Chondrogenesis and hypertrophy in response to aggregate behaviors of human mesenchymal stem cells on a dendrimer-immobilized surface

Wongin, Sopita; Ogawa, Yuuki; Kim, Mee‐Hae; Viravaidya-Pasuwat, Kwanchanok; Kino‐oka, Masahiro

doi:10.1007/s10529-017-2339-9

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…Based on the Live/Dead® cell staining in the present work, the cells seeded in βTFS‐Alg formed small clusters and appeared to have poor adhesion to the scaffold. Regardless of cell type, many studies have shown that differentiation of cartilage cells is promoted by aggregation 29–31 . Therefore, the uneven distribution of cells in our scaffolds may promote cartilage differentiation.…”

Section: Discussionmentioning

confidence: 98%

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Evaluation of alginate‐coated β‐tricalcium phosphate fiber scaffold for cell culture

Kawamura,

Furuya,

Sasaki

et al. 2024

J Biomed Mater Res

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Ex vivo tissue engineering is an effective therapeutic approach for the treatment of severe cartilage diseases that require tissue replenishment or replacement. This strategy demands scaffolds that are durable enough for long‐term cell culture to form artificial tissue. Additionally, such scaffolds must be biocompatible to prevent the transplanted matrix from taking a toll on the patient's body. From the viewpoint of structure and bio‐absorbability, a β‐tricalcium phosphate (β‐TCP) fiber scaffold (βTFS) is expected to serve as a good scaffold for tissue engineering. However, the fragility and high solubility of β‐TCP fibers make this matrix unsuitable for long‐term cell culture. To solve this problem, we developed an alginate‐coated β‐TCP fiber scaffold (βTFS‐Alg). To assess cell proliferation and differentiation in the presence of βTFS‐Alg, we characterized ATDC5 cells, a chondrocyte‐like cell line, when grown in this matrix. We found that alginate coated the surface of βTFS fiber and suppressed the elution of Ca2+ from β‐TCP fibers. Due to the decreased solubility of βTFS‐Alg compared with β‐TCP, the former provided an improved scaffold for long‐term cell culture. Additionally, we observed superior cell proliferation and upregulation of chondrogenesis marker genes in ATDC5 cells cultured in βTFS‐Alg. These results suggest that βTFS‐Alg is suitable for application in tissue culture.

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Section: Discussionmentioning

confidence: 98%

“…Regardless of cell type, many studies have shown that differentiation of cartilage cells is promoted by aggregation. [29][30][31] Therefore, the uneven distribution of cells in our scaffolds may promote cartilage differentiation. Additionally, although calcium alginate forms a stable hydrogel, sodium alginate is soluble in water.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of alginate‐coated β‐tricalcium phosphate fiber scaffold for cell culture

Kawamura,

Furuya,

Sasaki

et al. 2024

J Biomed Mater Res

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Fast fabrication of stable cartilage-like tissue using collagen hydrogel microsphere culture

Liu

Chen

et al. 2017

J. Mater. Chem. B

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Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis

Nabizadeh¹,

Nasrollahzadeh²,

Daemi³

et al. 2022

Beilstein J. Nanotechnol.

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Osteoarthritis, which typically arises from aging, traumatic injury, or obesity, is the most common form of arthritis, which usually leads to malfunction of the joints and requires medical interventions due to the poor self-healing capacity of articular cartilage. However, currently used medical treatment modalities have reported, at least in part, disappointing and frustrating results for patients with osteoarthritis. Recent progress in the design and fabrication of tissue-engineered microscale/nanoscale platforms, which arises from the convergence of stem cell research and nanotechnology methods, has shown promising results in the administration of new and efficient options for treating osteochondral lesions. This paper presents an overview of the recent advances in osteochondral tissue engineering resulting from the application of micro- and nanotechnology approaches in the structure of biomaterials, including biological and microscale/nanoscale topographical cues, microspheres, nanoparticles, nanofibers, and nanotubes.

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Chondrogenesis and hypertrophy in response to aggregate behaviors of human mesenchymal stem cells on a dendrimer-immobilized surface

Abstract: Incubation of hMSCs affects the expression of type II and X collagens via effects on cell aggregate behavior and stress fiber formation.

Cited by 3 publications

References 20 publications

Evaluation of alginate‐coated β‐tricalcium phosphate fiber scaffold for cell culture

Evaluation of alginate‐coated β‐tricalcium phosphate fiber scaffold for cell culture

Fast fabrication of stable cartilage-like tissue using collagen hydrogel microsphere culture

Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis

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