Chromosomal studies on ten species of notothenioid fishes (Notothenioidei: Bathydraconidae, Channichthyidae, Nototheniidae)

Caputo, Vincenzo; Cerioni, Paola Nisi; Splendiani, Andrea; Capriglione, Teresa; Odierna, Gaëtano; Olmo, Ettore

doi:10.1159/000071050

Cited by 9 publications

(4 citation statements)

References 50 publications

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“…Application of silver staining has 2 deficiencies: (1) the method reveals nucleolar argyrophilic proteins retained in metaphase chromosomes and, therefore, only the NORs active in the previous interphase (AgNORs) are detected; (2) sometimes, silver binds to chromosome regions not properly corresponding to NORs, as centromeres or particular heterochromatin blocks. The formation of such pseudo-AgNORs, positive in silver staining, has been observed in some fishes [Ozouf-Costaz et al, 1997;Pisano et al, 2000;Caputo et al, 2002;Gromicho et al, 2005] and other ani-mals [Dobigny et al, 2002;Cabrero and Camacho, 2008] and may be due to the sequences that bind the RNA polymerase I transcription factor UBF, integrated at ectopic chromosome sites [Wright et al, 2006].…”

Section: Cytogenetic Methods For Detecting Rdna Sitesmentioning

confidence: 99%

Twenty Years of Physical Mapping of Major Ribosomal RNA Genes across the Teleosts: A Review of Research

Gornung

2013

Cytogenet Genome Res

124

111

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Molecular cytogenetic data on the number and position of 45S ribosomal RNA genes (rDNA; located in nucleolus organizing regions, NORs) detected by FISH in 330 species of 77 families and 22 orders of bony fishes (Teleostei) and, additionally, 11 species of basal ray-finned fishes are compiled and analyzed. The portion of species with single rDNA sites in the sample amounts to 72%. The percentage of species with multiple NORs decreases with increasing numbers of rDNA loci per genome, i.e. scarcely 3% of species carry 4 or more rDNA-bearing chromosome pairs. 43% of all rDNA sites analyzed occur terminally on the short arms of chromosomes or constitute them. In general, terminal rDNA sites account for 87% of all examined cases. Interspecific variation in the location of single rDNA sites among related taxa, polymorphisms of multiple NORs in some groups of teleosts and analytical outcomes on the subject are reviewed.

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Section: Cytogenetic Methods For Detecting Rdna Sitesmentioning

confidence: 99%

Twenty Years of Physical Mapping of Major Ribosomal RNA Genes across the Teleosts: A Review of Research

Gornung

2013

Cytogenet Genome Res

124

111

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“…Such a fusion may explain the presence of the large chromosome pair observed in Artedidraconidae, and may provide a mechanism for the reduction in the diploid number from 48 to 46. Indeed, chromosomal rearrangements involving fusions of one-armed chromosomes to produce karyotypes with reduced numbers of larger elements are common events in the chromosome evolution of vertebrates, including notothenioid fishes (King 1993, Pisano et al 2000, Caputo et al 2002.…”

Section: The Plunderfish Karyotypesmentioning

confidence: 99%

Cytogenetic diversity in the Antarctic plunderfishes (Notothenioidei: Artedidraconidae)

et al. 2010

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Antarctic plunderfishes (Notothenioidei, Artedidraconidae) are important components of the Southern Ocean fish fauna. As a contribution to the Victoria Land Transect Project, we performed a cytogenetic analysis of six species from three of the four artedidraconid genera, Artedidraco glareobarbatus, A. orianae, A. skottsbergi, A. shackletoni, Histiodraco velifer, and Pogonophryne sp. We investigated the species-specific cytogenetic features and highlighted patterns of chromosomal evolutionary change using a molecular phylogeny based on mitochondrial and nuclear genes. Despite a conserved diploid number, some important karyotypic traits account for major differences among artedidraconid species. Specific cytogenetic features, including the chromosomal organization of ribosomal genes and the occurrence of sex chromosomes, are characteristics of A. skottsbergi, making this species distinct among those studied. These chromosomal peculiarities are consistent with the phylogenetic hypothesis resolving A. skottsbergi as the sister lineage of all other Artedidraconidae. A karyological similarity was found between A. glareobarbatus and A. shackletoni consistent with their inferred sister species relationship in the phylogeny. The results indicate that artedidraconids are not conservative in their genomic organization at the chromosomal level and provide new evidence for the degree of biological diversity in this notothenioid group.

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“…In several vertebrate species, including fishes, argentophilic sites not associated with rDNA sites, known as pseudo-NORs, were already described ( Ozouf-Costaz et al 1997 ; Pisano et al 2000 ; Caputo et al 2002 ; Dobigny et al 2002 ; Gromicho et al 2005 ; Cabrero and Camacho 2008 ). Structurally, the BOD regions have similarities with pseudo-NORs that are tandem arrays of a heterologous DNA sequences.…”

Section: Discussionmentioning

confidence: 99%

Differential hypomethylation of the repetitive Tol2/Alu-rich sequences in the genome of Bodianus species (Labriformes, Labridae)

Motta-Neto

Marques

Costa

et al. 2018

CCG

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Representatives of the order Labriformes show karyotypes of extreme conservatism together with others with high chromosomal diversification. However, the cytological characterization of epigenetic modifications remains unknown for the majority of the species. In the family Labridae, the most abundant fishes on tropical reefs, the genomes of the genus Bodianus Bloch, 1790 have been characterized by the occurrence of a peculiar chromosomal region, here denominated BOD. This region is exceptionally decondensed, heterochromatic, argentophilic, GC-neutral and, in contrast to classical secondary constrictions, shows no signals of hybridization with 18S rDNA probes. In order to characterize the BOD region, the methylation pattern, the distribution of Alu and Tol2 retrotransposons and of 18S and 5S rDNA sites, respectively, were analyzed by Fluorescence In Situ Hybridization (FISH) on metaphase chromosomes of two Bodianus species, B. insularis Gomon & Lubbock, 1980 and B. pulchellus (Poey, 1860). Immunolocalization of the 5-methylcytosine revealed hypermethylated chromosomal regions, dispersed along the entire length of the chromosomes of both species, while the BOD regions exhibited a hypomethylated pattern. Hypomethylation of the BOD region is associated with the precise co-location of Tol2 and Alu elements, suggesting their active participation in the regulatory epigenetic process. This evidence underscores a probable differential methylation action during the cell cycle, as well as the role of Tol2/Alu elements in functional processes of fish genomes.

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Chromosomal studies on ten species of notothenioid fishes (Notothenioidei: Bathydraconidae, Channichthyidae, Nototheniidae)

Cited by 9 publications

References 50 publications

Twenty Years of Physical Mapping of Major Ribosomal RNA Genes across the Teleosts: A Review of Research

Twenty Years of Physical Mapping of Major Ribosomal RNA Genes across the Teleosts: A Review of Research

Cytogenetic diversity in the Antarctic plunderfishes (Notothenioidei: Artedidraconidae)

Differential hypomethylation of the repetitive Tol2/Alu-rich sequences in the genome of Bodianus species (Labriformes, Labridae)

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