Chronic exposure to pentavalent arsenic of larval leopard frogs (Rana pipiens): bioaccumulation and reduced swimming performance

Abstract

Arsenic (As) is mainly released to the environment from anthropogenic sources, with inorganic pentavalent As (As [V]) predominant in surface water. In this study, Rana pipiens were exposed to As (V) in water at environmentally relevant concentrations (control, 10, 20, 150, 500, and 1,000 microg l(-1)) in a static-renewal system from post-hatch stage through metamorphosis for 113 days. There was no significant effect of As exposure on tadpole survival, growth, and percent metamorphosis. Maximum swimming speed was significantly slower in the As-treated groups compared with the control. During the period of tail resorption (Gosner stage 42-46), no significant differences in age at metamorphosis, survival, length of tail resorption period, snout-vent length of metamorphs, and sex ratio were found among treatments. Whole body As concentrations ranged from <0.6 to 5.31 mg kg(-1) dry mass, and were significantly higher in the 150, 500, and 1,000 microg l(-1) treatments than the control. Based on our data, larval tissue concentrations of As close to that reported in previous field studies were not associated with any significant effects except decreased tadpole swimming speed.

The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics

“…Although some arsenic is released into the environment from natural geogenic sources, e.g., volcanic activity (World Health Organization, 1981;Sanders et al, 1994), the major contributors to arsenic contamination of environment are anthropogenic activities, such as mining, smelting, and the production of fertilizers and pesticides (Agency for Toxic Substances and Disease Registry, 2007;Irving et al, 2008;Chen et al, 2009). Arsenic is toxic to most organisms and in mammals it causes diverse effects, including cancer and cardiovascular disease (Hughs, 2002).…”

Sodium arsenite delays the differentiation of C2C12 mouse myoblast cells and alters methylation patterns on the transcription factor myogenin

“…Alterations in muscle regulatory factors during zebrafish somitogenesis resulted in impaired development of fast skeletal muscle (Tilton and Tanguay, 2008). Additionally, arsenic exposure to frogs prior to metamorphosis resulted in reductions in swimming ability (Chen et al , 2009). …”

Microevolution due to pollution in amphibians: A review on the genetic erosion hypothesis

“…For example, acidification is a serious problem in habitats suitable for amphibians (Hangartner, 2012;R€ as€ anen et al, 2005;Rowe and Freda, 2000;Cook, 1983;Dunson and Connell, 1982;Fioramonti et al, 1997;Pierce and Sikand, 1985;Pough, 1976;Blem and Blem, 1989;Clark, 1986;Dale et al, 1985;Freda, 1986;Pierce, 1985;Rowe et al, 1992) as well as increased UV radiation (O'Brien and Allentoft, 2010;Blaustein and Bancroft, 2007;Blaustein and Kiesecker, 2002;Alton et al, 2010;Blaustein et al, 2003Blaustein et al, , 2008Belden and Blaustein, 2002;Garcia et al, 2004;Langhelle et al, 1999;Licht, 2003;Pahkala et al, 2000;Van Uitregt et al, 2007). Moreover, many of these habitats suffer pollution from nitrogen compounds, pesticides, herbicides, and metals (Ortiz-Santaliestra et al, 2006;Berger, 1989;Ortiz et al, 2004;Brühl et al, 2013;Linder and Grillitsch, 2000;Ortiz-Santaliestra et al, 2005;Gillardin et al, 2009;Adlassnig et al, 2013;Chen et al, 2009;James et al, 2004;Hayes et al, 2002;Chen et al, 2006Chen et al, , 2007C...…”