Cholesterol and Hematopoietic Stem Cells: Inflammatory Mediators of Atherosclerosis

Lang, Jennifer K.; Cimato, Thomas R.

doi:10.5966/sctm.2013-0205

Cited by 20 publications

(17 citation statements)

References 27 publications

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“…Small animal model studies clearly show the effects of disease on the levels of HSC and HSPC populations including myocardial infarction , stroke , hypercholesterolemia , aging , and cancer . In human subjects, CD34+ Lineage‐ CD45dim cells in the blood stream correlate with total cholesterol levels , consistent with studies in small animal models of hypercholesterolemia.…”

supporting

confidence: 70%

“…However, more precise distinction of the subtypes of CD34+ cells in the blood stream of humans has not been performed. Mechanistically, HSCs and HSPCs play key roles in progression of atherosclerosis and cancer in animal models by contributing to regeneration of myeloid lineage cells, monocytes, and neutrophils among others. Development of methods to measure HSCs and HSPCs in venous blood of human subjects would be a key advance to realize translation of animal model findings to convenient measurements in patients with disease, avoiding or supplementing information from bone marrow samples.…”

mentioning

confidence: 99%

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Simultaneous measurement of human hematopoietic stem and progenitor cells in blood using multicolor flow cytometry

Cimato

Furlage

Conway

et al. 2016

Cytometry Part B Clinical

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Hematopoietic stem cells are the source of all inflammatory cell types. Discovery of specific cell surface markers unique to human hematopoietic stem (HSC) and progenitor (HSPC) cell populations has facilitated studies of their development from stem cells to mature cells. The specific marker profiles of HSCs and HSPCs can be used to understand their role in human inflammatory diseases. The goal of this study is to simultaneously measure HSCs and HSPCs in normal human venous blood using multi-color flow cytometry. Our secondary aim is to determine how G-CSF mobilization alters the quantity of each HSC and HSPC population. Here we show that cells within the CD34+ fraction of human venous blood contains cells with the same cell surface markers found in human bone marrow samples. Mobilization with G-CSF significantly increases the quantity of total CD34+ cells, blood borne HSCs, multipotent progenitors, common myeloid progenitors, and megakaryocyte erythroid progenitors as a percentage of total MNCs analyzed. The increase in blood borne common lymphoid and granulocyte macrophage progenitors with G-CSF treatment did not reach significance. G-CSF treatment predominantly increased the numbers of HSCs and multipotent progenitors in the total CD34+ cell population; common myeloid progenitors and megakaryocyte erythroid progenitors were enriched relative to total MNCs analyzed, but not relative to total CD34+ cells. Our findings illustrate the utility of multi-color flow cytometry to quantify circulating HSCs and HSPCs in venous blood samples from human subjects.

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supporting

confidence: 70%

mentioning

confidence: 99%

Simultaneous measurement of human hematopoietic stem and progenitor cells in blood using multicolor flow cytometry

Cimato

Furlage

Conway

et al. 2016

Cytometry Part B Clinical

Self Cite

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show abstract

“…21 IL-6 and TNF-a are classic proinflammatory cytokines to promote AS, whereas IL-4 is an anti-inflammatory cytokine. 22 It appears to be a promising strategy for AS treatment by reducing circulating IL-6 and TNF-a.…”

Section: Discussionmentioning

confidence: 99%

Metformin ameliorates the proinflammatory state in patients with carotid artery atherosclerosis through sirtuin 1 induction

Deng

Yang

et al. 2015

Translational Research

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“…This is consistent with recent work from our lab indicating that DIO promotes colonic Lgr5 + stem cell expansion during cancer initiation (DeClercq et al, 2015). Interestingly, the inactivation of cholesterol efflux transporters (ABCA1 and ABCG1) increase the number of circulating hematopoietic stem cells (HSPCs) (Feng et al, 2012; Gomes et al, 2010; Yvan-Charvet et al, 2007) and activate inflammatory cells (Lang and Cimato, 2014), suggesting that the accumulation of cellular membrane cholesterol may directly regulate aspects of stem cell biology. From a mechanistic perspective, the Wnt/β-catenin pathway maintains a stem cell/progenitor phenotype in many cell types (Pinto and Clevers, 2005).…”

Section: Membrane Cholesterol and Stem Cellsmentioning

confidence: 99%

Omega-3 fatty acids, membrane remodeling and cancer prevention

Fuentes

Kim

Yang

et al. 2018

Molecular Aspects of Medicine

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Proteins are often credited as the macromolecule responsible for performing critical cellular functions, however lipids have recently garnered more attention as our understanding of their role in cell function and human health becomes more apparent. Although cellular membranes are the lipid environment in which many proteins function, it is now apparent that protein and lipid assemblies can be organized to form distinct micro- or nanodomains that facilitate signaling events. Indeed, it is now appreciated that cellular function is partly regulated by the specific spatiotemporal lipid composition of the membrane, down to the nanosecond and nanometer scale. Furthermore, membrane composition is altered during human disease processes such as cancer and obesity. For example, an increased rate of lipid/cholesterol synthesis in cancerous tissues has long been recognized as an important aspect of the rewired metabolism of transformed cells. However, the contribution of lipids/cholesterol to cellular function in disease models is not yet fully understood. Furthermore, an important consideration in regard to human health is that diet is a major modulator of cell membrane composition. This can occur directly through incorporation of membrane substrates, such as fatty acids, e.g., n-3 polyunsaturated fatty acids (n-3 PUFA) and cholesterol. In this review, we describe scenarios in which changes in membrane composition impact human health. Particular focus is placed on the importance of intrinsic lipid/cholesterol biosynthesis and metabolism and extrinsic dietary modification in cancer and its effect on plasma membrane properties.

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Cholesterol and Hematopoietic Stem Cells: Inflammatory Mediators of Atherosclerosis

Cited by 20 publications

References 27 publications

Simultaneous measurement of human hematopoietic stem and progenitor cells in blood using multicolor flow cytometry

Simultaneous measurement of human hematopoietic stem and progenitor cells in blood using multicolor flow cytometry

Metformin ameliorates the proinflammatory state in patients with carotid artery atherosclerosis through sirtuin 1 induction

Omega-3 fatty acids, membrane remodeling and cancer prevention

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