Chromosomes as Tools for Discovering Biodiversity – The Case of Erythrinidae Fish Family

Cioffi, Marcelo de Bello; Molina, Wagner Franco; Artoni, Roberto Ferreira; Bertollo, Luiz Antônio Carlos

doi:10.5772/35890

Cited by 17 publications

(10 citation statements)

References 46 publications

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“…Interestingly, cytogenetic evidence largely supports the geographic restriction of the mitochondrial lineage (ABZ3047 and Group 1) from Guiana Shield suggested by DNA Barcoding data. In particular, the karyomorph F from populations of Suriname is shared only with populations of Amazon and northern Atlantic drainages rivers of Brazil [ 100 ].…”

Section: Discussionmentioning

confidence: 99%

A continental-wide molecular approach unraveling mtDNA diversity and geographic distribution of the Neotropical genus Hoplias

et al. 2018

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With an estimate of around 9,000 species, the Neotropical region hosts the greatest diversity of freshwater fishes of the world. Genetic surveys have the potential to unravel isolated and unique lineages and may result in the identification of undescribed species, accelerating the cataloguing of extant biodiversity. In this paper, molecular diversity within the valuable and widespread Neotropical genus Hoplias was assessed by means of DNA Barcoding. The geographic coverage spanned 40 degrees of latitude from French Guiana to Argentina. Our analyses revealed 22 mitochondrial lineages fully supported by means of Barcode Index Number, Automatic Barcode Gap Discovery and phylogenetic analyses. This mtDNA survey revealed the existence of 15 fully supported mitochondrial lineages within the once considered to be the continentally distributed H. malabaricus. Only four of them are currently described as valid species however, leaving 11 mitochondrial lineages currently “masked” within this species complex. Mean genetic divergence was 13.1%. Barcoding gap analysis discriminated 20 out of the 22 lineages tested. Phylogenetic analyses showed that all taxonomically recognized species form monophyletic groups. Hoplias malabaricus sensu stricto clustered within a large clade, excluding the representatives of the La Plata River Basin. In the H. lacerdae group, all species but H. curupira showed a cohesive match between taxonomic and molecular identification. Two different genetic lineages were recovered for H. aimara. Given the unexpected hidden mitochondrial diversity within H. malabaricus, the COI sequence composition of specimens from Suriname (the type locality), identified as H. malabaricus sensu stricto, is of major importance.

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Section: Discussionmentioning

confidence: 99%

A continental-wide molecular approach unraveling mtDNA diversity and geographic distribution of the Neotropical genus Hoplias

et al. 2018

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“…This condition is even more remarkable for freshwater fishes since their dispersal can be limited and often confined to isolated hydrographic systems [ 5 ]. Such a situation is particularly true for the Erythrinidae family, in which intra-specific chromosomal differentiation has been highlighted for several of its representatives [ 6 , 7 ]. A significant amount of cytogenetic data highlight the diversity displayed by populations of its three genera, including karyotypes with different diploid numbers (2n), chromosomal morphology, and sex chromosome systems, some of which are already well differentiated while others display an early stage of differentiation [ 6 , 7 , 8 , 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

High Genetic Diversity despite Conserved Karyotype Organization in the Giant Trahiras from Genus Hoplias (Characiformes, Erythrinidae)

Sassi

Perez

Oliveira

et al. 2021

Genes

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In the fish genus Hoplias, two major general groups can be found, one of which is formed by the “common trahiras” (Hoplias malabaricus group) and the other by the “giant trahiras” (Hoplias lacerdae group, in addition to Hoplias aimara), which usually comprises specimens of larger body size. Previous investigations from the giant trahiras group recovered 2n = 50 meta/submetacentric chromosomes and no sex chromosome differentiation, indicating a probable conservative pattern for their karyotype organization. Here, we conducted comparative cytogenetic studies in six giant trahiras species, two of them for the first time. We employed standard and advanced molecular cytogenetics procedures, including comparative genomic hybridization (CGH), as well as genomic assessments of diversity levels and phylogenetic relationships among them. The results strongly suggest that the giant trahiras have a particular and differentiated evolutionary pathway inside the Hoplias genus. While these species share the same 2n and karyotypes, their congeneric species of the H. malabaricus group show a notable chromosomal diversity in number, morphology, and sex chromosome systems. However, at the same time, significant changes were characterized at their inner chromosomal level, as well as in their genetic diversity, highlighting their current relationships resulting from different evolutionary histories.

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“…In this respect, chromosomal mapping of specific sequences using fluorescence in situ hybridization (FISH), which enhances the detection of homeologous regions in the genomes of different species [reviewed in Cioffi and Bertollo, 2012], is still restricted in Gobiidae [Mandrioli et al, 2001;Ocalewicz and Sapota, 2011] and has only recently been applied in population analyses [Li-ma-Filho et al, 2012]. In some fish groups, repetitive sequences have shown abrupt evolutionary modifications, with marked differentiation even among closely related species [Mantovani et al, 2004;Abel et al, 2006], whereas in other groups they do not seem to play an obvious role in the diversification of the karyotype [Molina, 2007;Motta-Neto et al, 2011].…”

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Karyotype Divergence and Spreading of 5S rDNA Sequences between Genomes of Two Species: Darter and Emerald Gobies (<b><i>Ctenogobius</i></b>, Gobiidae)

Lima-Filho

Bertollo

Cioffi

et al. 2014

Cytogenet Genome Res

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Karyotype analyses of the cryptobenthic marine species Ctenogobius boleosoma and C. smaragdus were performed by means of classical and molecular cytogenetics, including physical mapping of the multigene 18S and 5S rDNA families. C. boleosoma has 2n = 44 chromosomes (2 submetacentrics + 42 acrocentrics; FN = 46) with a single chromosome pair each carrying 18S and 5S ribosomal sites; whereas C. smaragdus has 2n = 48 chromosomes (2 submetacentrics + 46 acrocentrics; FN = 50), also with a single pair bearing 18S rDNA, but an extensive increase in the number of GC-rich 5S rDNA sites in 21 chromosome pairs. The highly divergent karyotypes among Ctenogobius species contrast with observations in several other marine fish groups, demonstrating an accelerated rate of chromosomal evolution mediated by both chromosomal rearrangements and the extensive dispersion of 5S rDNA sequences in the genome.

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Chromosomes as Tools for Discovering Biodiversity – The Case of Erythrinidae Fish Family

Cited by 17 publications

References 46 publications

A continental-wide molecular approach unraveling mtDNA diversity and geographic distribution of the Neotropical genus Hoplias

A continental-wide molecular approach unraveling mtDNA diversity and geographic distribution of the Neotropical genus Hoplias

High Genetic Diversity despite Conserved Karyotype Organization in the Giant Trahiras from Genus Hoplias (Characiformes, Erythrinidae)

Karyotype Divergence and Spreading of 5S rDNA Sequences between Genomes of Two Species: Darter and Emerald Gobies (<b><i>Ctenogobius</i></b>, Gobiidae)

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