Choroidal Microvascular Repair after Argon Laser Photocoagulation

Perry, Dwight D.; Risco, Jose Miguel

doi:10.1016/0002-9394(82)90475-5

Cited by 46 publications

(23 citation statements)

References 8 publications

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“…Using the corrosion cast method, many authors have described the effects of laser photocoagulation on the choroid [15][16][17][18][19] and on experimental CNV [5,[20][21][22][23]. However, most of them studied CNV at some time points, and sequential morphological characteristics of CNV are unclear.…”

Section: Discussionmentioning

confidence: 99%

“…However, most of them studied CNV at some time points, and sequential morphological characteristics of CNV are unclear. Moreover, most of them studied large animals, such as monkeys [5,17,[19][20][21][22][23], cats [15,16] Kuroki/Bhutto/Kitaoka/Amemiya [18]. Small animals must also be considered as experimental models because they are easy to examine and treat.…”

Section: Discussionmentioning

confidence: 99%

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Natural Course of Experimental Choroidal Neovascularization: Three-Dimensional Study with Corrosion Cast and Scanning Electron Microscope

et al. 2002

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Objectives: The details of the morphological features of choroidal neovascularization (CNV) remain unclear. The purpose of this study was to establish a CNV rat model and study the natural course of CNV using vascular casts and a scanning electron microscope (SEM). Methods: Focal laser photocoagulation (argon green 50 mW, 0.04 s, 200 µm) was applied to Brown Norway pigmented rats. Choroidal vascular casts were prepared 1 and 3 days, 1 and 2 weeks, and 1, 3 and 6 months after laser photocoagulation. The choroidal casts were examined with a SEM. Results: One day after photocoagulation, corrosion casts and SEM revealed complete defects of the choriocapillaris at the laser shot sites. One week after photocoagulation, small vascular buds originating from the damaged choriocapillaris were observed. Two weeks after photocoagulation, newly formed CNV originating from an individual laser burn was observed. One to three months after photocoagulation, these new vessels were connected to each other to form CNV networks. Six months later, some thin and atrophic vessels were observed in the CNV network. Conclusions: We succeeded in making fine corrosion casts of CNV formed by photocoagulation in pigmented rats and in demonstrating the details of CNV formation and regression. It is hoped that the results of this study will contribute to the development of a drug therapy for CNV and to the interpretation of diagnostic imaging of CNV in humans.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Natural Course of Experimental Choroidal Neovascularization: Three-Dimensional Study with Corrosion Cast and Scanning Electron Microscope

et al. 2002

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“…The development of the laser-induced CNV model, which has had a significant impact on our understanding of the mechanism and treatment of CNV, has become the most widely used model in wet AMD research. 39) Since many animal models, including monkey, 39,40) rabbits, 41) cats 42) and rats, 43,44) have been developed and used to evaluate the effect of various antiangiogenic agents on CNV. Among the numerous animal models, the pigmented rats have been used by several groups because its retina is similar to the human retina and is homogeneously pigmented and exhibited a high incidence of CNV after photocoagulation.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Laser-Induced Choroidal Neovascularization by Hematoporphyrin Dimethylether-Mediated Photodynamic Therapy in Rats

Yan

Bao

Zhang

et al. 2017

Biological & Pharmaceutical Bulletin

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This study aimed to investigate the effect of hematoporphyrin dimethylether (HDME)-mediated photodynamic therapy for laser-induced choroidal neovascularization (CNV) in adult Brown Norway rats. HDME was administered via tail vein at 14 d after the laser photocoagulation, and the rats received irradiance with a laser light at 570 nm at 15 min after injection. CNV was evaluated by fundus photography, fundus fluorescein angiography, optical coherence tomography, and hematoxylin and eosin staining. We found that CNV was occurred at 7 d after photocoagulation and reaching peak activity at 14 d after photocoagulation. There is a significant reduction in the total area of the fluorescein leakage and the number of strong fluorescein leakage spots on 7 d after HDME-mediated photodynamic therapy (PDT). The results suggest that HDME-mediated PDT inhibits laser-induced CNV in rats, representing a promising therapy for wet age-related macular degeneration.

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“…Using the corrosion cast method, many authors have described the effects of laser photocoagulation in the choroid [30,31] and experimental CNV [5,[32][33][34][35]. To evaluate the incidence of CNV, many techniques, such as FA [1][2][3][4], IA [5] and corrosion cast, have been adopted.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Experimental Choroidal Neovascularization by Irsogladine, an Anti-Gastric Ulcer Agent

Kuroki

Kitaoka²,

Matsuo³

et al. 2003

Ophthalmic Res

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Purpose: To determine whether irsogladine inhibits experimental choroidal neovascularization (CNV) induced by laser photocoagulation in pigmented rats. Methods: Focal laser photocoagulation (argon green 50 mW, 0.04 s, 200 µm) was applied to the retinochoroid of normal Brown Norway rats. Oral administration of irsogladine (5 mg/kg/day or 50 mg/kg/day) was started 1 week before and continued for 2 weeks after laser photocoagulation. Choroidal vascular casts were made 2 weeks after laser photocoagulation and were examined with a scanning electron microscope (SEM). CNV formation was classified according to three grades and evaluated. Results: Laser-induced CNV formation was significantly reduced in rats given 5 mg/kg/day (p < 0.01) or 50 mg/kg/day of irsogladine (p < 0.001). Administration of 50 mg/kg/day of irsogladine was more effective in preventing CNV formation than 5 mg/kg/day (p < 0.001). The development of the vascular bud was especially inhibited by 50 mg/kg/day of irsogladine (p < 0.001). CNVs in rats treated with 50 mg/kg/day of irsogladine looked less well developed than those in controls. There was no significant side effect of irsogladine. Conclusions: Irsogladine inhibits the development of experimental CNV induced by photocoagulation in pigmented rats.

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Choroidal Microvascular Repair after Argon Laser Photocoagulation

Cited by 46 publications

References 8 publications

Natural Course of Experimental Choroidal Neovascularization: Three-Dimensional Study with Corrosion Cast and Scanning Electron Microscope

Natural Course of Experimental Choroidal Neovascularization: Three-Dimensional Study with Corrosion Cast and Scanning Electron Microscope

Inhibition of Laser-Induced Choroidal Neovascularization by Hematoporphyrin Dimethylether-Mediated Photodynamic Therapy in Rats

Inhibition of Experimental Choroidal Neovascularization by Irsogladine, an Anti-Gastric Ulcer Agent

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