Chronic Stimulation–Induced Changes in the Rodent Thyroarytenoid Muscle

McMullen, Colleen A.; Butterfield, Timothy A.; Dietrich, Maria; Andreatta, Richard D.; Andrade, Francisco H.; Fry, Lisa; Stemple, Joseph C.

doi:10.1044/1092-4388(2010/10-0127)

Cited by 22 publications

(13 citation statements)

References 40 publications

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“…Muscles require different isoenzymes at different ages. Similar results to the 14-day group finding have been shown in a previous publication where we electrically stimulated the recurrent laryngeal nerve (McMullen et al 2011). If there is a reduction in glycogen, then there will be an impairment of glycogen breakdown needed for muscle metabolism.…”

Section: Discussionsupporting

confidence: 91%

“…With direct injection of NTF4, fiber size decreased at 30‐mo of age, similar to what we have previously observed after laryngeal nerve stimulation (McMullen et al. ). The time and dosage administration results suggest the need for further investigation to help explain fiber size change differences observed between systemic and direct application of NTF4.…”

Section: Discussionsupporting

confidence: 89%

“…Previous data show that aging rat muscle fibers change in the opposite direction compared to the muscles that were treated systemically with NTF4 (McMullen and Andrade 2006). With direct injection of NTF4, fiber size decreased at 30-mo of age, similar to what we have previously observed after laryngeal nerve stimulation (McMullen et al 2011). The time and dosage administration results suggest the need for further investigation to help explain fiber size change differences observed between systemic and direct application of NTF4.…”

Section: Discussionsupporting

confidence: 85%

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Enhancement of aging rat laryngeal muscles with endogenous growth factor treatment

et al. 2016

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Clinical evidence suggests that laryngeal muscle dysfunction is associated with human aging. Studies in animal models have reported morphological changes consistent with denervation in laryngeal muscles with age. Life‐long laryngeal muscle activity relies on cytoskeletal integrity and nerve–muscle communication at the neuromuscular junction (NMJ). It is thought that neurotrophins enhance neuromuscular transmission by increasing neurotransmitter release. We hypothesized that treatment with neurotrophin 4 (NTF4) would modify the morphology and functional innervation of aging rat laryngeal muscles. Fifty‐six Fischer 344xBrown Norway rats (6‐ and 30‐mo age groups) were used to evaluate to determine if NTF4, given systemically (n = 32) or directly (n = 24), would improve the morphology and functional innervation of aging rat thyroarytenoid muscles. Results demonstrate the ability of rat laryngeal muscles to remodel in response to neurotrophin application. Changes were demonstrated in fiber size, glycolytic capacity, mitochondrial, tyrosine kinase receptors (Trk), NMJ content, and denervation in aging rat thyroarytenoid muscles. This study suggests that growth factors may have therapeutic potential to ameliorate aging‐related laryngeal muscle dysfunction.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 85%

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Enhancement of aging rat laryngeal muscles with endogenous growth factor treatment

et al. 2016

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“…However, choosing the right stimulation parameters, and the daily pattern of stimulation sessions requires considerable care and further research. McMullen et al achieved an increase of neuromuscular junction density and mitochondrial content via electrical stimulation in a small rodent model, but essential parameters, such as mean muscle fiber diameter decreased [31]. In this case the stimulation represented 1.67% of total time whereas we used stimulation for 0.14% of total time.…”

Section: Discussionmentioning

confidence: 96%

Reversing Age Related Changes of the Laryngeal Muscles by Chronic Electrostimulation of the Recurrent Laryngeal Nerve

et al. 2016

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Age related atrophy of the laryngeal muscles -mainly the thyroarytenoid muscle (TAM)- leads to a glottal gap and consequently to a hoarse and dysphonic voice that significantly affects quality of life. The aim of our study was to reverse this atrophy by inducing muscular hypertrophy by unilateral functional electrical stimulation (FES) of the recurrent laryngeal nerve (RLN) in a large animal model using aged sheep (n = 5). Suitable stimulation parameters were determined by fatiguing experiments of the thyroarytenoid muscle in an acute trial. For the chronic trial an electrode was placed around the right RLN and stimulation was delivered once daily for 29 days. We chose a very conservative stimulation pattern, total stimulation time was two minutes per day, or 0.14% of total time. Overall, the mean muscle fiber diameter of the stimulated right TAM was significantly larger than the non-stimulated left TAM (30μm±1.1μm vs. 28μm±1.1 μm, p<0.001). There was no significant shift in fiber type distribution as judged by immunohistochemistry. The changes of fiber diameter could not be observed in the posterior cricoarytenoid muscle (PCAM).FES is a possible new treatment option for reversing the effects of age related laryngeal muscle atrophy.

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“…From what is known about the histologic properties of the intrinsic laryngeal muscles, namely the TA‐LCA muscle complex, they are considered to be fatigue resistant, consisting more of fast‐contracting type II fibers (with more IIA than IIX) than slow‐contracting type I fibers 14, 15, 21. Although results of a recent study provide evidence that the thyroarytenoid muscle shows response changes and remodeling to a more glycolytic (fatigue‐resistant) capacity with constant stimulation, the most substantial limitation remains that there is no method to visualize how the muscle is responding during stimulation 21. Our study demonstrates that VLS is a tool that may allow the measurement of laryngeal muscle physiology in vivo in both health and disease.…”

Section: Discussionmentioning

confidence: 99%

In vivo oxygen consumption and hemoglobin levels in human thyroarytenoid muscle

et al. 2011

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Objectives/Hypothesis Visible light spectroscopy (VLS) is the technology behind the Food and Drug Administration–approved TSTAT device that is used to monitor tissue oxygen (StO2) and relative total hemoglobin (rtHb) levels by measuring reflected visible light. The purpose of this novel, pilot study was to determine if VLS is a reliable and valid method of measuring StO2 and rtHb levels in the human thyroarytenoid/lateral cricoarytenoid (TA-LCA) muscle complex, thus providing information about vocal fold muscle physiology. Study Design Pre-test/post-test with mulitple baselines and two conditions. Methods VLS measurements were taken at baseline, during exercise, and following recovery on six subjects using both noncontact channel-port endoscope (endo-probe) and laryngeal electromyography (LEMG) needle-guided techniques. Results The average baseline StO2 was 69% (standard deviation [SD] = 3.6%) for the LEMG-guided probe and was 71.5% (SD = 2.8%) for the endo-probe. During phonation, the StO2 for the LEMG-guided probe dropped to 59% (SD = 7%; P = .04). Mean rtHb measured by the LEMG probe rose from a baseline of 144 µM (SD = 165 µM) to 214 µM (SD = 166 µM, P = .34) during phonation and back to 149 µM (SD = 139 µM, P = .85) after recovery. Mean rtHb as measured using the endo-probe at baseline and after recovery was 104 µM (SD = 30 µM, P = .76). Conclusions VLS can be used to measure changes in StO2 and rtHb levels pre- and postexercise in the human TA-LCA muscle complex.

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Chronic Stimulation–Induced Changes in the Rodent Thyroarytenoid Muscle

Abstract: Rat thyroarytenoid muscles are capable of remodeling in response to chronic electrical stimulation.

Cited by 22 publications

References 40 publications

Enhancement of aging rat laryngeal muscles with endogenous growth factor treatment

Enhancement of aging rat laryngeal muscles with endogenous growth factor treatment

Reversing Age Related Changes of the Laryngeal Muscles by Chronic Electrostimulation of the Recurrent Laryngeal Nerve

In vivo oxygen consumption and hemoglobin levels in human thyroarytenoid muscle

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