Choline esterase inhibitory properties of alkaloids from two Nigerian Crinum species

Houghton, Peter J.; Agbedahunsi, Joseph M.; Adegbulugbe, Aderonke

doi:10.1016/j.phytochem.2004.08.052

Cited by 84 publications

(56 citation statements)

References 15 publications

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“…Phytochemical analysis of such plants led to the characterization of cyanotroside from Cynanchum atratum and zeathin from Fiatoua villosa as potent AChE inhibitors (Lee et al, 2004, Heo et al, 2002. Houghton et al 2004 has reported the cholinesterase inhibitory activity of two Nigerian Crinum species i.e. Crinum jagus and Crinum glaucum, which could justify their use as memory enhancers.…”

Section: Introductionmentioning

confidence: 99%

Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some selected Nigerian medicinal plants

Elufioye¹,

Obuotor²,

Sennuga³

et al. 2010

Rev. bras. farmacogn.

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RESUMO: "Atividade inibitória da acetilcolinesterase e butirilcolinesterase de algumas plantas medicinais da Nigéria". As plantas podem ser úteis para estimular a memória, bem como serem usadas para combater o envelhecimento. Vinte e duas plantas, de dezesseis famílias, foram investigadas in vitro oara verificar sua atividade inibidora das enzimas acetilcolinesterase (AChE) e butirilcolinesterase (BuChE) pelo método espectrofotométrico de Ellman in situ e métodos de bioautografia utilizando fisostigmina como padrão. Pelo menos três partes morfológicas de cada planta foram analisadas e a concentração de ensaio foi de 42,5 µg/mL. Algumas plantas foram ativas em ambas as enzimas, embora com algumas partes mais ativas que outras. A casca da raiz de Spondias mombin apresentou a maior atividade as duas enzimas, 64,77% para AChE e 83,94% para BuChE. Outras partes das plantas selecionadas apresentaram boa seletividade em suas ações. As plantas seletivamente ativos contra AChE foram as casca do caule e casca da raiz de Alchornia laxiflora (41,12%), e casca da raiz de Callophyllum inophyllurn (56,52%). As folhas de C. jagus (74,25%), folhas de Morinda lucida (40,15%), folhas e casca do caule de Peltophorum pterocarpum (49,5% e 68,85%, respectivamente), physiostigmine inibiu 90,31%. Em geral, atividades melhoras foram apresentadas contra BuChE. Folhas, casca da raiz e casca do caule Bombax bromoposenze foram particularmente ativos. A inibição foi acima de 80%. Outras partes de algumas espécies também foram seletivas, como as partes aéreas de Antiaris africana, Cissampelos owarensis (78,96%), folhas e casca do caule de Combretum molle (90,42% e 88,13%, respectivamente), casca da raiz e de tubérculos de Dioscorea dumentorum (mais de 87%), folhas de G cola, cascas de raiz de Markhamia tomentosa, casca do caule de Pycnanthus angolensis e folhas de Tetrapleura tetraptera. A maioria destas plantas são utilizadas como alimentos ou ingredientes alimentares na Nigéria e podem ser responsáveis pela baixa incidência da doença de Alzheimer no país e desempenhar determinadas funções na mediação da doença.Unitermos: Plantas medicinais, acetilcolinesterase, butrilcolinesterase, perda da memória. ABSTRACT:Plants have been found to be useful as memory enhansers as well as antiaging. Twenty two of such plants from sixteen families were investigated for their acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory activities using the in vitro Ellman's spectrophotometric and in situ bioautographic methods with physostigmine as standard. At least three morphological parts were examined for each of the plants investigated and the test concentration was 42.5 μg/ mL. Some plants were active on both enzymes though with some morphological parts being more active than others. The root bark of Spondias mombin showed the highest activity to the two enzymes; 64.77% and 83.94% on AChE and BuChE respectively. Other plant parts of the selected plants exhibited some remarkable selectivity in their actions. Those selectively active against...

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Section: Introductionmentioning

confidence: 99%

Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some selected Nigerian medicinal plants

Elufioye¹,

Obuotor²,

Sennuga³

et al. 2010

Rev. bras. farmacogn.

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“…Among the plant families that have been examined for their AChE inhibitory activity are: Amaryllidaceae (Houghton et al 2004, Rhee et al 2004, Boraginaceae (Ahmad et al 2003b), Chenopodiaceae (Ferheen et al 2005), Lamiaceae (Ahmad et al 2005), Liliaceae (Atta-urRahman et al 2002), and Solanaceae (Roddick 1989.…”

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confidence: 99%

In vitro inhibition of acetylcholinesterase by crude plant extracts from Colombian flora

Niño

Hernández

Correa

et al. 2006

Mem. Inst. Oswaldo Cruz

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The methanol extracts from five different plant families (Asteraceae, Euphorbiaceae, Melastomataceae, Rubiaceae, and Solanaceae) collected at Regional Natural Park Ucumarí (Colombia), were screened for their acetylcholinesterase inhibitory activity through the modified Ellman's spectrophotometric method. The best inhibitory activities on this study were shown by the extracts of Solanum leucocarpum Dunal (IC 50 = 204.59 mg/l) and Witheringia coccoloboides (Damm) (IC 50 = 220.68 mg/l), both plants belonging to the Solanaceae family.Key words: acetylcholinesterase (AChE) inhibitors -Asteraceae -Euphorbiaceae -Melastomataceae -Rubiaceae -SolanaceaeThe acetylcholinesterase enzyme (AChE) is an attractive target for the rational drug design and for the discovery of mechanism based inhibitors because of its role in the hydrolysis of the neurotransmitter acetylcholine (ACh). AChE inhibitors are the most effective approach to treat the cognitive symptoms of Alzheimer disease (AD) (Kalauni et al. 2002) and other possible therapeutic applications in the treatment of Parkinson's disease, senile dementia, and ataxia, among others (Ahmad et al. 2003a). AChE inhibitors as eserine, tacrine, donepezil, rivastigmine, and galanthamine are the only drugs currently approved for the treatment of AD; however, these drugs are known to have limitations for clinical use due to their short-half-lives and/or unfavorable side-effects (Sung et al. 2002).Because the search of plant extracts that selectively inhibit AChE is of paramount importance to find novel and more potent AChE inhibitors, many of them have been screened and as consequence there have been found extracts and isolated pure compounds with AChE and/or butyrylcholinesterase (BChE) inhibitory properties. Among the plant families that have been examined for their AChE inhibitory activity are: Amaryllidaceae Continuing with the study of the flora from Regional Natural Park Ucumarí (RNPU, Risaralda, Colombia) as a source of new secondary metabolites with diverse grade of biological activities (Niño et al. 2003, 2006, Mosquera et al. 2004a) and the fact that there still is great interest in finding novel and better AChE inhibitors, prompted us to screen 27 crude methanol extracts belonging to the Asteraceae, Euphorbiaceae, Melastomataceae, Rubiaceae, and Solananceae families for their AChE inhibitory activities. MATERIALS AND METHODSIn this study, the solvent hexanes, dichloromethane, and methanol (analytical-reagent grade) were purchased from Mallinckrodt (Phillipsburg, NJ, US). Silica gel plates (Silica gel 60 F 254 0.2 mm layer thickness) were purchased from Merck (Darmstadt, Germany). The electric eel acetylcholinesterase Sigma (St Louis, MO, US) was used. Acetylthiocholine iodide and 5,5-dithiobis (2-nitro benzoic acid) (DTNB) were purchased from Aldrich (St Louis, MO, US). A Spectronic Genesys 5 spectrophotometer Milton Roy (Rochester, NY, US) was used for all measurements of acetylcholinesterase inhibitory activity.Plant material -Twenty seven plants from the selected famili...

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“…The acetylcholinesterase inhibitory activity of active extracts was scored according to the appearance of a white spot on the purple background of the TLC plate as: weakly active (+), moderately active (++), strongly active (+++). From the 57 plants studied, eight Houghton et al (2004). Moreover, the AChE inhibitory activity of other species of Hippeastrum has been studied previously by Giordani et al (2008).…”

Section: Resultsmentioning

confidence: 99%

“…Acetylcholinesterase activity of the alkaloids hamayne, crinamine, lycorine, pseudolycorine, 6-hydroxycrinamine, and haemanthamine from Crinum jagus (Thomps.) Dandy has been reported by Houghton et al (2004).…”

Section: Discussionmentioning

confidence: 99%

Screening of plants of Amaryllidaceae and related families from Panama as sources of acetylcholinesterase inhibitors

Calderón

Cubilla

Espinosa

et al. 2010

Pharmaceutical Biology

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Choline esterase inhibitory properties of alkaloids from two Nigerian Crinum species

Cited by 84 publications

References 15 publications

Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some selected Nigerian medicinal plants

Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some selected Nigerian medicinal plants

In vitro inhibition of acetylcholinesterase by crude plant extracts from Colombian flora

Screening of plants of Amaryllidaceae and related families from Panama as sources of acetylcholinesterase inhibitors

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