Chromatin Immunoprecipitation and DNA Sequencing Identified a LIMS1/ILK Pathway Regulated by LMO1 in Neuroblastoma

Saeki, Norihisa; Saito, Akira; Sugaya, Yuki; Amemiya, Mitsuhiro; Ono, Hiroe; Yanagihara, Kazuyoshi; Sasaki, Hiroki

doi:10.21873/cgp.20074

Cited by 8 publications

(7 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RNA sequencing (RNA-seq) and gene set enrichment analysis (GSEA) indicated that matrisome, extracellular matrix-associated proteins, integrins coding signature genes were enriched in the neuroblastoma cell lines expression high levels of LMO1. Later on, a mechanistic study using chromatin immunoprecipiation and DNA sequencing demonstrated that in neuroblastoma, LMO1 could affect LIMSI ( LIM and senescent cell antigen-like domains 1 ), Ras suppressor protein 1 ( RSU1 ), and relaxin 2 ( RLN2 ) gene, and regulate a carcinogenic LIMSI/integin-linked kinase pathway ( 33 ).…”

Section: Discussionmentioning

confidence: 99%

LMO1 Gene Polymorphisms Reduce Neuroblastoma Risk in Eastern Chinese Children: A Three-Center Case-Control Study

Zhu

Han

et al. 2018

Front. Oncol.

View full text Add to dashboard Cite

Background: Neuroblastoma, a neuroendocrine tumor, stems from the developing sympathetic nervous system. Previous genome-wide association studies (GWASs) have discovered a number of neuroblastoma susceptibility genes in Caucasians including LIM domain only 1 (LMO1).Objective: We conducted a three-center case-control study including 313 cases and 716 controls with the purpose to evaluate the association between five GWAS-identified LMO1 variants (rs110419 A>G, rs4758051 G>A, rs10840002 A>G, rs204938 A>G, and rs2168101 G>T) and neuroblastoma susceptibility in eastern Chinese children.Methods: Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to evaluate the strength of the associations. False positive report possibility (FPRP) analysis was performed to check whether significant results were noteworthy.Results: Significant associations with neuroblastoma risk were found for four (rs110419, rs4758051, rs10840002, and rs2168101) out of the five polymorphisms. Combined analysis demonstrated that carriers of 4–5 protective genotypes had a significantly decreased risk of neuroblastoma in comparison those with 0–3 protective genotypes (adjusted OR = 0.51, 95% CI = 0.39–0.68, P < 0.0001). Haplotype analysis of the five SNPs yield four significant haplotypes associated with neuroblastoma susceptibility.Conclusion: In conclusion, we confirmed LMO1 polymorphisms may reduce neuroblastoma risk in eastern Chinese populations.

show abstract

Section: Discussionmentioning

confidence: 99%

LMO1 Gene Polymorphisms Reduce Neuroblastoma Risk in Eastern Chinese Children: A Three-Center Case-Control Study

Zhu

Han

et al. 2018

Front. Oncol.

View full text Add to dashboard Cite

show abstract

“…A previous study has shown that miR-450b-3p was downregulated in breast cancer and could inhibit HER3 expression through the PI3K/Akt pathway (31). LIM domain only 1 (LMO1), which as a hsa_circ_0021087 host gene, has been reported as an oncogene in multiple tumors, such as lung cancer (32), neuroblastoma (33) and gastric cancer (34). Pathway analysis revealed that it is also involved in several cancer-associated pathways, such as cell cycle, viral carcinogenesis and pathways in cancer.…”

Section: Discussionmentioning

confidence: 99%

A Dual-Circular RNA Signature as a Non-invasive Diagnostic Biomarker for Gastric Cancer

et al. 2020

View full text Add to dashboard Cite

Gastric cancer (GC) is the TOP3 leading cause of human mortality in malignant tumors. Notwithstanding, the association between GC and circRNAs is not clear. The purpose of this research was to determine the association between GC progression and circRNAs. The data of circRNAs was obtained from the Gene Expression Omnibus (GEO) database to identify gene, which differentially expressed circRNAs in GC tissues and paired normal tissues. The expression of circRNAs in cancer tissues and normal tissues were tested, and the target circRNA was verified before and after surgery in the plasma. A circRNA-micro(mi)RNA-mRNA competing endogenous RNAs (ceRNAs) network was established, and GO and KEGG analysis are performed. Five candidate circRNAs were identified through bioinformatics analysis. Hsa_circ_0021087 and hsa_circ_0005051 were both downregulated in GC tissues, cells and plasma by RTq-PCR. Additionally, there was a significant difference in the expression of plasma hsa_circ_0021087 in patients with GC at the preoperative and postoperative stages (P < 0.001). Hsa_circ_0021087 also promoted the proliferation of GC cells in vitro. Next, the circRNA-miRNA-mRNA network of hsa_circ_0021087 was predicted, which may be associated with the development of GC by bioinformatics analysis. In summary, the aforementioned dual-circular RNAs may have important implications on the potential, novel and non-invasive diagnostic method for patients with GC.

show abstract

“…In this study, ChIP-seq analysis demonstrated the direct association of LMO1 protein with these three genes in two neuroblastoma cell lines SK-N-SH and LAN-5. [73] The authors further showed that knocking down LMO1 expression suppressed the expression of the three genes. [73] In subsequent studies conducted by the same research group, it was found that LMO1 indirectly downregulates 18 tumor-suppressive microRNAs in SK-N-SH cells, including hsa-miR-34a-5p and 7 members of the let-7 family, [74] suggesting that downregulating the expression of those miRNAs is one of the mechanisms underlying the oncogenic function of LMO1.…”

Section: The Oncogenic Mechanism Of Lmo1 In Neuroblastomamentioning

confidence: 99%

“…[57] Additional downstream genes of LMO1 have been identified. Saeki et al [73] identified three genes directly regulated by LMO1 at the transcriptional level. These three genes are LIM and senescent cell antigen-like domains 1 (LIMS1), Ras suppressor protein 1 (RSU1), and relaxin 2 (RLN2).…”

Section: The Oncogenic Mechanism Of Lmo1 In Neuroblastomamentioning

confidence: 99%

LIM domain only 1: an oncogenic transcription cofactor contributing to the tumorigenesis of multiple cancer types

Zhao

Zhang

et al. 2021

Chinese Medical Journal

View full text Add to dashboard Cite

The LIM domain only 1 (LMO1) gene belongs to the LMO family of genes that encodes a group of transcriptional cofactors. This group of transcriptional cofactors regulates gene transcription by acting as a key “connector” or “scaffold” in transcription complexes. All LMOs, including LMO1, are important players in the process of tumorigenesis. Unique biological features of LMO1 distinct from other LMO members, such as its tissue-specific expression patterns, interacting proteins, and transcriptional targets, have been increasingly recognized. Studies indicated that LMO1 plays a critical oncogenic role in various types of cancers, including T-cell acute lymphoblastic leukemia, neuroblastoma, gastric cancer, lung cancer, and prostate cancer. The molecular mechanisms underlying such functions of LMO1 have also been investigated, but they are currently far from being fully elucidated. Here, we focus on reviewing the current findings on the role of LMO1 in tumorigenesis, the mechanisms of its oncogenic action, and the mechanisms that drive its aberrant activation in cancers. We also briefly review its roles in the development process and non-cancer diseases. Finally, we discuss the remaining questions and future investigations required for promoting the translation of laboratory findings to clinical applications, including cancer diagnosis and treatment.

show abstract

Chromatin Immunoprecipitation and DNA Sequencing Identified a LIMS1/ILK Pathway Regulated by LMO1 in Neuroblastoma

Abstract: The downstream of LMO1-regulatory cascade includes a tumor-promoting LIMS1/ILK pathway, which has a potential to be a novel therapeutic target.

Cited by 8 publications

References 30 publications

LMO1 Gene Polymorphisms Reduce Neuroblastoma Risk in Eastern Chinese Children: A Three-Center Case-Control Study

LMO1 Gene Polymorphisms Reduce Neuroblastoma Risk in Eastern Chinese Children: A Three-Center Case-Control Study

A Dual-Circular RNA Signature as a Non-invasive Diagnostic Biomarker for Gastric Cancer

LIM domain only 1: an oncogenic transcription cofactor contributing to the tumorigenesis of multiple cancer types

Contact Info

Product

Resources

About