Chromosome duplication in Lolium multiflorum Lam.

Pereira, Rafaela Corrêa; Ferreira, Marco Túlio Mendes; Davide, Lisete Chamma; Pasqual, Moacir; Mittelmann, Andréa; Techio, Vânia Helena

doi:10.1590/1984-70332014v14n4n39

Cited by 21 publications

(27 citation statements)

References 18 publications

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“…Poliplodization is an important strategy used in forage breeding programs, because it may increase the expression of agronomic traits of interest, restore fertility of intra-and interspecific hybrids, and, in some cases, permit the exploration of genetic variability in apomictic species by duplicating sexual plants and equaling the ploidy to allow the accomplishment of crossings and obtaining fertile offspring (PEREIRA et al, 2001;NAIR, 2004;BARBOSA et al, 2007;CAMPOS et al, 2009;ISHIGAKI et al, 2009;SIMIONI & VALLE, 2009;PEREIRA et al, 2014;TIMBÓ et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In Brazil, polyploid genotypes have already been obtained in several forage species and hybrids such as: Brachiaria brizantha, Brachiaria decumbens, Brachiaria ruziziensis and hybrids between B. decumbens, B. brizantha and B. ruziziensis (PINHEIRO et al, 2000;SIMIONI & VALLE 2009;TIMBÓ et al, 2014), triploid hybrids of Pennisetum glaucum x Pennisetum purpureum (BARBOSA et al, 2007;CAMPOS et al, 2009) and Lolium multiflorum (PEREIRA et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Stability in chromosome number and DNA content in synthetic tetraploids of Lolium multiflorum after two generations of selection

et al. 2017

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Chromosome doubling of Italian ryegrass genotypes ( Lolium multiflorum Lam.) adapted to the brazilian edaphoclimatic conditions is an important strategy used by breeders and aims to obtain more vigorous genotypes with better forage quality and disease resistance. The effectiveness of chromosome doubling can be measured by genetic stability and fertility rates of plants over generations. However, a common problem in the polyploidization process is the regeneration of mixoploid plants that have impaired fertility and genetic stability. The objective of this study was to verify if progenies of recently tetraploidized plants remain stable regarding DNA content and chromosome number, over two generations. Progenies of L. multiflorum plants artificially tetraploidized with colchicine treatment were evaluated. Chromosome counting and estimates of the DNA content were used to evaluate the genetic stability. The percentage of tetraploid plants (4X) increased over generations (18%, 34% and 91% in cycle 0, 1 and 2, respectively). All progenies identified as tetraploid by flow citometry showed variation in chromosome number (mixoploidy), but produced viable seeds. Results showed that stabilization in chromosome number and DNA content in tetraploidized plant progenies requires time and that the success of this procedure depends on a continuous and accurate screening and selection.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Stability in chromosome number and DNA content in synthetic tetraploids of Lolium multiflorum after two generations of selection

et al. 2017

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“…The percentage of polyploids obtained was 20%. The polyploiding effect of colchicine was obtained with 24 h exposure (Pereira et al 2014). …”

Section: Resultsmentioning

confidence: 99%

Chromosome doubling in Paspalum notatum var. saure (cultivar Pensacola)

Weiler

Krycki

Guerra

et al. 2015

Crop Breed. Appl. Biotechnol.

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“…LOL 161) and L. perenne ('Ellet'), both 2n = 2x = 14 (Pereira et al, 2014;Rocha et al, 2015), in addition to two F. arundinacea cultivars ('Rizomat' and 'Quantum II') 2n = 4x = 42. The Lolium species came from Banco Ativo de Germoplasma de Plantas Forrageiras (Active Germplasm Bank) and Embrapa Recursos Genéticos e Biotecnologia.…”

Section: Methodsmentioning

confidence: 99%

Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca

et al. 2017

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ABSTRACT. Analyses carried out with fluorescence in situ hybridization (FISH) in C-metaphases of the Lolium-Festuca complex have shown the occurrence of spontaneous fragile sites (FSs) in 45S rDNA regions. FSs are expressed as gaps but they do not result in breaks or chromosomal fragments in these species. These gaps have high DNA condensation observed as thin chromatin fibers that connect the apparent segments of the fragile chromosome, allowing for genomic stability. Assessing the behavior of these regions in the cell cycle of Lolium and Festuca species may lead to a better understanding of the dynamics that preserve stability during cell division. Furthermore, it is interesting to track the dynamics of chromosomes bearing 45S rDNA sites in the cell cycle as well as to observe the expression of FSs with no effect of the mitotic block. We observed variation in both the number and size of 45S FISH signals from the S/G2 phases of interphase and from prophase to anaphase where gaps in 45S rDNA sites also were observed. The change in the degree of condensation of the 45S site begins in the S/G2 phase and appears to be related to the transcriptional demand. Taking into account that the number of 45S rDNA sites tends to be reestablished when cells reach telophase, we suggest that the chromatin fiber goes back to the normal condensation level to the anaphase (after segregation), allowing for the approximation of chromosome segments and ensuring dynamics that favor the genomic stability of these species.

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Chromosome duplication in Lolium multiflorum Lam.

Abstract: -Artificial chromosome duplication of diploid genotypes of

Cited by 21 publications

References 18 publications

Stability in chromosome number and DNA content in synthetic tetraploids of Lolium multiflorum after two generations of selection

Stability in chromosome number and DNA content in synthetic tetraploids of Lolium multiflorum after two generations of selection

Chromosome doubling in Paspalum notatum var. saure (cultivar Pensacola)

Dynamics of 45S rDNA sites in the cell cycle: fragile sites and chromosomal stability in Lolium and Festuca

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