Chromatographic Determination of Aminoacridine Hydrochloride, Lidocaine Hydrochloride and Lidocaine Toxic Impurity in Oral Gel

Abstract

Two sensitive and selective analytical methods were developed for simultaneous determination of aminoacridine hydrochloride and lidocaine hydrochloride in bulk powder and pharmaceutical formulation. Method A was based on HPLC separation of the cited drugs with determination of the toxic lidocaine-related impurity 2,6-dimethylaniline. The separation was achieved using reversed-phase column C18, 250 × 4.6 mm, 5 µm particle size and mobile phase consisting of 0.05 M disodium hydrogen phosphate dihydrate (pH 6.0 ± 0.2 adjusted with phosphoric acid) and acetonitrile (55 : 45, v/v). Quantitation was achieved with UV detection at 240 nm. Linear calibration curve was in the range of 1.00-10.00, 13.20-132.00 and 1.32-13.20 µg mL(-1) for aminoacridine hydrochloride, lidocaine hydrochloride and 2,6-dimethylaniline, respectively. Method B was based on TLC separation of the cited drugs followed by densitometric measurement at 365 nm on the fluorescent mode for aminoacridine hydrochloride and 220 nm on the absorption mode for lidocaine hydrochloride. The separation was carried out using ethyl acetate-methanol-acetic acid (65 : 30 : 5 by volume) as a developing system. The calibration curve was in the range of 25.00-250.00 ng spot(-1) and 0.99-9.90 µg spot(-1) for aminoacridine hydrochloride and lidocaine hydrochloride, respectively. The results obtained were statistically analyzed and compared with those obtained by applying the manufacturer's method.

A simple and fast method for determination of benzocaine and lidocaine in pharmaceutical formulations by capillary electrophoresis with spectrophotometric detection

“…The proposed method demonstrated to be very sensitive, as indicated by the low values (below 1 mg L −1 ) of the LOQ obtained. These LOQs were lower or comparable to those reported in previous works using CE [33,36,37,43], HPLC [12][13][14][15][16][17][18][19][20][21][22], and electrochemical detection [23,[25][26][27]. The intra-day precision was assessed by analyzing in triplicate (in the same day) six different samples of pharmaceutical formulations (three samples containing LD and other three with BZ).…”

“…In addition to HPLC methods of analysis for determination of LD and BZ in pharmaceutical formulations [12][13][14][15][16][17][18][19][20][21][22], analytical methods based on electrochemical [23][24][25][26][27] and spectrophotometric [28][29][30][31] detections have also been reported in the literature. Beyond quantification of LD in pharmaceutical formulations [32][33][34][35], CE has also been applied for the determination of this anesthetic in urine [36][37][38], human plasma [39,40], human serum [41,42], vitreous humor [43], and seized cocaine [6,9].…”

Voltammetric Determination of Lidocaine and Its Toxic Metabolite in Pharmaceutical Formulation and Milk Using Carbon Paste Electrode Modified with C18 Silica

“…Selective and/or simultaneous quantitation of LH and DMA was described using TLC, 4 ISE 29 and HPLC. [30][31][32] As far as to our Knowledge simultaneous determination of LH and DMA has not yet been reported in the literature using voltammetry. Accordingly, the current work was planned to introduce a simple, accurate and sensitive voltammetric method for the assay of LH and DMA simultaneously in their marketed formulations.…”

Novel Aminoacridine Sensors Based on Molecularly Imprinted Hybrid Polymeric Membranes for Static and Hydrodynamic Drug Quality Control Monitoring

“…Adsorptive stripping voltammetry at a static mercury dropping electrode has been used for acridine assessment [8]. Reversed phase HPLC, TLC and GLC have been utilized for separation and quantitation of aminoacridine [9,10] and spectrophotometric and fluorimetric methods for aminoacridine determination [11,12]. Most of these methods suffer from lack of selectivity and sensitivity or involved several time consuming manipulation steps.…”