Choline and betaine ameliorate liver lipid accumulation induced by vitamin B6 deficiency in rats

Kitagawa, Erina; Yamamoto, Tatsuya; Fujishita, Mayuko; Ota, Yuki; Yamamoto, Kohei; Nakagawa, Tomoyuki; Hayakawa, T.

doi:10.1080/09168451.2016.1240604

Cited by 12 publications

(7 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Experimental studies have proved that choline deficiency might cause varying degrees of liver diseases ranging from dyslipidemia to liver cancer 27 , whereas choline or betaine supplementation could ameliorate liver damage 15,16 . Second, though choline is essential for maintaining normal liver function, its functions are more than this.…”

Section: Discussionmentioning

confidence: 99%

“…Particularly, choline is a major source of methyl groups via oxidation to betaine, their functions involving the re-methylation of homocysteine to methionine are critical for DNA and histone methylation homeostasis, and therefore have been reported to be associated with cancer risk 2 (including liver cancer) and survival [7][8][9][10] . Experimental studies have reported that when deprived of choline, varying degrees of liver damages and liver diseases developed, including elevated transaminases 11 , affected lipid metabolism and transport 12 , fatty liver 13 , liver cirrhosis 13 and even liver cancer 14 , whereas choline or betaine supplementation ameliorated liver damage 15,16 , and choline supplementation increased global DNA methylation and DNA methyltransferase expression in HepG2 cells 17 , which implied that choline or betaine might not only be associated with hepatocarcinogenesis, but also with liver cancer survival. To date, although scarcely, three case control studies [18][19][20] have evaluated the relationship between choline/betaine and human liver cancer risk, in which consistently favorable effects of choline were found.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Serum choline is associated with hepatocellular carcinoma survival: a prospective cohort study

Liu¹,

Yishake²,

Fang³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Higher choline/betaine levels have been linked to lower risk of liver cancer, whereas existing data in relation to hepatocellular carcinoma (HCC) prognosis are scarce. Our objective was to examine the associations of the serum choline and betaine with HCC survival. Methods: 866 newly diagnosed HCC patients were enrolled in the Guangdong Liver Cancer Cohort. Serum choline and betaine were assessed using high-performance liquid chromatography with online electro-spray ionization tandem mass spectrometry. Liver cancer-specific survival (LCSS) and overall survival (OS) were calculated. Cox proportional hazards models were used to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs). Results: Both LCSS (T3 vs. T1: HR=0.69, 95% CI: 0.51-0.94; P-trend <0.05) and OS (T3 vs. T1: HR=0.73, 95% CI: 0.54-0.99; P-trend <0.05) were better with sex-specific tertiles of serum choline levels. The associations were not significantly modified across strata of selected prognostic factors, except in the different C-reactive protein (CRP) levels, the favorable associations between serum choline and LCSS and OS were only existed among patients with CRP ≥3.0 mg/L. No significant associations were found between serum betaine levels and either LCSS or OS. Conclusions: This study revealed that higher serum choline levels were associated with better HCC survival, especially in HCC patients with systemic inflammation status. No significant associations were found between serum betaine and HCC survival. Our finding might open new prospect in understanding the benefits of choline on HCC survival. Registration: The Guangdong Liver Cancer Cohort was registered at clinicaltrials.gov as NCT 03297255.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Serum choline is associated with hepatocellular carcinoma survival: a prospective cohort study

Liu¹,

Yishake²,

Fang³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Liver is among the first organs to accumulate choline absorbed from the intestine and responsible for most of choline metabolism [26]. Experimental studies have proved that choline deficiency might cause varying degrees of liver diseases ranging from dyslipidemia to liver cancer [26], whereas choline or betaine supplementation could ameliorate liver damage [15,16]. Second, though choline is essential for maintaining normal liver function, its functions are more than this.…”

Section: Discussionmentioning

confidence: 99%

“…Particularly, choline is a major source of methyl groups via oxidation to betaine, their functions involving the re-methylation of homocysteine to methionine are critical for DNA and histone methylation homeostasis, and therefore have been reported to be associated with cancer risk [2] (including liver cancer) and survival [7][8][9][10]. Experimental studies have reported that when deprived of choline, varying degrees of liver damage and liver diseases developed, including elevated transaminases [11], affected lipid metabolism and transport [12], fatty liver [13], liver cirrhosis [13] and even liver cancer [14], whereas choline or betaine supplementation ameliorated liver damage [15,16], and choline supplementation increased global DNA methylation and DNA methyltransferase expression in HepG2 cells [17], which implied that choline or betaine might not only be associated with hepatocarcinogenesis, but also with liver cancer survival. To date, although scarcely, three case control studies [18][19][20] have evaluated the relationship between choline and betaine and human liver cancer risk, in which consistently favorable effects of choline were found.…”

Section: Introductionmentioning

confidence: 99%

Serum choline is associated with hepatocellular carcinoma survival: a prospective cohort study

et al. 2020

View full text Add to dashboard Cite

Background: Higher choline and betaine levels have been linked to lower risk of liver cancer, whereas existing data in relation to hepatocellular carcinoma (HCC) prognosis are scarce. Our objective was to examine the associations of the serum choline and betaine with HCC survival. Methods: 866 newly diagnosed HCC patients were enrolled in the Guangdong Liver Cancer Cohort. Serum choline and betaine were assessed using high-performance liquid chromatography with online electro-spray ionization tandem mass spectrometry. Liver cancer-specific survival (LCSS) and overall survival (OS) were calculated. Cox proportional hazards models were used to estimate the hazard ratios (HRs) and 95% confidence intervals (CIs). Results: Serum choline levels were associated with better LCSS (T3 vs. T1: HR = 0.69, 95% CI: 0.51-0.94; P-trend < 0.05) and OS (T3 vs. T1: HR = 0.73, 95% CI: 0.54-0.99; P-trend < 0.05). The associations were significantly modified by C-reactive protein (CRP) levels but not by other selected prognostic factors including sex, age, etc. The favorable associations between serum choline and LCSS and OS were only existed among patients with CRP ≥3.0 mg/L. No significant associations were found between serum betaine levels and either LCSS or OS. Conclusions: This study revealed that higher serum choline levels were associated with better HCC survival, especially in HCC patients with systemic inflammation status. No significant associations were found between serum betaine and HCC survival. Our findings suggest the benefits of choline on HCC survival. Trial registration: The Guangdong Liver Cancer Cohort was registered at clinicaltrials.gov as NCT03297255.

show abstract

“…We previously revealed that PC, choline, and betaine ameliorate liver lipid accumulation in rats fed a 0.9% Met-supplemented B6-deficient diet (2,3). Choline and betaine are anti-obesity factors (16)(17)(18)(19) linked to Met metabolism, and betaine is produced by choline oxidation (20).…”

mentioning

confidence: 99%

Accumulation of Liver Lipids Induced by Vitamin B<sub>6 </sub>Deficiency Was Effectively Ameliorated by Choline and, to a Lesser Extent, Betaine

Kitagawa

Ota

Hasegawa

et al. 2019

J Nutr Sci Vitaminol

Self Cite

View full text Add to dashboard Cite

Despite previous studies suggesting that choline and betaine ameliorate lipid accumulation in rat livers, the relative effectiveness of the two nutrients is unclear. We examined the efficacy of dietary supplementation with choline or betaine in ameliorating lipid accumulation induced by vitamin B 6 (B6) deficiency in the rat liver. Male Wistar rats were fed control, B6-deficient, choline-supplemented B6-deficient, betaine-supplemented B6-deficient, or both choline and betaine-supplemented B6-deficient diets (all containing 9 g of l-methionine (Met)/kg) for 35 d. Two experiments were performed, i.e., one using 17 mmol/kg diet choline bitartrate, betaine anhydrous, and the combination and another using 8.5 mmol/ kg diet. Rats fed a B6-deficient diet developed lipid accumulation in the liver with a reduction of plasma lipids induced by the disruption of Met metabolism. However, the addition of 17 mmol/kg diet choline or betaine was sufficient to ameliorate the disruptions of lipid and Met metabolism. Additionally, 8.5 mmol/kg diet choline ameliorated liver lipid deposition, while the same amount of betaine had no significant effects on liver or plasma lipid profiles. Supplementation with choline resulted in a higher liver betaine than that found using the same amount of betaine alone, although the overall liver betaine content was reduced in B6-deficient rats. Our findings indicate that choline is more effective than betaine in ameliorating B6 deficiency-related disruptions in Met metabolism and liver lipid accumulation by increasing liver betaine levels.

show abstract

Choline and betaine ameliorate liver lipid accumulation induced by vitamin B6 deficiency in rats

Cited by 12 publications

References 34 publications

Serum choline is associated with hepatocellular carcinoma survival: a prospective cohort study

Serum choline is associated with hepatocellular carcinoma survival: a prospective cohort study

Serum choline is associated with hepatocellular carcinoma survival: a prospective cohort study

Accumulation of Liver Lipids Induced by Vitamin B<sub>6 </sub>Deficiency Was Effectively Ameliorated by Choline and, to a Lesser Extent, Betaine

Contact Info

Product

Resources

About