Chryseobacterium Indologenes as a Novel Cause of       Bacteremia in a Neonate

Mirza, Irfan Ali; Khalid, Ayesha; Hameed, Fatima; Imtiaz, Anam; Ashfaq, Ambar; Tariq, Anam

doi:10.29271/jcpsp.2019.04.375

Cited by 7 publications

(6 citation statements)

References 6 publications

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“…Liver cirrhosis [4] Tracheostomy [41]; 48.2 Urinary tract infection [9]; Pneumonia [7]; Bacteremia [6]; Central nervous system infection [1] Organ cancer [5]; Leukemia [4]; AlloHSCT [2]; Chemotherapy [2]; Post-renal transplantation [1] CVC [1]; Ventilator [2]; 3.4 Pneumonia [15]; Bacteremia [1] Cystic fibrosis [15]; Operated on for craniopharyngioma [1] CVC [1]; Ventilator [1] Ciprofloxacin (100%), levofloxacin (100%), TMP/SMZ (100%), piperacillin-tazobactam (100%), ceftazidime (100%), cefepime (100%), imipenem (81.2%), meropenem (81.2%), amikacin (25%), gentamicin (18.8%) [6]; Ventilation-associated pneumonia [2]; Urinary tract infection [4] Diabetes mellitus [7]; Hypertension [9]; History of stroke [4]; Coronary artery disease [5]; End-stage renal disease [3] Tracheostomy [4]; Central line catheter [8]; Recent surgery [9]; Urinary catheter [12] TMP/SMZ (91.67%), levofloxacin (75%), ciprofloxacin (41.67%), piperacillin-tazobactam (16.67%).…”

Section: Discussionmentioning

confidence: 99%

Clinical and molecular characteristics of Chryseobacterium indologenes isolates at a teaching hospital in Shanghai, China

Zhang¹,

Li²,

Yang³

et al. 2021

Ann Transl Med

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Background: Chryseobacterium indologenes (C. indologenes) has recently emerged as a cause of life-threatening nosocomial infections in humans. This study aims to investigate the clinical characteristics, homology, and antimicrobial patterns of C. indologenes clinical isolates at a teaching hospital in Shanghai, China.Methods: A total of 135 consecutive non-replicate clinical C. indologenes isolates from January 2010 to December 2018 were collected at a tertiary care university hospital in Shanghai, China. Genetic relatedness of the isolates was performed by pulsed-field gel electrophoresis (PFGE). The antimicrobial susceptibility of these isolates was measured by the microdilution broth method. The prevalence of β-lactamase genes was investigated by polymerase chain reaction (PCR), while the quinolone resistance-determining regions (QRDRs) were sequenced.Results: All 135 C. indologenes isolates were collected from hospitalized patients with an average age of 55 years. Most of these clinical isolates were derived from ascites (59.3%) or urine (23.7%) specimens. Eighty (80/135) of the strains were classified as clone D by PFGE. In vitro drug susceptibility tests showed that minocycline and trimethoprim-sulfamethoxazole had sound antibacterial effects. However, more than 86% of the tested strains were resistant to cephalosporins (ceftazidime, cefotaxime), β-lactamase/β-lactamase inhibitors (cefoperazone-sulbactam), and carbapenems (meropenem, imipenem). Metallo-β-lactamase bla IND and type A broad-spectrum β-lactamase genes bla CIA were present in 135 and 103 isolates, respectively. The clinical strains in our hospital mainly carried bla IND-2 (89.6%, 121/135). Compared with previous studies, these strains had a high rate of resistance to quinolones. The resistance rates to levofloxacin, ciprofloxacin, norfloxacin, gatifloxacin, and nemonoxacin were as high as 83.7-94.8%. The mutations at Ser83Val, Ser83Tyr, and Asp87Gly in the QRDRs of GyrA were significantly related to the resistance of C. indologenes to levofloxacin. All but one quinolone-resistant strain contained at least one significant mutation.Conclusions: This study showed a clonal dissemination of C. indologenes isolates in infections at a tertiary care university hospital in Shanghai, China. Minocycline and trimethoprim-sulfamethoxazole had favorable in vitro antibacterial effects. However, the high resistance rate to β-lactams and quinolones was due to carrying β-lactamase (bla IND , bla CIA ), and mutations in the QRDRs of GyrA.

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Section: Discussionmentioning

confidence: 99%

Clinical and molecular characteristics of Chryseobacterium indologenes isolates at a teaching hospital in Shanghai, China

Zhang¹,

Li²,

Yang³

et al. 2021

Ann Transl Med

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“…Although there has been an increasing trend of C. indologenes bacteremia related to infants over the last few decades ( 5 , 75 , 76 ), most cases of infections caused by C. indologenes are still reported in adults ( 8 , 10 ). The precise reason for fewer reports of C. indologenes infections in children remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Whole genome sequencing of the multidrug-resistant Chryseobacterium indologenes isolated from a patient in Brazil

et al. 2022

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Chryseobacterium indologenes is a non-glucose-fermenting Gram-negative bacillus. This emerging multidrug resistant opportunistic nosocomial pathogen can cause severe infections in neonates and immunocompromised patients. This study aimed to present the first detailed draft genome sequence of a multidrug-resistant C. indologenes strain isolated from the cerebrospinal fluid of an infant hospitalized at the Neonatal Intensive Care Unit of Brazilian Tertiary Hospital. We first analyzed the susceptibility of C. indologenes strain to different antibiotics using the VITEK 2 system. The strain demonstrated an outstanding resistance to all the antibiotic classes tested, including β-lactams, aminoglycosides, glycylcycline, and polymyxin. Next, C. indologenes was whole-genome-sequenced, annotated using Prokka and Rapid Annotation using Subsystems Technology (RAST), and screened for orthologous groups (EggNOG), gene ontology (GO), resistance genes, virulence genes, and mobile genetic elements using different software tools. The draft genome contained one circular chromosome of 4,836,765 bp with 37.32% GC content. The genomic features of the chromosome present numerous genes related to cellular processes that are essential to bacteria. The MDR C. indologenes revealed the presence of genes that corresponded to the resistance phenotypes, including genes to β-lactamases (blaIND–13, blaCIA–3, blaTEM–116, blaOXA–209, blaVEB–15), quinolone (mcbG), tigecycline (tet(X6)), and genes encoding efflux pumps which confer resistance to aminoglycosides (RanA/RanB), and colistin (HlyD/TolC). Amino acid substitutions related to quinolone resistance were observed in GyrA (S83Y) and GyrB (L425I and K473R). A mutation that may play a role in the development of colistin resistance was detected in lpxA (G68D). Chryseobacterium indologenes isolate harbored 19 virulence factors, most of which were involved in infection pathways. We identified 13 Genomic Islands (GIs) and some elements associated with one integrative and conjugative element (ICEs). Other elements linked to mobile genetic elements (MGEs), such as insertion sequence (ISEIsp1), transposon (Tn5393), and integron (In31), were also present in the C. indologenes genome. Although plasmids were not detected, a ColRNAI replicon type and the most resistance genes detected in singletons were identified in unaligned scaffolds. We provided a wide range of information toward the understanding of the genomic diversity of C. indologenes, which can contribute to controlling the evolution and dissemination of this pathogen in healthcare settings.

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“…Se encuentra en suelo, agua y superficies húmedas. Puede ocasionar infecciones en el ámbito hospitalario, sobre todo a pacientes con estado inmune alterado o portadores de dispositivos médicos [4][5][6][7][8][9] .…”

Section: Discussionunclassified

“…Todos los autores coinciden en que un rápido tratamiento, con la combinación de TMP/SMZ y ciprofloxacina, incidirá favorablemente en el pronóstico del paciente [4][5][6][7][8][9] .…”

Section: Introductionunclassified

Tigeciclina intraventricular como tratamiento de ventriculitis por Chryseobacterium indologenes multirresistente. A propósito de un caso pediátrico

Samudio-Domínguez¹,

Ortiz-Cuquejo²,

Gómez³

et al. 2021

Infect

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La ventriculitis por Chryseobacterium indologenes puede ser un reto terapéutico importante, sobre todo cuando el germen adquiere resistencia intra tratamiento a los antimicrobianos habituales. La tigeciclina intraventricular podría ser una excelente opción en estos casos. Se presenta el caso de una escolar que recibió tratamiento intraventricular con tigeciclina, ante la ausencia de alternativas terapéuticas, con suceso exitoso.

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Chryseobacterium Indologenes as a Novel Cause of Bacteremia in a Neonate

Abstract: The genus Chryseobacterium, a rare human pathogen, was first described in 1994 by Vandamme and colleagues

Cited by 7 publications

References 6 publications

Clinical and molecular characteristics of Chryseobacterium indologenes isolates at a teaching hospital in Shanghai, China

Clinical and molecular characteristics of Chryseobacterium indologenes isolates at a teaching hospital in Shanghai, China

Whole genome sequencing of the multidrug-resistant Chryseobacterium indologenes isolated from a patient in Brazil

Tigeciclina intraventricular como tratamiento de ventriculitis por Chryseobacterium indologenes multirresistente. A propósito de un caso pediátrico

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