Fast fabrication of stable cartilage-like tissue using collagen hydrogel microsphere culture

Abstract

Fabrication of cartilage-like tissue by mimicking chondrogenesis of MSCs in collagen hydrogel microsphere (CHM) culture.

Dynamic mechanical loading facilitated chondrogenic differentiation of rabbit BMSCs in collagen scaffolds

“…5), suggesting that dynamic loading contributed to the chondrogenic differentiation of BMSCs. COL1A2 is a maker related to cell adhesion, migration and osteogenesis [35]. Interestingly, the COL1A2 gene expression in dynamic culture group was remarkedly high at Day 7 and gradually decreased with time extension.…”

Bionic cartilage acellular matrix microspheres as a scaffold for engineering cartilage

“…Based on our previous study, bionic cartilage microspheres could be made in vitro within 15 days and the chondrogenic genes expression was stable. 23 Combining the collagen microspheres culture system and the microsphere decellularization technique, we had managed to fabricate three ECM scaffolds (5-BCAMM, 10-BCAMM, and 15-BCAMM) from allogeneic MSCs, and conducted a comprehensive evaluation to their chondrogenic inductivities. To avoid any potential chondrogenic effect from cell culture medium and to mimic the limited nutrient microenvironment in the natural articular cartilage, we used the basic culture medium without any FBS that may contain extra nutrients and various unknown cytokines.…”

“…In our previous study, we found that the chondrogenesis of MSCs in three-dimensional (3D) collagen hydrogel microsphere (CHM) culture system could partly mimic the natural process of cartilage formation, especially for the cell migration, condensation, abundant cartilage specific matrix secretion and low hypertrophic gene expression. 23 The cartilage-like tissue formed from above culture were named bionic cartilage microspheres (BCM), which contained the homogeneous cartilage specific matrix, including glycosaminoglycans (GAGs) and type II collagen.…”

ChondroGELesis: Hydrogels to harness the chondrogenic potential of stem cells

“…Indeed, chondrogenesis was enhanced when 10 x 10 6 million periosteum-derived stem cells/mL were seeded in collagen gels as microaggregates compared to single cells (174). Similarly, rabbit MSC chondrogenesis was increased when they were encapsulated as microspheres in collagen gels, compared to bulk, single cell encapsulation, or non-encapsulated cell pellets (175). Žigon-Branc et al also found that soft 538 Pa GelMA gels enhanced human ASC microspheroid chondrogenesis, compared to stiffer 3584 or 7263 Pa gels (25).…”