path

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End-to-end anastomosis in the treatment for bile duct injury during laparoscopic cholecystectomy has been associated with stricture formation. The aim of this study was to experimentally investigate the effect of oral tamoxifen (tmx) treatment on fibrosis, collagen content and transforming growth factor-β1, -β2 and -β3 expression in common bile duct anastomosis of pigs. Twenty-six pigs were divided into three groups [sham (n = 8), control (n = 9) and tmx (n = 9)]. The common bile ducts were transected and anastomosed in the control and tmx groups. Tmx (40 mg/day) was administered orally to the tmx group, and the animals were euthanized after 60 days. Fibrosis was analysed by Masson's trichrome staining. Picrosirius red was used to quantify the total collagen content and collagen type I/III ratio. mRNA expression of transforming growth factor (TGF)-β1, -β2 and -β3 was quantified using real-time polymerase chain reaction (qRT-PCR). The control and study groups exhibited higher fibrosis than the sham group, and the study group showed lower fibrosis than the control group (P = 0.011). The control and tmx groups had higher total collagen content than the sham group (P = 0.003). The collagen type I/III ratio was higher in the control group than in the sham and tmx groups (P = 0.015). There were no significant differences in the mRNA expression of TGF-β1, -β2 and -β3 among the groups (P > 0.05). Tmx decreased fibrosis and prevented the change in collagen type I/III ratio caused by the procedure.

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Section: Discussionmentioning

confidence: 97%

Effect of tamoxifen on fibrosis, collagen content and transforming growth factor‐β1, ‐β2 and ‐β3 expression in common bile duct anastomosis of pigs

Siqueira

Oliveira

Carvalho

et al. 2017

Int J Experimental Path

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“…In a previous study we were able to establish that choledocho-choledochostomies, judged by breaking strength and collagen content, regained a stable level of strength 6 days after operation. 6 Accordingly, in the present study, we evaluated laparotomy in pigs on postoperative day 6. The evaluation revealed a signifi cantly increased breaking strength in T-tube-drained choledocho-choledochostomies.…”

Section: Discussionmentioning

confidence: 99%

T-tube drainage stimulates the healing of choledochocholedochostomies. An experimental study in pigs

Laursen

Kannerup

Oxlund

et al. 2008

J Hepatobiliary Pancreat Surg

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Hepatobiliäre Anastomosentechniken

Heidenhain

Rösch

Neumann

2010

Chirurg

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The success of hepatobiliary anastomoses is influenced by the diameter of the bile duct, the location within the biliary tract, the situation of primary or revision surgery and accompanying infections. The exact preoperative diagnostics of the anatomy of the biliary tract are indispensable for low complication rates. Within reconstructive surgery, hepaticojejunostomy has been established as the standard technique and a biliodigestive anastomosis is performed proximal to the cystic duct and 2-3 cm below the fork in the hepatic duct. In general, end-to-end anastomoses of the common bile duct are not recommended due to the high risk for stenosis. Within the liver hilus an exact preparation of all tubular structures is mandatory. With regard to possible perioperative complications operations on the hepatic duct or segmental bile ducts should be performed in specialized centers. Methods of drainage in hepatobiliary surgery are percutaneous transhepatic cholangiodrainage (PTCD), internal-external drainage, internal drainage with endoscopic or surgically placed stents, external-internal-external drainage and the T-drain.

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Choledocho-choledochostomy: the natural history of healing in pigs

Abstract: The present study on pigs shows that choledocho-choledochostomies, judged by breaking strength and collagen content, regain a stable level of strength 6 days after operation.

Cited by 4 publications

References 7 publications

Effect of tamoxifen on fibrosis, collagen content and transforming growth factor‐β1, ‐β2 and ‐β3 expression in common bile duct anastomosis of pigs

Effect of tamoxifen on fibrosis, collagen content and transforming growth factor‐β1, ‐β2 and ‐β3 expression in common bile duct anastomosis of pigs

T-tube drainage stimulates the healing of choledochocholedochostomies. An experimental study in pigs

Hepatobiliäre Anastomosentechniken

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