Chromosome aberrations in spermatogonia and sperm abnormalities in Curacron-treated mice

Nahas, Soheir M. El; Hondt, H. A.; Abdou, Halima E.

doi:10.1016/0165-1218(89)90116-x

Cited by 28 publications

(16 citation statements)

References 21 publications

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“…The reproductive toxicity of profenofos in mammalian germ cells was studied by El Nahas et al (1989). They reported the chromosomal aberrations in spermatogonial cells and sperm abnormalities in mice after profenofos treatment, and showed damaging effect on spermatogonial cells via significant increase in structural chromosomal aberrations and on sperm morphology via significant increase in sperm abnormalities and significant decrease in sperm count and motility.…”

Section: Introductionmentioning

confidence: 99%

Testicular toxicity of profenofos in matured male rats

et al. 2007

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To investigate the effect of the phosphorothoate insecticide profenofos on male specific gene expression on rat testis, 16-week-old Wistar rats were orally administered at dose of 17.8 mg/kg twice weekly for 65 days. Gene expression in the testes was monitored by DNA microarray analysis and real time RT-PCR which revealed that genes related to steroidogenesis including cytochrome P450 17A1 (CYP17A1), steroidogenic acute regulatory protein (StAR) and CYP11A1 were significantly increased. Besides the testes were histopathologicaly examined which revealed testicular destruction and degeneration represented by a layer of columner epithelium, oedematous changes surrounding the siminiferous tubules besides vacuolated spermatogonial calls and more elongated Leydig cells. These data suggest that profenofos considered as one of the male reproductive toxicants. Furthermore, we propose that the above 3 steroidogenic-related genes and the gene of acrosomal reaction as potential biomarkers of testicular toxicity.

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Section: Introductionmentioning

confidence: 99%

Testicular toxicity of profenofos in matured male rats

et al. 2007

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“…23 Many other organophosphorus insecticides have been reported to cause chromosomal aberrations in mammalian cells. 15,18,[24][25][26][27][28] With respect to the other insecticides, buprofezin and Super Royal, the present results indicate that in addition to their low mammalian toxicity they had a weak clastogenic effect in somatic…”

Section: Resultsmentioning

confidence: 92%

Genotoxicity evaluation of buprofezin, petroleum oil and profenofos in somatic and germ cells of male mice

Fahmy

Abdalla

1998

J. Appl. Toxicol.

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The two pest control agents, buprofezin and petroleum oil (Super Royal), were tested to evaluate their potential mutagenicity, in comparison with the organophosphorus insecticide profenofos. Chromosomal aberration analysis was used in both somatic and germ cells of male mice. Single oral treatment at three different dose levels (1/16, 1/8 and 1/4 LD 50) for each insecticide induced an increase in the percentage of chromosomal aberrations in bone‐marrow cells 24 h post‐treatment, indicating a dose‐dependent relationship. The percentage of chromosomal aberrations reached 23 ± 0.73, 10.5 ± 0.64 and 15 ± 1.4 after treatment with the highest tested dose of profenofos, buprofezin and Super Royal, respectively. Such percentages did not exceed the corresponding value of the positive control, mitomycin C (29.2 ± 0.69). The percentage of chromosomal aberrations induced by the different doses of profenofos was still highly significant even after excluding gaps. The same trend of results was noticed only at the highest tested dose of buprofezin and Super Royal. With respect to germ cells, profenofos is also a potent inducer of chromosomal aberrations in 1ry spermatocytes, giving percentages of 14 ± 1.3 and 19 ± 1.6 at the two higher doses of 4.25 and 8.5 mg kg−1 body wt., respectively. Buprofezin and Super Royal had no significant effect on mouse spermatocytes at the tested concentrations. The various types of induced aberrations were examined and recorded in both somatic and germ cells. In conclusion, the present investigation indicates that the two pest control agents buprofezin and Super Royal are relatively much safer compounds than the conventional organophosphorus insecticides. © 1998 John Wiley & Sons, Ltd.

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“…Reddy and Rao [23], yine bu insektisite maruz kalan alabalık solucanlarında (Eisenia foetida) vücutta kopmalar, lezyonlar, hücre bezeleri ve kas parçalanmaları şeklinde çeşitli histolojik ve morfolojik değişiklikler gözlerken İsmail et al [4]'da sazan balıklarında (Cyprinus carpio) denge, hareket ve davranış bozukluklarının meydana geldiğini rapor etmiştir. Farelerde anormal sperm oluşumuna, sperm sayısının ve canlılığının azalmasına [24,25], 16 haftalık wistar sıçanlarında ise seminifer tüplerinde ve lyding hücrelerinde yapısal bozulmalara yol açtığı da rapor edilmiştir [1]. Abdullah et al [26]'a göre, profenofos Avustralya tatlı su karideslerinde (Paratya australiensis) asetilkolinesteraz enzimini inhibe ederek nötoksik etkiye de sebep olmaktadır.…”

Section: Discussionunclassified

“…Bu konuya yönelik daha önce yapılmış çeşitli çalışmalar bulunmaktadır. Bu çalışmalardan bir kaçında; profenofosun farelerin spermatogoniumlarında, sperm ve kemik iliği hücrelerinde kromozomal bozukluklara yol açtığı [24,27], sıçanların polikromatik eritrositlerde mikronükleus frekansını artırdığı [5], insan periferal lenfosit hücrelerinde ise kromozomal kırıklara, gaplara ve DNA hasarlarına neden olduğu [6] görülmüştür. Bu araştırmacılara göre, profenofosun toksik ve genotoksik etkileri onun oksidatif stresi artırıcı özelliklerinden kaynaklanmaktadır.…”

Section: Discussionunclassified

Farklı Test Sistemleri ile Somatik Hücrelerde Profenofos Genotoksisitesine Karşı Kuşburnu (Rosa canina L.) Ekstrelerinin Doğal Bir Antigenotoksik Ajan Olarak Kullanılması

Kasimoğlu¹,

Uysal²

2016

CFD

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Özet. Bu çalışmada, profenofos insektisitinin genotoksik etkileri, Drosophila melanogaster'de somatik mutasyon ve rekombinasyon testi (SMART) ve insan periferal lenfosit hücrelerinde mikronükleus (MN) testi ile araştırılmıştır. Bu maddenin olası genotoksik etkilerinin giderilebilmesi için de kuşburnu (Rosa canina) bitkisine ait su ve etanol ekstreleri kullanılmıştır.SMART için D.melanogaster'in kanat preparatları incelendiği zaman, profenofosun artan konsantrasyonuna (0,025, 0,05, 0,075 ve 0,1ppm) bağlı olarak normal kanat fenotipinde mutasyon frekansının arttığı özellikle en yüksek uygulama grubunda (0,1 ppm) bu artışın pozitif etkili (+) olduğu gözlenmiştir (P<0,05). Serrat kanat fenotipinde ise mutasyon frekansındaki artış tüm uygulama gruplarında önemsiz etkili (i) bulunmuştur (P>0,05). Kuşburnu bitkisinin su (RCsu) ve etanol (RCeta) ekstreleri, profenofosun en yüksek konsantrasyonu (0,1 ppm) ile birlikte uygulandığı zaman (profenofos+ RCsu/RCeta) mutasyon frekansındaki artışın azaldığı ve bunun da istatistiksel olarak önemli olduğu gözlenmiştir (P<0,05). The Usage of Rosa canina L. Extracts As a Natural Anti-Genotoxic Agents Against the Genotoxicity of Profenofos on Somatic Cells by Different Test SystemsAbstract. In this study, the genotoxic effects of profenofos insecticide were investigated with the somatic mutation and recombination test (SMART) in Drosophila melanogaster and the micronucleus test (MN) in human peripheral lymphocytes. The water and ethanol extracts of rosehip plants (Rosa canina) were used to remove possible genotoxic effects of this substance.When the wing preparates of D.melanogaster were examined for the SMART, it was observed that mutation frequency in normal wing phenotype increased depending on doses of profenofos (0,025, 0,05, 0,075 ve 0,1ppm) and this increase was positive effect (+) in the highest application group (0,1 ppm), especially. The increase in mutation frequency of the serrate wings phenotype was found insignificant (i) in the all application groups (P>0,05). When the water (RCwtr) and ethanol (RCeth) extracts of the rosehip plants were applied together with the highest application concentration of profenofos (profenofos+ RCwtr/RCeth), it was observed that the increase in the mutation frequency decreased and this decrease was statistically significant (P<0,05).In this study, the mutation frequency increased and nuclear division index (NBI) decreased in the human lymphocyte cells with in vitro profenofos application in the different concentration were showed (P<0,05). But as the MN frequencies decreased, NBI values approached to control group with profenofos+RCwtr and RCeth applications (P<0,05).

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Chromosome aberrations in spermatogonia and sperm abnormalities in Curacron-treated mice

Cited by 28 publications

References 21 publications

Testicular toxicity of profenofos in matured male rats

Testicular toxicity of profenofos in matured male rats

Genotoxicity evaluation of buprofezin, petroleum oil and profenofos in somatic and germ cells of male mice

Farklı Test Sistemleri ile Somatik Hücrelerde Profenofos Genotoksisitesine Karşı Kuşburnu (Rosa canina L.) Ekstrelerinin Doğal Bir Antigenotoksik Ajan Olarak Kullanılması

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