Cholestasis-associated reproductive toxicity in male and female rats: The fundamental role of mitochondrial impairment and oxidative stress

Ommati, Mohammad Mehdi; Farshad, Omid; Niknahad, Hossein; Arabnezhad, Mohammad Reza; Azarpira, Negar; Mohammadi, Hamidreza; Haghnegahdar, Maral; Mousavi, Khadijeh; Akrami, Shiva; Jamshidzadeh, Akram; Heidari, Reza

doi:10.1016/j.toxlet.2019.09.009

Cited by 55 publications

(31 citation statements)

References 66 publications

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“…Oxidative stress (OS), mitochondrial impairment, and their subsequent complications seem to play crucial roles in the xenobiotics-induced reprotoxicity and consequent infertility (20,21,(24)(25)(26)(27)(28)(29)(30). High sensitivity to OS, excessive reactive oxygen species (ROS) levels, and thiobarbituric acid reactive substances (TBARS) content could dramatically affect the functions of the reproductive organs (20,21,(26)(27)(28)(29)(30)(31)(32)(33)(34). For instance, oxidative injury and DNA damage studies indicated that high doses of MWCNT could cause DNA damage in murine sperm (35).…”

Section: Introductionmentioning

confidence: 99%

“…Cellular energy metabolism is pivotal in sperm forward motility (24,25,51), and there is ample evidence showing that xenobiotics could alter mitochondrial functionality and induce severe OS in different reproductive tissues (20,21,(27)(28)(29)(30). In this regard, Xu et al (52) reported that MWCNT caused severe damages to mitochondrial DNA in spermatocytes.…”

Section: Introductionmentioning

confidence: 99%

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Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

et al. 2020

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Carbon-based nanomaterials possess a remarkably high potential for biomedical applications due to their physical properties; however, their detrimental effects on reproduction are also concerned. Several reports indicate the toxicity of carbon nanotubes (CNT); nevertheless, their impact on intracellular organelles in the male reproductive organs has not been fully elucidated. Herein, we report on the reprotoxicity of single-walled (SWCNT) and multi-walled carbon nanotubes (MWCN) on several intracellular events and histological criteria in pubertal male BALB/c mice orally treated with 0, 10, and 50 mg/kg/day doses for 5 weeks. Biomarkers of oxidative stress and mitochondrial functionality, histopathological alterations, and epididymal sperm characteristics were determined. Oral administration of CNTs at 10 and 50 mg/kg evoked a significant decrement in weight coefficient, sperm viability and motility, hypo-osmotic swelling (HOS) test, sperm count, mitochondrial dehydrogenase activity, ATP content, total antioxidant capacity, and GSH/GSSH ratio in the testis and epididymal spermatozoa. On the other hand, percent abnormal sperm, testicular and sperm TBARS contents, protein carbonylation, ROS formation, oxidized glutathione level, and sperm mitochondrial depolarization were considerably increased. Significant histopathological and stereological alterations in the testis occurred in the groups challenged with CNTs. The current findings indicated that oxidative stress and mitochondrial impairment might substantially impact CNTs-induced reproductive system injury and sperm toxicity. The results can also be used to establish environmental standards for CNT consumption by mammals, produce new chemicals for controlling the rodent populations, and develop therapeutic approaches against CNTs-associated reproductive anomalies in the males exposed daily to these nanoparticles.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

et al. 2020

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“…The oxidative damage of proteins was assessed by the determination of carbonyl groups based on the reaction with dinitrophenylhydrazine (DNPH) [ 60 , 61 ]. Briefly, 100 µl of TCA (20% w/v, 4ºC) was added to 1 ml of the heart tissue homogenate and centrifuged (700 g, 15 min).…”

Section: Methodsmentioning

confidence: 99%

Taurine mitigates cirrhosis-associated heart injury through mitochondrial-dependent and antioxidative mechanisms

Mousavi¹,

Niknahad²,

Mostofi³

et al. 2020

ceh

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“…2′, 7′-dichlorofluorescein-diacetate (DCF-DA) was used as a fluorescent probe to estimate brain ROS level. [29][30][31] Brain tissue was weighed in different groups, with a ratio of 1:10 and was homogenized in 5 mL of ice-cooled (4°C) Tris-HCl buffer (40 mM, pH = 7.4). Samples (100 μL) of the resulted tissue homogenate were mixed with Tris-HCl buffer (1 mL; pH = 7.4 4°C) and 5 μL of DCF-DA (Final concentration of 10 μM).…”

Section: Reactive Oxygen Species (Ros) Formation In the Brain Tissuementioning

confidence: 99%

<p>The Potential Neuroprotective Role of Citicoline in Hepatic Encephalopathy</p>

Farshad

Keshavarz

Heidari

et al. 2020

JEP

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Cholestasis-associated reproductive toxicity in male and female rats: The fundamental role of mitochondrial impairment and oxidative stress

Cited by 55 publications

References 66 publications

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

Taurine mitigates cirrhosis-associated heart injury through mitochondrial-dependent and antioxidative mechanisms

<p>The Potential Neuroprotective Role of Citicoline in Hepatic Encephalopathy</p>

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