Chronic gastritis in tigers associated with Helicobacter acinonyx

Schröder, H. D.; Ludwig, Christian; Jakob, W.; Reischl, Udo; Stolte, Manfred; Lehn, Norbert

doi:10.1016/s0021-9975(98)80072-8

Cited by 18 publications

(19 citation statements)

References 5 publications

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“…Cleavage of the amplified products by genus specific primer (399 bp) using Hinf I and In none mucosal sample bacteria resembling H. pylori or H. acinonyx were observed as previously reported (6,19,24).…”

Section: Resultssupporting

confidence: 77%

“…infection in semi or captive feline (10,19). However, in our study small lymphoid follicles were seen in three animals, In conclusion, the present study shows that captive Helicobacter species in neotropical Felidae neotropical Brazilian feline are commonly infected by H.…”

Section: Resultscontrasting

confidence: 52%

“…Helicobacter acinonyx was implicated in gastritis in tigers (19). In a retrospective survey conducted with 69 captive cheetahs, 40% of the deaths were associated with severe gastritis induced by Helicobacter spp.…”

Section: In Several Studies Gastritis Was Associated Withmentioning

confidence: 99%

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Gastric Helicobacter spp. infection in captive neotropical Brazilian feline

Camargo¹,

Uenaka²,

Motta³

et al. 2011

Braz. J. Microbiol.

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Ten captive neotropical Brazilian feline were submitted to gastroscopic examination and samples of gastric mucosa from fundus, corpus and pyloric antrum were evaluated for the presence of Helicobacter species.Warthin-Starry (WS) staining and PCR assay with species-specific primers and enzymatic cleavage were applied for bacterial detection and identification. Histological lesions were evaluated by haematoxylin and eosin staining. All animals showed normal gross aspect of gastric mucosa. Helicobacter heilmannii was confirmed in 100% of the samples by WS and PCR assay. Mild lymphocytic infiltrate in the lamina propria was observed in eight animals, mainly in the fundus region. Small lymphoid follicles were seen in three animals. No significant association between Helicobacter infection and histological findings was verified.These observations suggest that gastric Helicobacter spp. could be a commensal or a eventual pathogen to captive neotropical feline, and that procedures, way life, and stress level on the shelter apparently had no negative repercussion over the integrity of the stomach.

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Section: Resultssupporting

confidence: 77%

Section: Resultscontrasting

confidence: 52%

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Gastric Helicobacter spp. infection in captive neotropical Brazilian feline

Camargo¹,

Uenaka²,

Motta³

et al. 2011

Braz. J. Microbiol.

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“…infecting the chee- tah population as a whole. H. acinonychis has been isolated from other species of exotic felids and may have been transmitted to the cheetahs in the previously studied collection (4,35). Alternatively, these bacteria may have historically been more prevalent in cheetahs but become less so due to selective breeding efforts in captive institutions and maternal transmission of other Helicobacter spp.…”

Section: Discussionmentioning

confidence: 99%

Comparison ofHelicobacterspp. in Cheetahs (Acinonyx jubatus) with and without Gastritis

Terio¹,

Munson²,

Marker

et al. 2005

J Clin Microbiol

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Chronic gastritis causes significant morbidity and mortality in captive cheetahs but is rare in wild cheetahs despite colonization by abundant spiral bacteria. This research aimed to identify the Helicobacter species that were associated with gastritis in captive cheetahs but are apparently commensal in wild cheetahs. Helicobacter species were characterized by PCR amplification and sequencing of the 16S rRNA, urease, and cagA genes and by transmission electron microscopy of frozen or formalin-fixed paraffin-embedded gastric samples from 33 cheetahs infected with Helicobacter organisms (10 wild without gastritis and 23 captive with gastritis). Samples were screened for mixed infections by denaturant gel gradient electrophoresis of the 16S rRNA gene and by transmission electron microscopy. There was no association between Helicobacter infection and the presence or severity of gastritis. Eight cheetahs had 16S rRNA sequences that were most similar (98 to 99%) to H. pylori. Twenty-five cheetahs had sequences that were most similar (97 to 99%) to "H. heilmannii" or H. felis. No cheetahs had mixed infections. The ultrastructural morphology of all bacteria was most consistent with "H. heilmannii," even when 16S rRNA sequences were H. pylori-like. The urease gene from H. pylori-like bacteria could not be amplified with primers for either "H. heilmannii" or H. pylori urease, suggesting that this bacteria is neither H. pylori nor "H. heilmannii." The cagA gene was not identified in any case. These findings question a direct role for Helicobacter infection in the pathogenesis of gastritis and support the premise that host factors account for the differences in disease between captive and wild cheetah populations.Since the initial isolation of Helicobacter pylori from humans and its association with gastritis and peptic ulceration (23), Helicobacter spp. have been isolated from an ever-expanding range of host species (38). While Helicobacter pylori infection in humans has been associated with chronic gastritis, peptic ulceration, gastric adenocarcinoma, and lymphoma (23,31,32), Helicobacter pathogenicity in many species is less clear.

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“…5,[9][10][11]38,39,54 This reaction is unlike that seen in free-ranging cheetahs or other felid species (both domestic and zoo held) that usually only develop mild inflammatory lesions when infected with similar Helicobacter. 20,24,25,27,29,30,40,44,49,54,58 The lesions in cheetahs occur predominantly in the gastric fundus and are characterized by florid lymphocyte and plasma cell infiltrates, infiltration of glandular epithelium, parietal cell apoptosis and, in more chronic cases, may include gland hyperplasia, goblet cell metaplasia, fibrosis and/or atrophy. 5,10,38,39 Because no single strain of Helicobacter has been associated with gastritis in captive cheetahs 54 and the magnitude of the immune response differs considerably between captive and free-ranging cheetahs infected with similar bacteria, the pathogenesis of cheetah gastritis is suspected to be attributable to the nature of the immune response rather than bacterial factors alone.…”

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confidence: 99%

Characterization of the Gastric Immune Response in Cheetahs (Acinonyx jubatus) WithHelicobacter-Associated Gastritis

2011

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Captive cheetahs have an unusually severe progressive gastritis that is not present in wild cheetahs infected with the same strains of Helicobacter. This gastritis, when severe, has florid lymphocyte and plasma cell infiltrates in the epithelium and lamina propria with gland destruction, parietal cell loss, and, in some cases, lymphoid follicles. The local gastric immune response was characterized by immunohistochemistry in 21 cheetahs with varying degrees of gastritis. The character of the response was similar among types of gastritis except that cheetahs with severe gastritis had increased numbers (up to 70%) of lamina proprial CD79aþCD21-B cells. CD3þCD4þ T cells were present in the lamina propria, and CD3þCD8aþ T cells were within the glandular epithelium. Lymphoid aggregates had follicular differentiation with a central core of CD79aþ/CD45Rþ B cells and with an outer zone of CD3þ T cells that expressed both CD4 and CD8 antigens. MHC II antigens were diffusely expressed throughout the glandular and superficial epithelium. No cheetah had evidence of autoantibodies against the gastric mucosa when gastric samples from 30 cheetahs with different degrees of gastritis were incubated with autologous and heterologous serum. These findings indicate that T-cell distribution in cheetahs is qualitatively similar to that in other species infected with Helicobacter but that large numbers of lamina propria activated B cells and plasma cells did distinguish cheetahs with severe gastritis. Further research is needed to determine whether alterations in the Th1:Th2 balance are the cause of this more plasmacytic response in some cheetahs.

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Chronic gastritis in tigers associated with Helicobacter acinonyx

Cited by 18 publications

References 5 publications

Gastric Helicobacter spp. infection in captive neotropical Brazilian feline

Gastric Helicobacter spp. infection in captive neotropical Brazilian feline

Comparison ofHelicobacterspp. in Cheetahs (Acinonyx jubatus) with and without Gastritis

Characterization of the Gastric Immune Response in Cheetahs (Acinonyx jubatus) WithHelicobacter-Associated Gastritis

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