Chronic caffeine intake affects lysozyme activity and immune cells in mice

Abstract

The aim of this study was to examine the effect of caffeine on the activity of lysozyme and some immune parameters of mice. The mice were divided into five groups. Group 1, the control group, was given water. The other four groups were administered various concentrations of caffeine by oral intubation (group 2, 2 mg x kg(-1); group 3, 20 mg x kg(-1); group 4, 40 mg x kg(-1); group 5, 200 mg x kg(-1)). It was found that the activity of lysozyme in the serum depended on the caffeine dose. Compared with the control (group 1), lysozyme activity was 1.4-times higher in group 2, 1.6-times higher in the group 3, and 1.8-times higher in groups 4 and 5 (P < 0.05). In group 3 a significant increase in spleen weight was detected and the spleen index was 2.1-times (P < 0.05) higher compared with control. In group 3 the number of monocytes and neutrophils was 2.5-times higher (P < 0.05) compared with control. In group 5 the caffeine increased the number of neutrophils 2.7-times and increased the number of eosinophils 4.6-times (P < 0.05) compared with control. Our study revealed that caffeine played an important role in the development of protective immune response.

Excessive caffeine intake increases bone resorption associated with periapical periodontitis in rats

“…However, Muqaku et al, (2016) reported pro‐inflammatory effects of caffeine since it has an inhibitory action on immune modulation by regulatory T cells (Tregs), a class of specialized T lymphocytes that controls the immune response, acting on the immune tolerance mechanism to inhibit both proliferation and activity of T helper and cytotoxic T cells (Vignali et al, 2008). An exacerbated inflammatory infiltrate composed mainly of polymorphonuclear cells (neutrophils, eosinophils and basophils) was noted in the CAF group, corroborating previous investigations that reported an increase of 2.5 – 2.7 times in the quantity of these cells in blood when a high dose of caffeine was administrated (Ramanaviciene et al, 2004).…”

Biocompatibility of polypyrrole particles: an in-vivo study in mice

“…29, 08409 Vilnius, Lithuania et al 2003). It is therefore important to test the influence of this polymer on the immune system of model animals, on immune-related haematological parameters and peritoneum cells (Ramanaviciene et al 2004).…”

Surface plasmon resonance label‐free monitoring of antibody antigen interactions in real time

“…1 Various biologically active materials ranging from small organics [1] to huge multiprotein complexes [2] play an incredible role in the physiological functions of living species. Fast and efficient analysis of such compounds is required in molecular biology and biochemistry [3,4].…”