Chromosome mis-segregation and cytokinesis failure in trisomic human cells

Abstract

Cancer cells display aneuploid karyotypes and typically mis-segregate chromosomes at high rates, a phenotype referred to as chromosomal instability (CIN). To test the effects of aneuploidy on chromosome segregation and other mitotic phenotypes we used the colorectal cancer cell line DLD1 (2n = 46) and two variants with trisomy 7 or 13 (DLD1+7 and DLD1+13), as well as euploid and trisomy 13 amniocytes (AF and AF+13). We found that trisomic cells displayed higher rates of chromosome mis-segregation compared to their euploid counterparts. Furthermore, cells with trisomy 13 displayed a distinctive cytokinesis failure phenotype. We showed that up-regulation of SPG20 expression, brought about by trisomy 13 in DLD1+13 and AF+13 cells, is sufficient for the cytokinesis failure phenotype. Overall, our study shows that aneuploidy can induce chromosome mis-segregation. Moreover, we identified a trisomy 13-specific mitotic phenotype that is driven by up-regulation of a gene encoded on the aneuploid chromosome.DOI: http://dx.doi.org/10.7554/eLife.05068.001

Aneuploidy: Cancer strength or vulnerability?

“…77 Moreover, cultured hPSCs tend to acquire trisomy of chromosome 12, which is the most common chromosomal aberration in germ cell tumors, 43 and trisomy of chromosome 13, which confers a distinctive cytokinesis failure phenotype to colon cancer cells. 66 Although the spectrum and degree of aneuploidy differ among tumors, many human cancers share recurrent aneuploidies. 78 It is currently believed that tumor-specific aneuploidies coexist with recurrent aneuploidies across tumors.…”

“…In particular, chromosome segregation errors (specifically chromatin bridges, which typically arise as a result of DNA damage) can lead to the accumulation of postmitotic DNA damage due to the “trapping” of chromatin bridges in the cleavage furrow . Moreover, trisomic colon cancer cells display a higher rate of chromosome missegregation than that of euploid ones, and the capacity of accurate chromosome segregation decreases in a discontinuous way compared to chromosome number changes . Indeed, yeast cells with a ploidy between 1.5 and 2 are more susceptible to chromosome missegregation than those with a near haploid karyotype .…”

“…Reduced BUB1 expression favors the development of thymic lymphoma and colon cancer in tp53 +/─ and apc Min/+ mice, respectively, but suppresses prostatic intraepithelial neoplasia in pten +/─ mice and does not alter the frequency of pituitary tumors and sarcoma formation in the rb +/─ model . Moreover, cultured hPSCs tend to acquire trisomy of chromosome 12, which is the most common chromosomal aberration in germ cell tumors, and trisomy of chromosome 13, which confers a distinctive cytokinesis failure phenotype to colon cancer cells …”

Recurrent patterns of DNA copy number alterations in tumors reflect metabolic selection pressures

“…Moreover, addition of a single chromosome in MEF cells induces a stress response that impairs proliferation and immortalization (Williams et al, 2008). However, numerical aneuploidy can lead to chromosomal instability (Nicholson et al, 2015) which results in subchromosomal gains and losses as observed in human tumors, in mouse models of cancer, and in immortalized MEF cells. Thus, aneuploidy can cause an initial fitness loss due to the costs of dealing with non-optimized chromosome numbers, gene copy numbers, and resulting proteomic imbalances.…”

The two sides of chromosomal instability: drivers and brakes in cancer

“…30,32,69 There are several key indicators of CIN, including lagging chromosomes, chromosome bridges, micronuclei, aneuploidy, and polyploidy. [70][71][72][73][74][75][76][77][78][79][80][81][82][83][84][85][86] As will be discussed below, aberrant spindle assembly checkpoint (SAC) activity, impaired sister chromatid segregation, aberrant centrosome number, and microtubule-kinetochore attachment error could lead to chromosome missegregation. This could in turn increase the formation of lagging chromosomes, which are chromosome that moves to the poles of the cell during cell division slower than other chromosomes, and chromosome bridges, which are structures formed when part of sister chromatids intertwines and fails to completely segregate.…”

Compromised Mitotic Fidelity in Human Pluripotent Stem Cells

“…When cells produce extra amounts of proteins, the fine balance in these tightly regulated processes can be disrupted, resulting in metabolic and replication stress. Furthermore, having extra copies of chromosomes can in itself affect mitosis, resulting in chromosomal instability and in further mitotic stress [85,90]. Also, specific aneuploidies differentially impair the post-implantation developmental potential of human embryos, resulting in diverse developmental fates [91].…”

The fate of extra centrosomes in newly formed tetraploid cells: should I stay, or should I go?

“…The linear fit and regression value (R 2 ) are also shown in the graph. To calculate the fraction of ana-telophase cells with lagging chromosomes for each cell line, data from the Pellman ( Ganem et al, 2009 ), Compton ( Thompson and Compton, 2008 ), and Cimini ( Silkworth et al, 2009 ; Nicholson and Cimini, 2013 ; Nicholson et al, 2015 ) labs were averaged (if applicable). Centrosome amplification data for the cell lines are from Marteil et al ( Marteil et al, 2018 ) and Baudoin et al ( Baudoin et al, 2020 ).…”

Chromosome evolution screens recapitulate tissue-specific tumor aneuploidy patterns

“…2b, white triangles). Most balanced diploid control cultures also gained CNAs over extended time , and structural variation (SV) [19][20][21] . Whole-genome duplication (WGD) occurs often during tumorigenesis and is associated with intra-tumoral heterogeneity [22][23][24][25][26] , therapeutic resistance and poorer outcomes [27][28][29] .…”

Modeling specific aneuploidies: from karyotype manipulations to biological insights

“…These strains have been used to uncover generic cellular responses to aneuploidy (Torres et al 2007 ; Pavelka et al 2010 ; Beach et al 2017 ; Ravichandran et al 2018 ). Subsequent analyses of human and mouse cell lines carrying specific trisomies or monosomies have revealed very similar effects on cell physiology as seen in yeast, including impaired cellular fitness and proliferative potential due to the proteotoxic, metabolic and replication stresses associated with chromosomal gains, or impaired ribosomal biogenesis linked to chromosomal losses (Williams et al 2008 ; Stingele et al 2012 ; Nicholson et al 2015 ; Meena et al 2015 ; Ohashi et al 2015 ; Santaguida et al 2015 ; Passerini et al 2016 ; Passerini et al 2016 ; Chunduri et al 2021 ).…”

“…More recent studies focused on the generic and specific effects of chromosomal gains on cell physiology. Analyses of transformed and non-transformed human cell lines with a single trisomic chromosome (Supplemental table 1 ) revealed that a chromosome gain often leads to increased CIN, replication stress, as well as global transcriptomic and proteomic changes (Phillips et al 2001 ; Phillips et al 2001 ; Nawata et al 2011 ; Stingele et al 2012 ; Dürrbaum et al 2014 ; Nicholson et al 2015 ; Passerini et al 2016 ). Furthermore, in line with earlier observations (Yoshida et al 2000 ; Meaburn et al 2005 ; Kugoh et al 2016 ), these recent studies also showed that nearly all single chromosomal gains negatively impact cellular transformation and metastasis formation.…”

“…Meanwhile, trisomy of chr7 and chr13 conferred a growth advantage to colorectal cancer DLD-1 cells when cultured under challenging conditions such as hypoxia or low serum, although these aneuploid cells proliferated slower than their euploid counterparts in standard culture conditions (Rutledge et al 2016 ). Interestingly, trisomy 13, but not trisomy 7, was found to cause cytokinesis failure and elevated CIN in human colorectal cancer DLD-1 cells due to SPG20 overexpression (Nicholson et al 2015 ). Despite these significant findings, their clinical relevance and the molecular mechanisms by which these aneuploidies potentially drive cancer progression and metastasis require further investigation.…”

The emerging links between chromosomal instability (CIN), metastasis, inflammation and tumour immunity

“…Experiments found that cells with a single chromosome addition often displayed more subsequent chromosome gains or losses [10]. Moreover, these cells displayed causes and/or characteristics of CIN, such as (ultrafine) anaphase bridges [10], micronuclei [11], chromosome mis-segregation and cytokinesis failure [12]. Aneuploidy in itself therefore also seems to be a possible ‘gateway’ to increasingly elevated CIN.…”

A motor-based approach to induce chromosome-specific mis-segregations in human cells

“…Various approaches have been developed to model specific whole-chromosome and arm-level aneuploidies, including microcell-mediated chromosome transfer 20 , Cre-lox recombination of homologs to generate acentric and dicentric chromosomes 21 , CRISPR/Cas9-mediated arm-level and wholechromosome deletion 7,22 , and centromere inactivation of chromosome Y by inducible degradation of CENP-A 23 . Although these methods have generated valuable cell lines with particular chromosomal gains and losses 24,25 , all of these approaches rely on clonal expansion, and therefore cells may have evolved during cell culture following the initial karyotype change. Complementary to these targeted studies, others have assessed the short-term consequences of random karyotype changes in human cells predominantly after mitotic checkpoint inhibition [26][27][28] .…”

Aneuploid Cell Survival Relies upon Sphingolipid Homeostasis

“…Aneuploid mammalian and yeast cells exhibit proliferation defects (3–5) and genomic instability (6–9). In addition, cells harboring whole chromosome gains and losses display metabolic alterations (5,10) and experience proteotoxic stress, which is caused by aneuploidy-induced changes in protein abundance that place an increased demand on the cell’s protein folding and degradation machineries (3,4,11–13).…”