Chryseobacterium takakiae sp. nov., a member of the phylum Bacteroidetes isolated from Takakia lepidozioides

Abstract

A Gram-stain-negative, rod-shaped and non-endospore-forming bacterium, designated strain AG1-2 T , was isolated from Takakia lepidozioides collected from the Gawalong glacier in Tibet,China and characterized using a polyphasic taxonomic approach. The predominant fatty acids of strain AG1-2 T were iso-C 15 : 0 (36.0 %), iso-C 17 : 0 3-OH (20.2 %), summed feature 9 (iso- orange. MK-6 is the major or only respiratory quinone and branched-chain fatty acids iso-C 15 : 0 , iso-C 17 : 0 3-OH and iso-C 17 : 1 v9c are the major fatty acids, and the genomic DNA G+C content is 29-39 mol% (Vandamme et al., 1994; Kämpfer et al., 2009). In this paper, we describe strain AG1-2 T , isolated from a kind of bryophyte, Takakia lepidozioides. The organism was related to the genus Chryseobacterium on the basis of 16S rRNA gene sequence comparison. The phenotypic, genetic and chemotaxonomic analyses showed that it represents a novel species of the genus Chryseobacterium.Samples of the bryophyte Takakia lepidozioides were collected from three representative sites in the Gawalong glacier zone in Tibet, China. About 1 g of surfacedisinfected plants (Li et al., 2011) was homogenized in 0.85 % (w/v) NaCl and serial standard dilutions were spread on trypticase soy agar (TSA; Difco). The plates were incubated at 28 u C for 2-3 days and one yellow-pigmented strain, designated AG1-2 T , was isolated and purified by transfer onto new plates. Strain AG1-2 T was maintained on TSA at 4 uC and preserved at 280 uC in trypticase soy broth (TSB; Difco) containing 30 % (v/v) glycerol. Biomass for chemotaxonomic and molecular systematic studies was prepared by growing the strain in TSB at 28 u C for 2 days. Cells were harvested by centrifugation, and freeze-dried pending analysis.

Chryseobacterium profundimaris sp. nov., a new member of the family Flavobacteriaceae isolated from deep-sea sediment

“…Strain DY46 T was found to be able to grow in TSB containing 3 % (w/v) NaCl, whereas C. takakiae AG1-2 T does not. Besides, the growth temperature range of C. takakiae AG1-2 T is 25-42°C (Zhao et al 2015), while strain DY46 T can grow at 4°C. Phenotypic characteristics that serve to differentiate strain DY46 T from other related Chryseobacterium species are listed in Tables 1, 2.…”

“…At the time of writing, the genus Chryseobacterium contained 84 species with validly published names. This list does not include Chryseobacterium takakiae, the description of which was published online when this paper was under review (Zhao et al 2015).…”

Chryseobacterium lecithinasegens sp. nov., a siderophore-producing bacterium isolated from soil at the bottom of a pond

“…In addition to soil, bacteria belonging to the genus Chryseobacterium are found in various environments, including fresh water and seawater as well as animal and human tissues. This adaptability is expected to be related to their high variation in siderophore systems [10][11][12][13].…”

Chryseobacterium lacus sp. nov. Isolated From the Surface Water of Two Lakes With Light-Induced Carotenoid Production

“…Currently, the genus Chryseobacterium comprises 110 species with validly published names 1 , among which Chryseobacterium gleum is the type species (Vandamme et al, 1994). The members of the genus Chryseobacterium have been isolated from a wide variety of habitats including plants, soil, roots of trees, clinical specimens, animal bodies, freshwater, wastewater, compost, sludge, sediments, and dairy products (Tai et al, 2006;Cho et al, 2010;Montero-Calasanz et al, 2013;Xu et al, 2015;Yang et al, 2015;Zhao et al, 2015).…”