Circadian and light induced expression of Per1 and Per2 in the rat suprachiasmatic nucleus

Yan, Lily; Takekida, Shigeki; Takumi, Toru; Okamura, Hitoshi

doi:10.1016/s0168-0102(98)82295-5

Cited by 57 publications

(83 citation statements)

References 11 publications

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“…Instead, each cellular SCN oscillator has a characteristic phase, and the individual oscillators are temporally and spatially organized within the nucleus. During a circadian cycle in vivo, the clock genes Per1 and Per2 are first expressed in cells lying in the dorsomedial, periventricular part of the SCN (near the third ventricle) and then spread out to the central region and finally to the ventral region (Yan and Okamura 2002;Quintero et al 2003;Yamaguchi et al 2003). Although the majority of the SCN cellular oscillators (~50-60%) have similar phases, others do not (Quintero et al 2003;Yamaguchi et al 2003).…”

Section: Spatiotemporal Oscillations In the Scnmentioning

confidence: 99%

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Exploring Spatiotemporal Organization of SCN Circuits

Yan

Karatsoreos

LeSauter

et al. 2007

Cold Spring Harbor Symposia on Quantitative Biology

105

111

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Suprachiasmatic nucleus (SCN) neuroanatomy has been a subject of intense interest since the discovery of the SCN's function as a brain clock and subsequent studies revealing substantial heterogeneity of its component neurons. Understanding the network organization of the SCN has become increasingly relevant in the context of studies showing that its functional circuitry, evident in the spatial and temporal expression of clock genes, can be reorganized by inputs from the internal and external environment. Although multiple mechanisms have been proposed for coupling among SCN neurons, relatively little is known of the precise pattern of SCN circuitry. To explore SCN networks, we examine responses of the SCN to various photic conditions, using in vivo and in vitro studies with associated mathematical modeling to study spatiotemporal changes in SCN activity. We find an orderly and reproducible spatiotemporal pattern of oscillatory gene expression in the SCN, which requires the presence of the ventrolateral core region. Without the SCN core region, behavioral rhythmicity is abolished in vivo, whereas low-amplitude rhythmicity can be detected in SCN slices in vitro, but with loss of normal topographic organization. These studies reveal SCN circuit properties required to signal daily time.

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Section: Spatiotemporal Oscillations In the Scnmentioning

confidence: 99%

“…Depending on which data one examines, it appears that the spatial change can be described as either a wave (Figs. 6 and 7) (Yan and Okamura 2002) or a tide (Figs. 7 and 8) (Hamada et al 2004).…”

Section: Space and Time In Scn Circuits: Tides And Wavesmentioning

confidence: 99%

Exploring Spatiotemporal Organization of SCN Circuits

Yan

Karatsoreos

LeSauter

et al. 2007

Cold Spring Harbor Symposia on Quantitative Biology

105

111

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“…Ces données électrophysiolo-giques sont appuyées par des études sur la distribution des ARNm des gènes de l'horloge au cours d'un cycle circadien. En effet, lorsqu'ils sont à leur maximum d'expression, durant le jour, les ARNm des gènes Per1-2 sont principalement observés dans la partie dorsomédiane (neurones à AVP) des NSC chez le hamster doré [17], le rat [18,19] et la souris [20]; la proportion de neurones à VIP exprimant ces gènes est quant à elle beaucoup plus faible dans toutes ces espèces. Toutefois, les neurones des parties ventrolatérale et dorsomédiane présentent une expression rythmique suivant la même phase, du moins chez le rat [19].…”

Section: Brève Histologie Des Nscunclassified

“…En effet, lorsqu'ils sont à leur maximum d'expression, durant le jour, les ARNm des gènes Per1-2 sont principalement observés dans la partie dorsomédiane (neurones à AVP) des NSC chez le hamster doré [17], le rat [18,19] et la souris [20]; la proportion de neurones à VIP exprimant ces gènes est quant à elle beaucoup plus faible dans toutes ces espèces. Toutefois, les neurones des parties ventrolatérale et dorsomédiane présentent une expression rythmique suivant la même phase, du moins chez le rat [19]. L'utilisation de techniques telles que l'hybridation in situ non radioactive [19,21] Figure 1, l'horloge des NSC continue de fonctionner en l'absence de repère temporel (obscurité constante).…”

Section: Brève Histologie Des Nscunclassified

“…Toutefois, les neurones des parties ventrolatérale et dorsomédiane présentent une expression rythmique suivant la même phase, du moins chez le rat [19]. L'utilisation de techniques telles que l'hybridation in situ non radioactive [19,21] Figure 1, l'horloge des NSC continue de fonctionner en l'absence de repère temporel (obscurité constante). Sa période est alors différente (bien que proche) de 24 heures.…”

Section: Brève Histologie Des Nscunclassified

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Les noyaux suprachiasmatiques : une horloge circadienne composée

Dardente

Cermakian

2005

Med Sci (Paris)

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Expression of VIP and/or PACAP receptor mRNA in peptide synthesizing cells within the suprachiasmatic nucleus of the rat and in its efferent target sites

Κalamatianos

Kalló

Piggins

et al. 2004

J of Comparative Neurology

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The suprachiasmatic nucleus (SCN) contains the predominant circadian pacemaker in mammals. Considerable evidence indicates that VPAC(2) and PAC(1), receptors for vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP), play critical roles in maintaining and entraining circadian rhythms. Retinal projections to the rat SCN contain PACAP and terminate mostly in the ventral SCN, the site of VIP neurons. The incidence of VPAC(2) and PAC(1) mRNAs within distinct neuronal populations of the rat SCN has been determined using double-label in situ hybridization. VPAC(2) mRNA was detected in almost all arginine-vasopressin (AVP) neurons of the dorsomedial SCN and in 41% of the VIP neurons; somatostatin (SST) neurons, predominantly in dorsomedial and intermediate regions, showed a decreased incidence (23%). PAC(1) mRNA was present in nearly half of the VIP and SST neurons (45% and 40%, respectively) and in one-third of the AVP neurons (32%). Cells expressing VPAC(2) mRNA also were detected in diencephalic areas that receive VIP-immunoreactive SCN efferents, such as the peri-suprachiasmatic region, lateral subparaventricular zone, parvocellular hypothalamic paraventricular subdivisions, dorsomedial hypothalamic nucleus, and anterior thalamic paraventricular and paratenial nuclei. The extensive distribution of PAC(1) mRNA within the SCN suggests that actions of PACAP are not restricted to the predominantly retinorecipient region. The presence of VPAC(2) mRNA in nearly half the VIP neurons, in almost all the AVP neurons, and at sites receiving VIP-immunoreactive SCN efferents suggests that the SCN VIP neurons are coupled and/or autoregulated and also influence the AVP-containing dorsomedial SCN and distal sites via VPAC(2).

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Circadian and light induced expression of Per1 and Per2 in the rat suprachiasmatic nucleus

Cited by 57 publications

References 11 publications

Exploring Spatiotemporal Organization of SCN Circuits

Exploring Spatiotemporal Organization of SCN Circuits

Les noyaux suprachiasmatiques : une horloge circadienne composée

Expression of VIP and/or PACAP receptor mRNA in peptide synthesizing cells within the suprachiasmatic nucleus of the rat and in its efferent target sites

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