Chromatographic Estimation of a Novel Triple-Therapy Combination Targeting Helicobacter Pylori Eradication in Different Matrices

Abstract

Aim: Helicobacter pylori infection is a prevalent global bacterial infection that can potentially exaggerate symptoms of other serious infections like SARS-CoV-2 (COVID-19). Methodology: Herein, an efficient, accurate and cost-effective high-performance liquid chromatography-diode array detector method was developed and validated for determination of the novel triple therapy combination of tinidazole (TD), clarithromycin (CLR) and lansoprazole (LAN) in different analytical matrices (pharmaceutical formulation, dissolution media and spiked human plasma). Results: Successful chromatographic separation was achieved using Agilent Microsorb-MV 100–5 CN column (250 × 4.6 mm, 5 μm) and a mobile phase consisted of acetonitrile and 10.0 mM phosphate buffer, pH 7.5 ± 0.1 at flow rate of 1 ml/min via gradient elution. UV-detection was accomplished at 210.0 nm for CLR and 290.0 nm for TD and LAN. Conclusion: The developed method clearly provides a reliable, beneficial and cost-effective tool for quality control, dissolution testing and biological applications of the mentioned drugs.

Ecofriendly high‐performance thin‐layer chromatography‐densitometric method for determination of a new triple pharmaceutical combination targeting Helicobacter pylori eradication along with greenness profile assessment

“…The results were satisfactory, Table 4. The obtained results were statistically compared to those of a reported HPLC method [21]. F ‐and t ‐values were calculated and no significant differences were observed, Supporting information Table S1.…”

“…To the best of our knowledge, no HPTLC method has been reported on this ternary mixture yet. Only one HPLC-DAD method has been described for determination of the cited drugs in pharmaceutical dosage form, however, not following the green chemistry principles [21].…”

Sustainable liquid chromatographic determination and purity assessment of a possible add-on triple-action over-the-counter pharmaceutical combination in COVID-19

“…Chromatography is currently considered as a gold standard technique in pharmaceutical industry, owing to its outstanding advantages such as low operation cost, availability, good sensitivity, small sample size requirements and diversity of applications [29] , [30] , [31] , [32] , [33] . Recently, analysts aimed to apply a competent analytical methodology with improved eco-friendliness and sustainability to preserve our environment from chemical threats [31] , [34] , [35] .…”